Home>>Signaling Pathways>> Others>> Others>>A-802715

A-802715 Sale

目录号 : GC33419

A802715是一种甲基黄嘌呤衍生物,TD50为0.9-1.1mM。

A-802715 Chemical Structure

Cas No.:107767-58-8

规格 价格 库存 购买数量
1mg
¥1,785.00
现货
5mg
¥3,570.00
现货
10mg
¥6,069.00
现货
20mg
¥10,710.00
现货

电话:400-920-5774 Email: sales@glpbio.cn

Customer Reviews

Based on customer reviews.

Sample solution is provided at 25 µL, 10mM.

产品文档

Quality Control & SDS

View current batch:

实验参考方法

Cell experiment:

DNA analysis is performed using a FACScan flow cytometer emitting a 488 nm beam. Red fluorescence from PI emission is collected as a linear signal through a 600 nm bandpass filter and recorded as a measure of total DNA content. Processing the red fluorescence into height, area and width (doublet discrimination mode) eliminated cell doublets. Data are collected in list mode and 10000 events are recorded per sample and displayed as a frequency distribution histogram. Estimates of the percentages of cells in the different periods of postirradiation incubation with marker statistics reveal the time at which the G2/M block is maximally expressed. These times are used for each cell line as a starting point at which the methylxanthine drugs were added. Cell debris, nuclei doublets and triplets were excluded by gating[1].

References:

[1]. Bohm L, et al. Influence of pentoxifylline, A-802710, propentofylline and A-802715 (Hoechst) on the expression of cell cycle blocks and S-phase content after irradiation damage. Biochim Biophys Acta. 2000 Dec 11;1499(1-2):1-10.

产品描述

A802715 is a methylxanthine derivative. A802715 has a TD50 (toxic dose of 50%) of 0.9-1.1 mM.

The toxicity of the methylxanthine derivative A802715 is determined against the two human melanoma lines, Be11 and MeWo, and against the two human squamous cell carcinoma lines, 4197 and 4451, by vital dye staining assay. A802715 has a TD50 of 0.9-1.1 mM and is the most toxic. In p53 wt cells BrdU incorporations show that the irradiation-induced suppression of S-phase entry is strongly enhanced by A802715. A802715 prolongs the G2/M block or remain ineffective depending on the p53 status of the cell line[1].

[1]. Bohm L, et al. Influence of pentoxifylline, A-802710, propentofylline and A-802715 (Hoechst) on the expression of cell cycle blocks and S-phase content after irradiation damage. Biochim Biophys Acta. 2000 Dec 11;1499(1-2):1-10.

Chemical Properties

Cas No. 107767-58-8 SDF
Canonical SMILES O=C(N1CCCCC(C)(O)C)N(C)C2=C(N(CCC)C=N2)C1=O
分子式 C16H26N4O3 分子量 322.4
溶解度 DMSO: 190 mg/mL (589.33 mM) 储存条件 Store at -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

制备储备液
1 mg 5 mg 10 mg
1 mM 3.1017 mL 15.5087 mL 31.0174 mL
5 mM 0.6203 mL 3.1017 mL 6.2035 mL
10 mM 0.3102 mL 1.5509 mL 3.1017 mL
  • 摩尔浓度计算器

  • 稀释计算器

  • 分子量计算器

质量
=
浓度
x
体积
x
分子量
 
 
 
*在配置溶液时,请务必参考产品标签上、MSDS / COA(可在Glpbio的产品页面获得)批次特异的分子量使用本工具。

计算

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % saline
计算重置