(Z)-10-Hydroxynortriptyline
(Synonyms: Z-10-羟基去甲替林) 目录号 : GC60427An active metabolite of amitriptyline and nortriptyline
Cas No.:47132-19-4
Sample solution is provided at 25 µL, 10mM.
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cis-10-hydroxy Nortriptyline is an active metabolite of the tricyclic antidepressants amitriptyline and nortriptyline .1,2 It is formed from amitriptyline and nortriptyline by the cytochrome P450 (CYP) isoform CYP2D6.1 cis-10-hydroxy Nortriptyline inhibits norepinephrine reuptake in rat cortical slices (EC50 = 160 nM).2
1.Coutts, R.T., Bach, M.V., and Baker, G.B.Metabolism of amitriptyline with CYP2D6 expressed in a human cell lineXenobiotica27(1)33-47(1997) 2.Bertilsson, l., Mellstr?m, B., and Sj?qvist, F.Pronounced inhibition of noradrenaline uptake by 10-hydroxymetabolites of nortriptylineLife Sci.25(15)1285-1292(1979)
Cas No. | 47132-19-4 | SDF | |
别名 | Z-10-羟基去甲替林 | ||
Canonical SMILES | OC1C2=CC=CC=C2/C(C3=CC=CC=C3C1)=C\CCNC.[(Z)] | ||
分子式 | C19H21NO | 分子量 | 279.38 |
溶解度 | 储存条件 | Store at -20°C | |
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1 mg | 5 mg | 10 mg | |
1 mM | 3.5794 mL | 17.8968 mL | 35.7935 mL |
5 mM | 0.7159 mL | 3.5794 mL | 7.1587 mL |
10 mM | 0.3579 mL | 1.7897 mL | 3.5794 mL |
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2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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Amitriptyline metabolism in relation to antidepressive effect
Clin Pharmacol Ther 1984 Apr;35(4):467-73.PMID:6705444DOI:10.1038/clpt.1984.61.
The relationship between amitriptyline (AT) metabolism and clinical response was studied in 14 outpatients treated with a daily dose of 150 mg AT. Riboflavin was added to the medication to check compliance. On days 0, 2, and 7 and at 3, 6, 9, and 13 wk after onset of therapy, blood samples were drawn from the patients 3 (+/- 0.5) hr after the first morning dose and a sample of the first morning urine was taken to check riboflavin. Serum levels of AT and its metabolites, nortriptyline (NT), E- and Z-10-hydroxynortriptyline (E- and Z-10-OH-NT), total (E + Z) 10-hydroxyamitriptyline (tot-10-OH-AT), and desmethylnortriptyline (DNT), were measured by means of HPLC while minimizing adsorption onto glass. On day 0 and after 6 and 13 wk the severity of the depressive disorder was scored by means of the self-rating depression scale of Zung.28 Mean steady-state concentrations of AT, NT, and E-10-OH-NT were in the order of 100 micrograms/l and tot-10-OH-AT and Z-10-OH-NT approximated 20 micrograms/l. DNT concentrations were under 15 micrograms/l. There was great variation in metabolic pattern between patients. After 6 wk concentrations of all compounds were approximately 15% lower than at 3 wk, indicating a weak autoinducible effect of AT or its metabolites. Steady-state concentrations of AT correlated well with that of NT (r = 0.64; P less than 0.05) but not with that of E-10-OH-NT.(ABSTRACT TRUNCATED AT 250 WORDS)
Comparison of amitriptyline metabolism in hepatocytes from streptozocin-induced diabetic rats and from non-diabetic rats
Cell Biol Toxicol 1997 Feb;13(2):131-7.PMID:9049103DOI:10.1023/b:cbto.0000010398.63757.4c.
Biotransformation of amitriptyline (AMI) was studied at different intervals in freshly isolated hepatocytes from healthy or streptozocin-induced diabetic rats in order to investigate the influence of the diabetic state. Levels of free and conjugated AMI, demethylated and hydroxylated metabolites, were assessed by HPLC analysis. In hepatocytes isolated from diabetic rats, AMI was less completely metabolized and the demethylation reaction became more important than in non-diabetic rat hepatocytes. Although the proportions of hydroxylated metabolites decreased in diabetic rats, it always remained predominant. Furthermore, glucuronidation of metabolites was greater, especially for (Z)-10-Hydroxynortriptyline in diabetic animals.