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Avidin Protein

目录号 : GP22811

Avidin

Avidin Protein Chemical Structure

规格 价格 库存 购买数量
5mg
¥840.00
5-10工作日
25mg
¥2,030.00
5-10工作日
100mg
¥5,600.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

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产品描述

Avidin is a glycosylated polypeptide chain having a molecular mass of 68kDa and containing 4 subunits each with a binding site for biotin. The Avidin is purified by affinity chromatographic techniques.The purification procedure ensures minimal contamination by other proteins or DNA.The resulting high activity and purity of the product gives very low non-specific binding (NSB).

Product Data

Purity Source Hen's egg white.
Phycical Appearance Sterile Filtered white lyophilized powder. Shipping Condition Shipped at Room temp.
Synonyms Avidin; AVD; AVID.
Solubility It is recommended to reconstitute the lyophilized Avidin in sterile 18MΩ-cm H2O not less than 100µg/ml or more than 10mg/ml solutions.
Stability Lyophilized Avidin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution Avidin should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Biological Activity 14.6units/mg protein, 1 unit binds 1µg biotin.

Introduction

Avidin is a tetrameric protein of 4 identical subunits (homotetramer) each of which can bind to biotin with a high degree of affinity and specificity. Avidin molecular weight in its tetrameric form is estimated to be between 66-69 kDa. Avidin is produced in the oviducts of birds, reptiles and amphibians and is subsequently deposited in the whites of their eggs. In the chicken egg white, avidin makes up roughly 0.05% of total protein (approximately 1.8 mg per egg). 10% of Avidinбпs molecular weight is ascribed to carbohydrate content which is composed of four to five mannose and three N-acetylglucosamine residues. Avidin has at least three distinctive oligosaccharide structural type which are similar in structure and composition. The dissociation constant (KD) of avidin is approximately 10-15M, making it one of the strongest known non-covalent bonds.

Biological Activity

14.6units/mg protein, 1 unit binds 1µg biotin.

Applications

Avidin may be used to visualize biotin conjugated molecules in ELISA, blotting and histological techniques.

Stability

Lyophilized Avidin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution Avidin should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.