Streptolysin-O, His
目录号 : GP24735Streptococcus Pyogenes Streptolysin-O Recombinant, His Tag
Sample solution is provided at 25 µL, 10mM.
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Recombinant Streptolysin-O produced in E.coli is a non-glycosylated, polypeptide chain having a calculated molecular mass of 61kDa.Streptolysin-O is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
| Purity | Greater than 80.0% as determined by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered clear solution. | Shipping Condition | Shipped with Ice Packs. |
| Synonyms | Streptolysin O; Thiol-activated cytolysin; slo. | ||
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time.Avoid multiple freeze-thaw cycles. | ||
| Formulation | Streptolysin-O is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol. | ||
Streptolysin-O is a sulfhydryl-activated toxin which causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the Streptolysin-O protein undergoes a major conformation change, leading to its insertion in the host membrane and creation of an oligomeric pore complex. Cholesterol may be needed for binding to host membranes, membrane insertion and pore formation. Streptolysin-O can be reversibly inactivated by oxidation.
Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time.Avoid multiple freeze-thaw cycles.