T-PA Protein, Mouse (HEK293, His)
目录号 : GC26822T-PA 蛋白通过水解单个 Arg-Val 键将无活性的纤溶酶原转化为活性纤溶酶。这种转化调节纤溶酶介导的蛋白水解，并参与组织重塑、降解和细胞迁移。
Sample solution is provided at 25 µL, 10mM.
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T-PA 蛋白通过水解单个 Arg-Val 键将无活性的纤溶酶原转化为活性纤溶酶。这种转化调节纤溶酶介导的蛋白水解，并参与组织重塑、降解和细胞迁移。T-PA 还通过参与皮质颗粒反应，有助于在卵母细胞激活过程中预防多精受精。T-PA 蛋白, Mouse (HEK293, His) 是重组的 T-PA 蛋白，由 HEK293 表达，带有 C-His 标签。
T-PA 蛋白通过单个 Arg-Val 键的水解将无活性的酶原纤溶酶原转化为活性纤溶酶来发挥作用。这种转化对于调节纤溶酶介导的蛋白水解至关重要，蛋白水解参与组织重塑、降解、细胞迁移以及各种生理和病理过程。此外，在卵母细胞激活过程中，T-PA 在透明带反应内的皮质颗粒反应中发挥作用，有助于预防多精受精。
| Purity | Greater than 95% as determined by Tris-Bis PAGE. | Source | HEK293 |
| Phycical Appearance | Lyophilized powder | Shipping Condition | |
| Synonyms | T-PA 蛋白; Tissue-type plasminogen activator; t-PA; Plat | ||
| Amino Acid Sequence | SYRATCRDEPTQTTYQQHQSWLRPMLRSSRVEYCRCNSGLVQCHSVPVRSCSEPRCFNGGTCQQALYFSDFVCQCPDGFVGKRCDIDTRATCFEEQGITYRGTWSTAESGAECINWNSSVLSLKPYNARRPNAIKLGLGNHNYCRNPDRDLKPWCYVFKAGKYTTEFCSTPACPKGKSEDCYVGKGVTYRGTHSLTTSQASCLPWNSIVLMGKSYTAWRTNSQALGLGRHNYCRNPDGDARPWCHVMKDRKLTWEYCDMSPCSTCGLRQYKRPQFRIKGGLYTDITSHPWQAAIFVKNKRSPGERFLCGGVLISSCWVLSAAHCFLERFPPNHLKVVLGRTYRVVPGEEEQTFEIEKYIVHEEFDDDTYDNDIALLQLRSQSKQCAQESSSVGTACLPDPNLQLPDWTECELSGYGKHEASSPFFSDRLKEAHVRLYPSSRCTSQHLFNKTVTNNMLCAGDTRSGGNQDLHDACQGDSGGPLVCMINKQMTLTGIISWGLGCGQKDVPGVYTKVTNYLDWIHDNMKQ | ||
| Apparent Molecular Weight | 13-15 kDa & 30-34 kDa & 35-40 kDa | ||
| Stability | Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | ||
| Biological Activity | Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is > 500 pmol/min/μg. | ||
| Endotoxin Level | <1 EU/μg, determined by LAL method. | ||
| Reconstitution | It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose). | ||
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. | ||
The T-PA protein converts the inactive plasminogen to active plasmin by hydrolyzing a single Arg-Val bond.This conversion regulates plasmin-mediated proteolysis and is involved in tissue remodeling, degradation, and cell migration.T-PA also contributes to the prevention of polyspermy during oocyte activation by participating in the cortical granule reaction.T-PA Protein, Mouse (HEK293, His) is the recombinant mouse-derived T-PA protein, expressed by HEK293 , with C-His labeled tag.
Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is > 500 pmol/min/μg.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.