α-Muricholic Acid-d4
(Synonyms: ω-MCA-d4) 目录号 : GC49008
An internal standard for the quantification of α-muricholic acid
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
α-Muricholic acid-d4 is intended for use as an internal standard for the quantification of α-muricholic acid by GC- or LC-MS. α-Muricholic acid is a murine-specific primary bile acid.1,2 Dietary administration of soybean protein decreases fecal levels of α-muricholic acid in mice with high-fat diet-induced obesity, which correlates with increases in fecal Clostridium cluster XIVa, a major producer of secondary bile acids.3 Plasma, liver, and muscle levels of α-muricholic acid are increased in mice switched from a high-fat to low-fat diet.4
1.Eyssen, H.J., Parmentier, G.G., and Mertens, J.A.Sulfate bile acids in germ-free and conventional miceEur. J. Biochem.66(3)507-514(1976) 2.Uehara, T., Xi Peng, X., Bennett, B., et al.c-Jun N-terminal kinase mediates hepatic injury after rat liver transplantationTransplantation78(3)324-332(2004) 3.Watanabe, K., Igarashi, M., Li, X., et al.Dietary soybean protein ameliorates high-fat diet-induced obesity by modifying the gut microbiota-dependent biotransformation of bile acidsPLoS One13(8)e0202083(2018) 4.La Frano, M.R., Hernandez-Carretero, A., Weber, N., et al.Diet-induced obesity and weight loss alter bile acid concentrations and bile acid-sensitive gene expression in insulin target tissues of C57BL/6J miceNutr. Res.4611-21(2017)
| Cas No. | N/A | SDF | |
| 别名 | ω-MCA-d4 | ||
| Canonical SMILES | C[C@H](CCC(O)=O)[C@@]1([H])CC[C@@]2([H])[C@]3([H])[C@H](O)[C@@H](O)[C@]4([H])C([2H])([2H])[C@H](O)C([2H])([2H])C[C@]4(C)[C@@]3([H])CC[C@@]21C | ||
| 分子式 | C24H36D4O5 | 分子量 | 412.6 |
| 溶解度 | DMF: 30 mg/ml,DMF:PBS (pH 7.2) (1:1): 0.5 mg/ml,DMSO: 20 mg/ml,Ethanol: 20 mg/ml | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4237 mL | 12.1183 mL | 24.2365 mL |
| 5 mM | 0.4847 mL | 2.4237 mL | 4.8473 mL |
| 10 mM | 0.2424 mL | 1.2118 mL | 2.4237 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。