Sudan Red 7B
(Synonyms: 苏丹红7B) 目录号 : GC48625
An azo dye
Cas No.:6368-72-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Sudan red 7B is an azo dye.1 It displays an absorption maxima of 525 nm.2 Sudan red 7B has been used to stain lipids in hepatocytes and suberin lamellae in plant cell walls.3,4 It has also been included as a standard in LC-MS/MS-based protocols for the detection of banned colorants in foodstuffs.1
1.Pardo, O., YusÀ, V., LeÓn, N., et al.Development of a method for the analysis of seven banned azo-dyes in chilli and hot chilli food samples by pressurised liquid extraction and liquid chromatography with electrospray ionization-tandem mass spectrometryTalanta78(1)178-186(2009) 2.Nagase, M., Matsueda, T., and Osaki, Y.Determination of Sudan III, Sudan IV and Sudan red 7B in water by high performance liquid chromatography after mixing extractionAnal. Sci.5(2)157-160(1989) 3.Delzenne, N.M., Hernaux, N.A., and Taper, H.S.A new model of acute liver steatosis induced in rats by fasting followed by refeeding a high carbohydrate-fat free diet. Biochemical and morphological analysisJ. Hepatol.26(4)880-885(1997) 4.Brundrett, M.C., Kendrick, B., and Peterson, C.A.Efficient lipid staining in plant material with Sudan red 7B or fluorol [correction of fluoral] yellow 088 in polyethylene glycol-glycerolBiotech. Histochem.66(3)111-116(1991)
| Cas No. | 6368-72-5 | SDF | |
| 别名 | 苏丹红7B | ||
| Canonical SMILES | CCNC(C=CC1=C2C=CC=C1)=C2N=NC(C=C3)=CC=C3N=NC4=CC=CC=C4 | ||
| 分子式 | C24H21N5 | 分子量 | 379.5 |
| 溶解度 | DMF: 30 mg/ml,DMSO: 30 mg/ml,DMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml,Ethanol: 1 mg/ml | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.635 mL | 13.1752 mL | 26.3505 mL |
| 5 mM | 0.527 mL | 2.635 mL | 5.2701 mL |
| 10 mM | 0.2635 mL | 1.3175 mL | 2.635 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
UPLC-ESI-MS/MS analysis of Sudan dyes and Para Red in food
Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2010 Sep;27(9):1215-20.PMID:20582780DOI:10.1080/19440049.2010.483600.
An analytical method for the simultaneous determination of Sudan dyes (Sudan Red G, Sudan I, Sudan II, Sudan III, Sudan Red 7B and Sudan IV) and Para Red in food by ultra-performance liquid chromatography-electrospray tandem mass spectrometry (UPLC-ESI-MS/MS) was developed. Samples were extracted with acetonitrile, and water added into the extract. The supernatant was analysed by UPLC-MS/MS after refrigeration and centrifugation. The sample was separated on an Acquity BEH C(18) column, and detected by MS/MS with the multiple reaction monitoring mode. Matrix calibration was used for quantitative testing of the method. The linear matrix calibrations of Sudan dyes and Para Red were 2-50 and 10-250 ng g(-1), respectively, and the regression coefficients were >0.9945. The recoveries were 83.4-112.3% with good coefficients of variation of 2.0-10.8%. The limits of detection were between 0.3 and 1.4 ng g(-1) for the six Sudan dyes, and between 3.7 and 6.0 ng g(-1) for Para Red. The limits of quantification were between 0.9 and 4.8 ng g(-1) for the six Sudan dyes, and between 12.2 and 19.8 ng g(-1) for Para Red.
Efficient lipid staining in plant material with Sudan Red 7B or fluorol [correction of fluoral] yellow 088 in polyethylene glycol-glycerol
Biotech Histochem 1991;66(3):111-6.PMID:1716161DOI:10.3109/10520299109110562.
Polyethylene glycol (400) with 90% glycerol (aqueous) is introduced as an efficient solvent system for lipid stains. Various lipid-soluble dyes were dissolved in this solvent system and tested for their intensity, contrast, and specificity of staining of suberin lamellae in plant tissue. The stability (i.e., lack of precipitation) of the various staining solutions in the presence of fresh tissue was also tested. When dissolved in polyethylene glycol-glycerol, Sudan Red 7B (fat red) was the best nonfluorescent stain and fluorol yellow 088 (solvent green 4) was an excellent fluorochrome. These two dyes formed stable staining solutions which efficiently stained lipids in fresh sections without forming precipitates. Estimations of the solubilities of these dyes in the solvent compared with their solubilities in lipids of various chemical types indicated that they should both be effective stains for lipids in general.
