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β-Amyloid (1-42), rat TFA Sale

目录号 : GC34242

β-Amyloid(1-42),ratTFA是由42个氨基酸组成的多肽,对海马切片有毒性,可用于阿尔兹海默症的研究。

β-Amyloid (1-42), rat TFA Chemical Structure

规格 价格 库存 购买数量
500μg
¥1,785.00
现货
1mg
¥2,856.00
现货
5mg
¥10,710.00
现货

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Sample solution is provided at 25 µL, 10mM.

产品文档

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实验参考方法

Cell experiment:

After treating the hippocampal slices with 20 μM β-Amyloid (1-42) for 4 h, supernatant is replaced by fresh H-ASCF/3 (0.9 mL/chamber) to which 0.1 mL MTT stock solution (5 mg/mL H-ACSF/3) is added (MTT final concentration: 0.5 mg/mL). The chamber is left to rest for 15 min without carboxygenation. To stop further reduction of MTT, the medium (H-ACSF/3) is removed. The slices are transferred into 96-well plate, then pure DMSO (100 μL/slice/well) is added for dissolving formazane from the slices. (30 min in a 96-well plate). Then 70 μL DMSO solution from each slice (well) is transferred into another 96-well plate. The optical density (OD) of the dissolved formazane is measured at 550 and 620 nm[1].

References:

[1]. Mozes E, et al. A novel method for the rapid determination of beta-amyloid toxicity on acute hippocampal slices using MTT and LDH assays. Brain Res Bull. 2012 Apr 10;87(6):521-5.
[2]. Lagunes T, et al. Abeta(1-42) induces abnormal alternative splicing of tau exons 2/3 in NGF-induced PC12 cells. An Acad Bras Cienc. 2014 Dec;86(4):1927-34.

产品描述

β-Amyloid (1-42), rat TFA is a 42-aa peptide, shows cytotoxic effect on acute hippocampal slices, and used in the research of Alzheimer's disease.

β-Amyloid (1-42), rat shows cytotoxic effect on the hippocampal slices at 20 μM[1]. β-Amyloid (1-42), rat causes morphological changes in NGF-induced PC12 cells, induces formed cell processes to retract in differentiated cells and affect the expression of exons 2/3 in both undifferentiated and differentiated cells[2].

[1]. Mozes E, et al. A novel method for the rapid determination of beta-amyloid toxicity on acute hippocampal slices using MTT and LDH assays. Brain Res Bull. 2012 Apr 10;87(6):521-5. [2]. Lagunes T, et al. Abeta(1-42) induces abnormal alternative splicing of tau exons 2/3 in NGF-induced PC12 cells. An Acad Bras Cienc. 2014 Dec;86(4):1927-34.

Chemical Properties

Cas No. SDF
Canonical SMILES Asp-Ala-Glu-Phe-Gly-His-Asp-Ser-Gly-Phe-Glu-Val-Arg-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile-Ala
分子式 C199H307N53O59S.C2HF3O2 分子量 4532.04
溶解度 Water : < 0.1 mg/mL (insoluble) 储存条件 Store at -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

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1 mg 5 mg 10 mg
1 mM 0.2207 mL 1.1033 mL 2.2065 mL
5 mM 0.0441 mL 0.2207 mL 0.4413 mL
10 mM 0.0221 mL 0.1103 mL 0.2207 mL
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