StemRegenin 1 (hydrochloride)
(Synonyms: SR1) 目录号 : GC17248
Antagonizes hematopoietic stem cell differentiation
Cas No.:2319882-01-2
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
IC50: 127 nM in CD34+ cells
StemRegenin 1 is an aryl hydrocarbon receptor signaling antagonist.
The aryl hydrocarbon receptor (AhR ) is a protein that is encoded by the AHR gene. The AhR is a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic (aryl) hydrocarbons.
In vitro: In previous study, the unbiased screen with primary human HSCs identified the StemRegenin 1 (SR1), which could promote the ex-vivo expansion of CD34+ cells. In addition, the culture of HSCs with SR1 resulted in a 50-fold increase in cells expressing CD34. Moreover, the mechanistic studies showed that SR1 acted by antagonizing the AhR [1].
In vivo: In aninmal study, 300 to 30,000 uncultured CB CD34+ cells were transplanted into NSG mice. Results showed that CB CD34+ cells cultured with SR1 led to significant increases in human engraftment in the peripheral blood at 1 week, compared with mice transplanted with 10,000 uncultured cells or the progeny of 10,000 control cells [1].
Clinical trial: An open label, randomized phase II trial comparing SR-1 cultured umbilical cord blood to unmanipulated umbilical cord blood transplantation after a myeloablative CY/FLU/TBI conditioning has been proposed, but is not yet open for participant recruitment [2].
References:
[1] A. E. Boitano, J. Wang, R. Romeo, et al. Aryl hydrocarbon receptor antagonists promote the expansion of human hematopoietic stem cells. Science 329,1345-1348 (2010).
[2] https://clinicaltrials. gov/ct2/show/NCT02765997 term=StemRegenin+1&rank=1
| Cas No. | 2319882-01-2 | SDF | |
| 别名 | SR1 | ||
| 化学名 | 4-(2-((2-(benzo[b]thiophen-3-yl)-9-isopropyl-9H-purin-6-yl)amino)ethyl)phenol, monohydrochloride | ||
| Canonical SMILES | OC1=CC=C(C=C1)CCNC2=C3C(N(C(C)C)C=N3)=NC(C4=CSC5=C4C=CC=C5)=N2.Cl | ||
| 分子式 | C24H23N5OS • HCl | 分子量 | 466.0 |
| 溶解度 | ≤5mg/ml in DMSO;5mg/ml in dimethyl formamide | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.1459 mL | 10.7296 mL | 21.4592 mL |
| 5 mM | 0.4292 mL | 2.1459 mL | 4.2918 mL |
| 10 mM | 0.2146 mL | 1.073 mL | 2.1459 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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