7,8-dihydro-L-Biopterin
(Synonyms: 7,8-二氢生物蝶呤) 目录号 : GC10533
A precursor in the synthesis of BH4
Cas No.:6779-87-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
7,8-dihydro-l-biopterin (BH2), an analogue of the natural cofactor BH4, is a precursor in the synthesis of BH4 [1].
Tetrahydrobiopterin (BH4) is a key redox-active cofactor involved in endothelial isoform of NO synthase (eNOS) catalysis. BH4 is an important determinant of NO-dependent signaling pathways. Oxidation of BH4 has been observed in vascular cells in the setting of the oxidative stress associated with diabetes [1,2].
In cultured aortic endothelial cells, supplementation with BH2 abolished VEGF-induced NO production. DHFR but not GTPCH1 knockdown increased reactive oxygen species (ROS) production. BH2 abolished the increase in ROS production induced by DHFR knockdown. Intracellular BH2, as well as the relative concentrations of BH4 and BH2, together play a determining role in the redox regulation of eNOS-modulated endothelial responses [2]. 7,8-dihydro-L-biopterin was a reduced form of pterins. Pterins noncompetitively inhibited rat liver GTP cyclohydrolase I activity. 7,8-dihydro-L-biopterin exhibited approximately 12-times more potent than oxidized pterins. The Ki values for 7,8-dihydro-L-biopterin was 14.4 μM [1].
References:
[1] Shen R, Alam A, Zhang Y. Inhibition of GTP cyclohydrolase I by pterins[J]. Biochimica et Biophysica Acta (BBA)-General Subjects, 1988, 965(1): 9-15.
[2] Sugiyama T, Levy B D, Michel T. Tetrahydrobiopterin recycling, a key determinant of endothelial nitric-oxide synthase-dependent signaling pathways in cultured vascular endothelial cells[J]. Journal of Biological Chemistry, 2009, 284(19): 12691-12700.
| Cas No. | 6779-87-9 | SDF | |
| 别名 | 7,8-二氢生物蝶呤 | ||
| 化学名 | 2-amino-6-((1R,2S)-1,2-dihydroxypropyl)-7,8-dihydropteridin-4(1H)-one | ||
| Canonical SMILES | NC(N(C1=C2N=C(CN1[H])[C@H]([C@H](C)O)O)[H])=NC2=O | ||
| 分子式 | C9H13N5O3 | 分子量 | 239.23 |
| 溶解度 | Water: Sparingly soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 4.1801 mL | 20.9004 mL | 41.8008 mL |
| 5 mM | 0.836 mL | 4.1801 mL | 8.3602 mL |
| 10 mM | 0.418 mL | 2.09 mL | 4.1801 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。