Home>>Signaling Pathways>> Proteases>> Endogenous Metabolite>>3-Methyladenine
3-Methyladenine 目录号 GC10710

Class III PI3K inhibitor

规格 价格 库存 购买数量
10mM (in 1mL DMSO)
¥389.00
现货
10mg
¥252.00
现货
50mg
¥357.00
现货

Customer Review

Based on customer reviews.

电话:400-920-5774 Email: sales@glpbio.cn

Sample solution is provided at 25 µL, 10mM.

质量管理

Quality Control & SDS

View current batch:

实验参考方法

Cell experiment [1]:

Cell lines

HT1080 cells

Preparation method

The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.

Reacting condition

5 or 10 mM; 10 hrs

Applications

3-Methyladenine (3-MA) (5 ~ 10 mM) strongly inhibited HT1080 cell migration. In addition, 10 mM 3-MA showed a stronger effect on the suppression of HT1080 cell migration than both 100 nM WMN and 25 μM LY294002.

References:

[1]. Ito S, Koshikawa N, Mochizuki S, et al. 3-Methyladenine suppresses cell migration and invasion of HT1080 fibrosarcoma cells through inhibiting phosphoinositide 3-kinases independently of autophagy inhibition. International journal of oncology, 2007, 31(2): 261-268.

Chemical Properties

Cas No. 5142-23-4 SDF
别名 3-MA
化学名 3-methylpurin-6-amine
Canonical SMILES CN1C=NC(=C2C1=NC=N2)N
分子式 C6H7N5 分子量 149.15
溶解度 ≥ 7.45mg/mL in DMSO, ≥ 5mg/mL in H2O 储存条件 Store at 2-8°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
  • 摩尔浓度计算器

  • 稀释计算器

质量
=
浓度
x
体积
x
分子量
 
 
 
*在配置溶液时,请务必参考产品标签上、MSDS / COA(可在Glpbio的产品页面获得)批次特异的分子量使用本工具。

计算

产品描述

3-Methyladenine is an inhibitor of class III phosphoinositide 3-kinase (PI3K) [1].

3-Methyladenine is widely used as an autophagy inhibitor through inhibiting class III PI3K without significantly affecting protein synthesis or ATP levels. 3-Methyladenine also inhibits class I PI3K. It is found that 3-Methyladenine has different effects on class I and class III PI3K. It blocks class I PI3K persistently while inhibits class III PI3K transiently. As an inhibitor of autophagy, 3-Methyladenine is usually used to study the role of autophagy. In addition, 3-Methyladenine is also found to have anti-cancer efficacy. It induces cell death of tumor under nutrient-starved conditions. In HT1080 cells, 3-Methyladenine significantly inhibits cell migration through reducing membrane ruffle and lamellipodia formation in normal culture condition. It also inhibits the invasion of HT1080 cells [1, 2].

References:
[1] Wu Y T, Tan H L, Shui G, et al. Dual role of 3-methyladenine in modulation of autophagy via different temporal patterns of inhibition on class I and III phosphoinositide 3-kinase. Journal of Biological Chemistry, 2010, 285(14): 10850-10861.
[2] Ito S, Koshikawa N, Mochizuki S, et al. 3-Methyladenine suppresses cell migration and invasion of HT1080 fibrosarcoma cells through inhibiting phosphoinositide 3-kinases independently of autophagy inhibition. International journal of oncology, 2007, 31(2): 261-268.