Home>>Proteins>> CD Antigens>> CD14>>CD14 Mouse

CD14 Mouse

目录号 : GP22995

CD14 Mouse Recombinant

CD14 Mouse Chemical Structure

规格 价格 库存 购买数量
2μg
¥2,450.00
5-10工作日
5μg
¥3,780.00
5-10工作日
10μg
¥7,280.00
5-10工作日

电话:400-920-5774 Email: sales@glpbio.cn

Customer Reviews

Based on customer reviews.

Sample solution is provided at 25 µL, 10mM.

产品文档

Quality Control & SDS

View current batch:

产品描述

The CD14 is produced from mouse CD14 transfected CHO-cells in serum free medium. Before transfection the complete mouse CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR). The recombinant mouse CD-14 was purified by His-tag and controlled by SDS page.The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 is the 50 kDa protein.

Product Data

Purity Source CHO-cells.
Phycical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Shipping Condition Shipped at Room temp.
Synonyms Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD14.
Solubility It is recommended to reconstitute the lyophilized Mouse CD14 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Mouse CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution Mouse CD14 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation CD14 was lyophilized from a concentrated protein solution (1 mg/ml) containing phosphate-buffered saline, pH 7.2.

Introduction

CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 has been shown to bind apoptotic cells.

Applications

? ELISA. ? Inhibition Assays. ? Western Blotting.

Stability

Lyophilized Mouse CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution Mouse CD14 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.