α-Carotene |
目录号 GC41183 |
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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Purity: >95.00%
- COA (Certificate Of Analysis)
- Datasheet
α-Carotene is a precursor of vitamin A that has been found in various fruits and vegetables.[1] It inhibits proliferation of GOTO human neuroblastoma cells more potently than β-carotene and halts the cell cycle at the G0/G1 phase concomitantly with a reduction in the mRNA expression of the protooncogene N-Myc.[2] It is also more potent than β-carotene in mouse models of skin and lung carcinogenesis and decreases the number of hepatomas in mice with spontaneous liver carcinogenesis when administered in drinking water at a concentration of 0.05%. [3] α-Carotene levels are increased in patients with coronary heart disease and are inversely correlated with the risk of estrogen receptor-negative breast cancer.[4][5]
Reference:
[1]. Bushway, R.J., and Wilson, A.M. Determination of α- and β-carotene in fruit and vegetables by high performance liquid chromatography. Can. Inst. Food Sci. Technol. J. 15(3), 165-169 (1982).
[2]. Murakoshi, M., Takayusa, J., Kimura, O., et al. Inhibitory effects of α-carotene on proliferation of the human neuroblastoma cell line GOTO. J. Natl. Cancer Inst. 81(21), 1649-1652 (1989).
[3]. Murakoshi, M., Nishino, H., Satomi, Y., et al. Potent preventive action of α-carotene against carcinogenesis: spontaneous liver carcinogenesis and promoting stage of lung and skin carcinogenesis in mice are suppressed more effectively by α-carotene than by β-carotene. Cancer Res. 52(23), 6583-6587 (1992).
[4]. Kontush, A., Spranger, T., Reich, A., et al. Lipophilic antioxidants in blood plasma as markers of atherosclerosis: The role of α-carotene and γ-tocopherol. Atherosclerosis 144(1), 117-122 (1999).
[5]. Zhang, X., Spiegelman, D., Baglietto, L., et al. Carotenoid intakes and risk of breast cancer defined by estrogen receptor and progesterone receptor status: A pooled analysis of 18 prospective cohort studies. Am. J. Clin. Nutr. 95(3), 713-725 (2012).
Cas No. | 7488-99-5 | SDF | |
别名 | all-trans-α-Carotene | ||
化学名 | (6'R)-β,ε-carotene | ||
Canonical SMILES | CC1(C)[C@@H](/C=C/C(C)=C/C=C/C(C)=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C2=C(C)CCCC2(C)C)C(C)=CCC1 | ||
分子式 | C40H56 | 分子量 | 536.9 |
溶解度 | DMF: 0.1 mg/ml, DMSO: 1 mg/ml, DMSO:PBS (pH 7.2) (1:10): 0.1 mg/ml | 储存条件 | Store at -20°C, protect from light, stored under nitrogen |
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. | ||
Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % ddH2O | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL ddH2O,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。