γ-Fe2O3 magnetic nanoparticle functionalized with carboxylated multi walled carbon nanotube for magnetic solid phase extractions and determinations of Sudan dyes and Para Red in food samples
Food Chem 2018 Mar 1;242:533-537.PMID:29037726DOI:10.1016/j.foodchem.2017.09.039.
Hybrid nanostructures composed of γ-Fe2O3 (maghemite) and carboxylated-multi walled carbon nanotube (cMWCNT) were used for the magnetic solid phase extractions and determination of Sudan I, II, III, IV, Para Red, Sudan Black B and Sudan Red 7B in chili products. High performance liquid chromatography (HPLC) was employed for the measurements. Limit of quantification (LOQ) values were found in the range 0.44-2.82ngmL-1 for analytes. The best extraction parameters were determined as pH 8.0, 40mg of magnetic nanoparticle, 4.0min of contact time, 0.3mL desorption by acetonitrile. The samples were dissolved in acetone-dichloromethane-methanol (3:2:1, v/v/v) and diluted with acetonitrile-methanol (v/v; 80:20) before the method was applied. Concentrations of Sudan dyes and Para Red were determined in four samples of chili powder from less than LOQ to 31.21±1.6ngg-1, two samples of chili tomato sauces (lower than LOQ) and two samples of ketchup (lower than LOQ).
Determination of 20 synthetic dyes in chili powders and syrup-preserved fruits by liquid chromatography/tandem mass spectrometry
J Food Drug Anal 2015 Sep;23(3):453-462.PMID:28911703DOI:10.1016/j.jfda.2014.09.003.
A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method is developed to simultaneously determine 20 synthetic dyes (New Coccine, Indigo Carmine, Erythrosine, Tartrazine, Sunset Yellow FCF, Fast Green FCF, Brilliant Blue FCF, Allura Red AC, Amaranth, Dimethyl Yellow, Fast Garnet GBC, Para Red, Sudan I, Sudan II, Sudan III, Sudan IV, Sudan Orange G, Sudan Red 7B, Sudan Red B, and Sudan Red G) in food samples. This method offers high sensitivity and selectivity through the selection of two fragment ion transitions under multiple reaction monitoring mode to satisfy the requirements of both quantitation and qualitation. Using LC-MS/MS, the newly developed extraction protocol used in this study is rapid and simple and does not require the use of solid-phase extraction cartridges. The linearities and recoveries of the method are observed at the concentration range of 0.10-200 μg/kg and more than 90% for all dyes, respectively. The method has been successfully applied to screen 18 commercial chili powders and six commercial syrup-preserved fruits purchased from retail establishments in Taipei City. The results show that three legal food dyes, Tartrazine, and/or Sunset Yellow FCF, and/or New Coccine, are present in some syrup-preserved fruits. Amaranth, an illegal food dye in certain countries but declared illegal in Taiwan, is found in an imported syrup-preserved fruit.
Oil-soluble dyes for marking Spodoptera frugiperda (Lepidoptera: Noctuidae)
J Econ Entomol 2006 Dec;99(6):2110-5.PMID:17195680DOI:10.1603/0022-0493-99.6.2110.
Although various biological aspects of Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) have been examined, adult movement and dispersal of this insect pest is not well understood. Release-recapture techniques by using marked insects is a useful approach for dispersal studies; however, the marking technique should not significantly affect insect biology or behavior. Therefore, the effect of different concentrations of oil-soluble dyes (Solvent Blue 35 [C.I. 61554], Sudan Red 7B [C.I. 26050], Sudan Black B [26150], Sudan Orange G [C.I. 11920], and Sudan I 103624 [C.I. 12055]) on development, mortality, and fecundity of S. frugiperda was evaluated. Dyes were added to artificial diet used to feed larvae. Larval and pupal development and mortality, adult longevity, and female fecundity were evaluated. High concentrations (400 and 600 ppm) of all dyes led to longer larval and pupal stages. Adult life span and number of eggs were not affected by the dyes. Sudan Red 7B marked both adults and eggs very well. Solvent Blue 35 marked both adults and eggs, but the blue-marked eggs could not be distinguished from some bluish eggs laid by nonlabeled females. Adults and eggs were not adequately marked by the Sudan Black B, Sudan Orange G, and Sudan I 103624 (a yellow dye).