Timosaponin A1
(Synonyms: 知母皂苷A1) 目录号 : GC30611
A steroidal saponin with diverse biological activities
Cas No.:68422-00-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Timosaponin AI is a steroidal saponin that has been found in A. asphodeloides and has diverse biological activities.1,2,3 It is also a metabolite of timosaponin AIII .4 Timosaponin AI inhibits 5-lipoxygenase (5-LO), COX-2, and dipeptidyl peptidase 4 (DPP-4; IC50s = 3.29, 36.43, and 33.25 ?M, respectively).1,2 It reduces total cholesterol and triglyceride levels in 3T3-L1 differentiated adipocytes and increases glucose consumption in insulin-resistant BNL CL.2 mouse embryonic liver cells stimulated with insulin.2 Timosaponin AI is cytotoxic to NRK rat kidney epithelial and MOLM-13 acute myeloid leukemia (AML) cells (EC50s = 0.12 and 0.22 mM, respectively).3
1.Xie, L., Lee, D.Y.-W., Shang, Y., et al.Characterization of spirostanol glycosides and furostanol glycosides from anemarrhenae rhizoma as dual targeted inhibitors of 5-lipoxygenase and Cyclooxygenase-2 by employing a combination of affinity ultrafiltration and HPLC/MSPhytomedicine77153284(2020) 2.Jiang, S., Wu, X., Wang, Y., et al.The potential DPP-4 inhibitors from Xiao-Ke-An improve the glucolipid metabolism via the activation of AKT/GSK-3β pathwayEur. J. Pharmacol.882173272(2020) 3.Carpinteyro Díaz, A.E., Herfindal, L., Rathe, B.A., et al.Cytotoxic saponins and other natural products from flowering tops of Narthecium ossifragum LPhytochemistry16467-77(2019) 4.Sun, Y., Liu, L., Peng, Y., et al.Metabolites characterization of timosaponin AIII in vivo and in vitro by using liquid chromatography-mass spectrometryJ. Chromatogr. B Analyt. Technol. Biomed. Life Sci.997236-243(2015)
| Cas No. | 68422-00-4 | SDF | |
| 别名 | 知母皂苷A1 | ||
| Canonical SMILES | C[C@@]12[C@]3([H])[C@](O[C@]4(CC[C@H](C)CO4)[C@H]3C)([H])C[C@@]1([H])[C@@]5([H])[C@]([C@@]6([C@](C[C@@H](O[C@]7([H])O[C@@H]([C@H](O)[C@H](O)[C@H]7O)CO)CC6)([H])CC5)C)([H])CC2 | ||
| 分子式 | C33H54O8 | 分子量 | 578.78 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7278 mL | 8.6389 mL | 17.2777 mL |
| 5 mM | 0.3456 mL | 1.7278 mL | 3.4555 mL |
| 10 mM | 0.1728 mL | 0.8639 mL | 1.7278 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。