Ampicillin-d5
(Synonyms: 氨苄西林 d5) 目录号 : GC46849
An internal standard for the quantification of ampicillin
Cas No.:1426173-65-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Ampicillin-d5 is intended for use as an internal standard for the quantification of ampicillin by GC- or LC-MS. Ampicillin is a broad-spectrum antibiotic with activity against Gram-positive and Gram-negative bacteria, including veterinary isolates of S. pseudintermedius, S. aureus, E. coli, Pasteurella, and S. canis (MIC50s = 0.25, 0.5, 2, 0.12, and 0.25 μg/ml, respectively).1 In vivo, ampicillin (80 mg/kg, i.v.) reduces cough frequency, tachypnea, dyspnea, and fever and increases survival in a baboon (P. cynocephalus) model of pneumococcal pneumonia.2 Formulations containing ampicillin have been used to treat a variety of bacterial infections.
1.Awji, E.G., Damte, D., Lee, S.-J., et al.The in vitro activity of 15 antimicrobial agents against bacterial isolates from dogsJ. Vet. Med. Sci.74(8)1091-1094(2012) 2.Reyes, L.F., Restrepo, M.I., Hinojosa, C.A., et al.A non-human primate model of severe pneumococcal pneumoniaPLoS One11(11)e0166092(2016)
| Cas No. | 1426173-65-0 | SDF | |
| 别名 | 氨苄西林 d5 | ||
| Canonical SMILES | O=C(N[C@@H]1C(N2[C@]1([H])SC(C)(C)[C@@H]2C(O)=O)=O)C(N)C3=C([2H])C([2H])=C([2H])C([2H])=C3[2H] | ||
| 分子式 | C16H14D5N3O4S | 分子量 | 354.4 |
| 溶解度 | Water: Slightly Soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.8217 mL | 14.1084 mL | 28.2167 mL |
| 5 mM | 0.5643 mL | 2.8217 mL | 5.6433 mL |
| 10 mM | 0.2822 mL | 1.4108 mL | 2.8217 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
High-throughput analysis of tetracycline and penicillin antibiotics in animal tissues using electrospray tandem mass spectrometry with selected reaction monitoring transition
J Chromatogr A 2005 Dec 30;1100(2):193-9.PMID:16214156DOI:10.1016/j.chroma.2005.09.056
A simple, rapid, and simultaneous analysis method for oxytertracycline, tetracycline, chlortetracycline, penicillin G, ampicillin, and nafcillin in meat has been developed by using electrospray ionization tandem mass spectrometry. The sample preparation was performed by homogenizing with water followed by a centrifugal ultrafiltration, after addition of internal standards (demeclocycline, penicillin G-d5, Ampicillin-d5 and nafcillin-d6). The MS/MS analysis involves the combined use of sample enrichment on the short column and a multiple reaction monitoring technique. The overall recoveries from animal (bovine and swine) muscle, kidney, and liver fortified at the levels of 0.05 and 0.1 ppm ranged from 70 to 115% with the coefficients of variation ranging from 0.7 to 14.8% (n = 5). Analysis time, including sample preparation and determination, is only 3h per eight sample and detection limits for all antibiotics are 0.002 ppm. The method is considered to be satisfactory for the rapid screening of the tetracycline and penicillin antibiotic residues in meat.