Fetuin, Fetal Bovine Serum
(Synonyms: 胎球蛋白) 目录号 : GC60166
Fetuin, Fetal Bovine Serum is a glycoprotein from fetal bovine serum, which is often an important supplement to many serum-free media.
Cas No.:9014-81-7
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Animal experiment [1]: | |
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Animal models |
Male nude mice |
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Preparation Method |
The prostate adenocarcinoma cell PC-3 (2 million cells) were injected on the upper half of the dorsal thorax of the mice. The tumors were allowed to grow for 6 weeks. The mice in the control group (no treatment) received 0.1 ml saline intraperitoneally for 5 days and the mice in the treated group received 50 mg/kg Fetuin, Fetal Bovine Serum. Treatment started the day after tumor inoculation for 5 days. Six weeks after the inoculation of tumor, mice were killed and tumors were removed and weighed. |
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Dosage form |
Intraperitoneal injection, 50 mg/kg/d for 5d |
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Applications |
Tumors (average weight 325 mg) developed in 13 of 13 mice in the control group (treated with saline), none of the nine mice treated with fetuin (50 mg/kg) developed any tumor. |
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References: [1]: Yu C L, Tsai M H. Fetal fetuin selectively induces apoptosis in cancer cell lines and shows anti-cancer activity in tumor animal models[J]. Cancer letters, 2001, 166(2): 173-184. |
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Fetuin, Fetal Bovine Serum is a glycoprotein from fetal bovine serum, which is often an important supplement to many serum-free media. Bovine fetuin preparation has inhibited trypsin activity and promote cellular attachment, growth, and differentiation in many different culture systems [1]. Fetuins are established as a family within the cystatin superfamily [2].
Fetuin, Fetal Bovine Serum had activity on the S3 strain of HeLa cells for promoting cell attachment and stretching (flattening) on the glass [3]. Fetuin, Fetal Bovine Serum preparations stimulated the growth of a variety of mammalian cells from different species. Fetuin, Fetal Bovine Serum (30- 960 µg/ml) stimulated the growth of Hela cell [4]. The nuclei of the LNCaP cells that had been incubated with the Fetuin, Fetal Bovine Serum showed the characteristics of apoptosis [5].
Fetuin, Fetal Bovine Serum enhanced survival rate by up to 141% in the P388 leukemia model. In the PC-3 prostate cancer animal model, fetuin (50 mg/kg) seems to completely inhibit the formation of prostate cancer in mice [5].
References:
[1]. Nie Z. Fetuin: its enigmatic property of growth promotion[J]. American Journal of Physiology-Cell Physiology, 1992, 263(3): C551-C562.
[2]. Brown W M, Dziegielewska K M, Saunders N R, et al. Fetuin-an old friend revisited[J]. Bioessays, 1992, 14(11): 749-755.
[3]. Fisher H W, Puck T T, Sato G. Molecular growth requirements of single mammalian cells: The action of fetuin in promoting cell attachment to glass[J]. Proceedings of the National Academy of Sciences, 1958, 44(1): 4-10.
[4]. Marr A G M, Owen J A, Wilson G S. Studies on the growth-promoting glycoprotein fraction of foetal calf serum[J]. Biochimica et Biophysica Acta, 1962, 63(2): 276-285.
[5]. Yu C L, Tsai M H. Fetal fetuin selectively induces apoptosis in cancer cell lines and shows anti-cancer activity in tumor animal models[J]. Cancer letters, 2001, 166(2): 173-184.
| Cas No. | 9014-81-7 | SDF | |
| 别名 | 胎球蛋白 | ||
| 分子式 | 分子量 | ||
| 溶解度 | 储存条件 | Store at -20°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Calcium-binding properties of fetuin in fetal bovine serum
J Exp Zool1994 Dec 15;270(6):501-7.PMID: 7528258DOI: 10.1002/jez.1402700603
Fetuin, an abundant protein in fetal bovine plasma, is the bovine homolog of human alpha 2HS glycoprotein (alpha 2HS). In spite of numerous studies, the biological functions of both proteins remain elusive. We now report the remarkable 45Ca-binding activity of fetuin in fetal bovine serum that has been blotted on a membrane after electrophoresis. The Ca-binding ability of the purified protein was analyzed by equilibrium dialysis, which revealed that bovine fetuin had multiple Ca-binding sites, one of which had a Kd of 0.95 x 10(-4) M. It was also shown that the Ca-binding activity of fetuin was greater than that of albumin in serum of the bovine fetus at the late gestational stage. Since fetal bovine serum contains not only a high concentration of fetuin but also a high concentration of Ca, it is possible that fetuin functions to maintain high levels of Ca in fetal serum via normalization of the concentration of Ca2+ ions.
One-step affinity purification of fetuin from fetal bovine serum
Biotechnol Appl Biochem2002 Apr;35(2):83-9.PMID: 11916450DOI: 10.1042/ba20010067
Fetuin is a plasma glycoprotein widely distributed in mammals. It has been used as a model protein for structural analyses and investigations into the biological properties of glycoproteins. A convenient one-step procedure for biospecific isolation of fetuin from fetal bovine serum was developed on the basis of wheatgerm agglutinin (WGA) affinity separation. Two different porous supports, a silica-based material and a polymer-based material, were used for the immobilization of WGA. The prepared WGA adsorbents were characterized and process parameters of the affinity separation of fetuin were investigated and optimized. WGA was immobilized on silica and polymer supports with coupling yields of 99.6 and 99.4% respectively and amounts of coupled ligand of 7.9 and 9.2 mg of WGA/ml respectively. It has been shown that the specific capacities for fetuin were 5.1 mg/ml on WGA-silica, 1.8 mg/ml on WGA-polymer and 4.1 mg/ml on WGA-agarose. All three adsorbents proved to be suitable for the biospecific separation of fetuin. The polymer-based WGA adsorbent was successfully applied in the purification of fetuin from fetal bovine serum in a one-step separation process. The identity and purity of the isolated product was verified by SDS/PAGE. Under optimized conditions up to 21.6 mg of fetuin could be isolated from 1 ml of serum. The procedure described was designed to be easily scaled-up for the production of fetuin.