Home>>Signaling Pathways>> DNA Damage/DNA Repair>> Nucleoside Antimetabolite/Analogue>>5-BrdU

5-BrdU Sale

(Synonyms: 5-溴脱氧尿嘧啶核苷; BrdU; 5-Bromo-2'-deoxyuridine; BUdR) 目录号 : GC11940

A thymidine nucleoside derivative commonly used to identify proliferating cells

5-BrdU Chemical Structure

Cas No.:59-14-3

规格 价格 库存 购买数量
10mM (in 1mL DMSO)
¥431.00
现货
500mg
¥357.00
现货
1g
¥588.00
现货

电话:400-920-5774 Email: sales@glpbio.cn

Customer Reviews

Based on customer reviews.

Sample solution is provided at 25 µL, 10mM.

产品文档

Quality Control & SDS

View current batch:

实验参考方法

Vitro experiment [1]:

Samples

Nicotiana pith tissues

Preparation method

The solubility of this compound in DMSO is >15.4mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

5 μg/ml; 24 h

Applications

In nicotiana pith tissues, BrdU inhibited growth of pith explants, while thymidine completely reversed the inhibitory effect. The inhibition observed could be attributed to BrdU incorporation into DNA.

Animal experiment [2]:

Animal models

Pregnant NMRI mice

Dosage form

25 mg/kg (5 mg/ml dissolved in 0.1 M Tris-buffered saline, pH 7.6), i.p.; between embryonic days E11 and E17.

Application

In pregnant NMRI mice, a single pulse of 5-bromodeoxyuridine (BrdU) was injected between embryonic days E11 and E17 to label postmitotic neuroblasts. BrdU is an analog of Thy that is incorporated into the DNA of cells undergoing the S-phase and can be detected by polyclona or monoclonal antibodies. Immunoreactive nuclei were detected in the adult CNS after pulses of BrdU. BrdU could be used to map neuronal birthdates.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1] M. Durante, C. Geri, V. Nuti-Ronchi, G. Martini, E. Guillé, J. Grisvard, L. Giorgi, R. Parenti, M. Bulatti. Inhibition of Nicotiana glauca pich tissue proliferation through incorporation of 5-BrdU into SNA. Cell Differentiation, Volume 6, Issue 1, June 1977, Pages 53–63

[2] del Rio JA, Soriano E. Immunocytochemical detection of 5'-bromodeoxyuridine incorporation in the central nervous system of the mouse. Brain Res Dev Brain Res. 1989 Oct 1;49(2):311-7.

产品描述

5-Bromodeoxyuridine (5-BrdU) is an analog of Thy that is incorporated into the DNA of cells undergoing the S-phase and can be detected by monoclonal antibodies or polyclona111.

In vitro: The effect of 5-BrdU on the proliferation was studied in the early phases of dedifferentiation of Nicotiana glauca pith explants grown. It was found to be wholly inhibitory only when given during the first 72 h of culture, this inhibition being reversed by simultaneous addition of either deoxyeytidine or thymidine [1].

In vivo: Young adult offspring mice were stained for 5-BrdU using a monoclonal antibody and the peroxidase-antiperoxidase method. The distribution of immunoreactive nuclei was very similar to the previous [3H]thymidine data. The pattern of labelled nuclei at different embryonic ages followed the spatiotemporal gradients described for cortical and hippocampal neurogenesis. These data indicate that BrdU can be used to map neuronal birthdates. [2].

Clinical trials: Currenlty no clinical data are available.

References:
[1] M.  Durante, C. Geri, V. Nuti-Ronchi, G. Martini, E. Guillé, J. Grisvard, L. Giorgi, R. Parenti, M. Bulatti. Inhibition of Nicotiana glauca pich tissue proliferation through incorporation of 5-BrdU into SNA. Cell Differentiation, Volume 6, Issue 1, June 1977, Pages 53–63
[2] del Rio JA, Soriano E.   Immunocytochemical detection of 5'-bromodeoxyuridine incorporation in the central nervous system of the mouse. Brain Res Dev Brain Res. 1989 Oct 1;49(2):311-7.

5-Bromodeoxyuridine (5-BrdU) 是 Thy 的类似物,可掺入处于 S 期细胞的 DNA 中,可通过单克隆抗体或多克隆抗体检测。

体外:研究了 5-BrdU 对生长的青烟草髓外植体去分化早期阶段增殖的影响。它被发现仅在培养的前 72 小时内具有完全抑制作用,同时添加脱氧胞苷或胸苷可逆转这种抑制作用 [1]。

体内实验:使用单克隆抗体和过氧化物酶-抗过氧化物酶方法对年轻的成年后代小鼠进行 5-BrdU 染色。免疫反应核的分布与之前的 [3H] 胸苷数据非常相似。不同胚胎年龄的标记细胞核模式遵循皮质和海马神经发生所描述的时空梯度。这些数据表明 BrdU 可用于绘制神经元出生日期图。 [2].

临床试验:目前没有可用的临床数据。

Chemical Properties

Cas No. 59-14-3 SDF
别名 5-溴脱氧尿嘧啶核苷; BrdU; 5-Bromo-2'-deoxyuridine; BUdR
化学名 5-bromo-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione
Canonical SMILES BrC(C(N1)=O)=CN(C1=O)[C@@]2([H])C[C@@](O)([H])[C@@](O2)([H])CO
分子式 C9H11BrN2O5 分子量 307.1
溶解度 ≥ 15.35mg/mL in DMSO, ≥ 16.23 mg/mL in Water with ultrasonic, ≥ 11.56 mg/mL in 0.9% NS with ultrasonic 储存条件 4°C, protect from light
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

制备储备液
1 mg 5 mg 10 mg
1 mM 3.2563 mL 16.2813 mL 32.5627 mL
5 mM 0.6513 mL 3.2563 mL 6.5125 mL
10 mM 0.3256 mL 1.6281 mL 3.2563 mL
  • 摩尔浓度计算器

  • 稀释计算器

  • 分子量计算器

质量
=
浓度
x
体积
x
分子量
 
 
 
*在配置溶液时,请务必参考产品标签上、MSDS / COA(可在Glpbio的产品页面获得)批次特异的分子量使用本工具。

计算

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % saline
计算重置