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TNF a Human, His

目录号 : GP20965

Tumor Necrosis Factor-Alpha Human Recombinant, His Tag

TNF a Human, His Chemical Structure

规格 价格 库存 购买数量
10μg
¥840.00
5-10工作日
50μg
¥2,030.00
5-10工作日
1mg
¥21,000.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

产品文档

Quality Control & SDS

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产品描述

Tumor Necrosis Factor-a Human Recombinant His produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids fragment (77-233) and having a molecular mass of 18.3kDa with an amino-terminal hexahistidine tag. The TNF-alpha His is purified by standard chromatographic techniques.

Product Data

Purity Greater than 97.0% as determined by SDS-PAGE. Source Escherichia Coli.
Phycical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Shipping Condition Shipped at Room temp.
Synonyms TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; DIF; TNFA; TNFSF2.
Solubility It is recommended to reconstitute the lyophilized TNF-ж┴ in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized TNF-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Biological Activity EC50 0.200-0.120ng/ml measured in a metabolic inhibitor actinomycin D assay using L-cells (ATCC CCL-1).
Formulation TNF-a His is supplied in 1xPBS.

Introduction

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells.Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.

Biological Activity

EC50 0.200-0.120ng/ml measured in a metabolic inhibitor actinomycin D assay using L-cells (ATCC CCL-1).

Stability

Lyophilized TNF-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.