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TPPP Human

目录号 : GP24867

Tubulin Polymerization Promoting Protein Human Recombinant

TPPP Human Chemical Structure

规格 价格 库存 购买数量
2μg
¥840.00
5-10工作日
10μg
¥2,030.00
5-10工作日
1mg
¥72,800.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

Description

TPPP Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 229 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 24.9kDa (calculated).

Product Data

Purity Greater than 85.0% as determined by SDS-PAGE. Source Escherichia Coli.
Phycical Appearance Filtered White lyophilized (freeze-dried) powder. Shipping Condition Shipped at Room temp.
Synonyms Tubulin polymerization-promoting protein; TPPP; 25 kDa brain-specific protein; TPPP/p25; p24; p25-alpha; TPPP1; p25; p25alpha.
Amino Acid Sequence MKHHHHHHASMADKAKPAKA ANRTPPKSPG DPSKDRAAKR LSLESEGAGE GAAASPELSA LEEAFRRFAV HGDARATGRE MHGKNWSKLC KDCQVIDGRN VTVTDVDIVF SKIKGKSCRT ITFEQFQEAL EELAKKRFKD KSSEEAVREV HRLIEGKAPI ISGVTKAISS PTVSRLTDTT KFTGSHKERF DPSGKGKGKA GRVDLVDESG YVSGYKHAGT YDQKVQGGK.
Solubility It is recommended to add 200?l deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. TPPP is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Stability Store lyophilized protein at -20°C . Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C .
Formulation TPPP filtered (0.4 µm) and lyophilized from 0.5mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4.

Introduction

Tubulin Polymerization Promoting Protein (TPPP) probably plays a part in the polymerization of tubulin into microtubules, as well as in microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. TPPP may also have a role in mitotic spindle assembly and nuclear envelope breakdown. TPPP/p235 level in cerebrospinal fluid is considerably higher in multiple sclerosis patients.

Stability

Store lyophilized protein at -20°C . Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C .

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