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Hydrocortisone 目录号 GC11551

A steroid hormone or glucocorticoid secreted by the adrenal cortex

规格 价格 库存 购买数量
10mM (in 1mL DMSO)
¥389.00
现货
50mg
¥515.00
现货

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Sample solution is provided at 25 µL, 10mM.

质量管理

Quality Control & SDS

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实验参考方法

Cell experiment [1]:

Cell lines

human lung microvascular endothelial cells

Preparation method

The solubility of this compound in DMSO is >13.3mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

4 or 6 μM; 16 h

Applications

In human lung microvascular endothelial cells, Hydrocortisone (HC) induced a concentration-dependent but biologically minor barrier enhancing and protective effect. The combination of HC (4 μM) and ascorbic acid (VitC) (1000 μM) pre- or post-treatment significantly reversed the LPS-induced barrier dysfunction.

Animal experiment [2]:

Animal models

6-hydroxydopamine (6-OHDA)-induced Parkinson’s disease (PD) mouse models

Dosage form

0.4 mg/kg; intraperitoneal injection, 7 consecutive days

Application

In Parkinson’s disease (PD) mouse models, Hydrocortisone caused approximate two-fold increase of parkin expression in selected brain subregions (striatum, ventral midbrain, and cerebellum), which was responsible for cell survival against oxidative stress.. Hydrocortisone also increased CREB expression. Hydrocortisone pretreatment markedly enhanced dopaminergic neuronal survival against 6-OHDA intoxication.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Barabutis N1, Khangoora V2, Marik PE2, et al. Hydrocortisone and Ascorbic Acid synergistically prevent and repair LPS- induced pulmonary endothelial barrier dysfunction. Chest. 2017 Jul 21. pii: S0012-3692(17)31276-X.

[2]. Ham S1, Lee YI2,3, Jo M1, et al. Hydrocortisone-induced parkin prevents dopaminergic cell death via CREB pathway in Parkinson's disease model. Sci Rep. 2017 Apr 3;7(1):525.

Chemical Properties

Cas No. 50-23-7 SDF
别名 N/A
化学名 (8S,9S,10R,11S,13S,14S,17R)-11,17-dihydroxy-17-(2-hydroxyacetyl)-10,13-dimethyl-2,6,7,8,9,11,12,14,15,16-decahydro-1H-cyclopenta[a]phenanthren-3-one
Canonical SMILES CC12CCC(=O)C=C1CCC3C2C(CC4(C3CCC4(C(=O)CO)O)C)O
分子式 C21H30O5 分子量 362.46
溶解度 ≥ 13.3mg/mL in DMSO 储存条件 Store at RT
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
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产品描述

Hydrocortisone is a steroid hormone or glucocorticoid secreted by the adrenal cortex.

Hydrocortisone (50 nM) shows a dose-dependent down-regulation of GR transcript in hCMEC/D3 cells. Hydrocortisone supplementation of the serum-reduced cell differentiation medium leads to a significant increase in TER across the hCMEC/D3 monolayer[1]. Hydrocortisone-treated Dendritic cells (DCs) show a decreased expression of MHC II molecules, the costimulatory molecule CD86, and the DC-specific marker CD83, as well as a strongly reduced IL-12 secretion. Hydrocortisone-treated DCs inhibit production of IFN-γ but induce an increased release of IL-4 and no change in IL-5[2]. Hydrocortisone reduces postischemic oxidative stress, perfusion pressure, and transudate formation. Hydrocortisone inhibits postischemic shedding of syndecan-1, heparan sulfate, and hyaluronan as is release of histamine from resident mast cells[3].

References:
[1]. F rster C, et al. Differential effects of hydrocortisone and TNFalpha on tight junction proteins in an in vitro model of the human blood-brain barrier. J Physiol. 2008 Apr 1;586(7):1937-49.
[2]. Bellinghausen I, et al. Inhibition of human allergic T-cell responses by IL-10-treated dendritic cells: differences from hydrocortisone-treated dendritic cells. J Allergy Clin Immunol. 2001 Aug;108(2):242-9.
[3]. Chappell D, et al. Hydrocortisone preserves the vascular barrier by protecting the endothelial glycocalyx. Anesthesiology. 2007 Nov;107(5):776-84.