Aminooxyacetic acid hemihydrochloride (Carboxymethoxylamine Hemihydrochloride)
(Synonyms: 氨氧基乙酸半盐酸盐; Carboxymethoxylamine hemihydrochloride; Aminooxyacetate hemihydrochloride) 目录号 : GC34064
A GABA-T inhibitor
Cas No.:2921-14-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Kinase experiment: |
Enzyme activity of aspartate transaminase is measured by a colorimetric assay assessing formation of pyruvate from oxaloacetate, a product of GOT1/2 (also called AST1/2) activity, as described previously. In brief, cells grown in 6-well plates are collected after 6, 24, or 48 hours of Aminooxyacetic acid hemihydrochloride (AOA) treatment and washed with cold PBS, lysed, and supernatant used for analysis[3]. |
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Cell experiment: |
Breast cancer cell lines are used in this study. Cells are plated in 96-well plates at 1,500 to 5,000 cells per well in 100 μL media. New medium with varying concentration of Aminooxyacetic acid hemihydrochloride (AOA) is added after 12 hours. The assay is performed after 48 hours[3]. |
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Animal experiment: |
Female albino rats (150 to 200 g) bred at our department are used. Aminooxyacetic acid hemihydrochloride (AOAA) is injected into a tail vein (2 mL/kg body weight). For this purpose the rat is placed in a plastic tube and the tail warmed in water 42°C[2]. |
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References: [1]. Wang C, et al. Malate-aspartate shuttle inhibitor aminooxyacetic acid leads to decreased intracellular ATP levels and altered cell cycle of C6 glioma cells by inhibiting glycolysis. Cancer Lett. 2016 Aug 1;378(1):1-7. |
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Aminooxyacetic acid is a GABA transaminase (GABA-T) inhibitor (Ki = 9.16 μM) that induces GABA accumulation in the brain.1 It also inhibits cystathionine β synthase (CBS) and cystathionine γ lyase (CSE; IC50s = 8.5 and 1.1 μM, respectively).2 Aminooxyacetic acid (100 mg/kg) decreases GABA-T activity, without affecting glutamic acid decarboxylase (GAD) activity, and increases GABA levels in all regions of rat brain.3 It also decreases GABA-T activity and increases GABA in mouse brain when administered at a dose of 13 mg/kg.4 Aminooxyacetic acid inhibits 3-mercaptopropionic acid-, strychnine-, and pentetrazole-induced seizures in mice (ED50s = 53, 130, and 85 mg/kg, respectively), however, it exhibits neurotoxicity in the chimney test (ED50 = 62 mg/kg) and is lethal (LD50 = 105 mg/kg) to mice.
1.Wallach, D.P.Studies on the GABA pathway. I. The inhibition of γ-aminobutyric acid-α-ketoglutaric acid transaminase in vitro and in vivo by U-7524 (amino-oxyacetic acid)Biochem. Pharmacol.5(4)323-331(1961) 2.Asimakopoulou, A., Panopoulos, P., Chasapis, C.T., et al.Selectivity of commonly used pharmacological inhibitors for cystathionine β synthase (CBS) and cystathionine γ lyase (CSE)Br. J. Pharmacol.169(4)922-932(2013) 3.L?scher, W., H?nack, D., and Gramer, M.Use of inhibitors of gamma-aminobutyric acid (GABA) transaminase for the estimation of GABA turnover in various brain regions of rats: A reevaluation of aminooxyacetic acidJ. Neurochem.53(6)1737-1750(1989) 4.L?scher, W.A comparative study of the pharmacology of inhibitors of GABA-metabolismNaunyn Schmiedebergs Arch. Pharmacol.315(2)119-128(1980)
| Cas No. | 2921-14-4 | SDF | |
| 别名 | 氨氧基乙酸半盐酸盐; Carboxymethoxylamine hemihydrochloride; Aminooxyacetate hemihydrochloride | ||
| Canonical SMILES | OC(CON)=O.[H]Cl.OC(CON)=O | ||
| 分子式 | NH2OCH2COOH · 0.5HCl | 分子量 | 109.3 |
| 溶解度 | DMSO : 42.9 mg/mL (392.50 mM) | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 9.1491 mL | 45.7457 mL | 91.4913 mL |
| 5 mM | 1.8298 mL | 9.1491 mL | 18.2983 mL |
| 10 mM | 0.9149 mL | 4.5746 mL | 9.1491 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Effects of barbiturates on the decerebrate rigidity in rats
J Pharmacobiodyn 1982 Sep;5(9):643-52.PMID:7153837DOI:10.1248/bpb1978.5.643
Effects of barbiturates (phenobarbital, mephobarbital, pentobarbital, hexobarbital and amobarbital) on the tonic and phasic responses of forelimbs were studied in the rats in which rigidity was induced by means of anemic decerebration. The inhibitory pattern of the tonic and phasic responses to phenobarbital was different from those to the other barbiturates, i.e. phenobarbital gradually inhibited both tonic and phasic responses, whereas other barbiturates suppressed significantly only the tonic response immediately after the administration of a relatively low dose (10 mg/kg) but hardly inhibited the phasic response unless at higher doses (20 and 40 mg/kg). Possible involvement of GABA-system in the inhibitory action of phenobarbital and pentobarbital on the rigidity was investigated. Since inhibition of the phasic response to pentobarbital was potentiated by the pretreatment of animals with Carboxymethoxylamine Hemihydrochloride (AOAA) and suppressed by the pretreatment with semicarbazide, it was suggested that GABAergic mechanism may be involved in the particular inhibition of phasic response to pentobarbital. However, the inhibitory action of phenobarbital appeared to be independent of GABA-system.