SR 0987
目录号 : GC14054
An agonist of RORγt
Cas No.:303126-97-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
SR 0987 is an agonist of RORγt with EC50 value of ~800 nM for inducing reporter gene expression [1].
RORγt (retinoic acid receptor-related orphan receptor-γt), the T cell-specific isoform of RORγ, is the key lineage-defining transcription factor to initiate the differentiation program of TH17 and Tc17 cells, cells that have demonstrated anti-tumor efficacy [1].
SR 0987 is a RORγt agonist. In murine EL4 T lymphocytes, SR 0987 increased expression of IL17 while repressing the expression of the checkpoint receptor PD-1, suggesting that SR 0987 enhanced protective immunity by regulating expression of IL17 and PD-1 while maintaining the cytotoxic ability of these cells. In both the 5xRORE and IL17 promoter transfected cells, SR0987 concentration dependently induced reporter gene expression with an EC50 of ~800 nM. SR0987 also concentration dependently increased interaction of RORγt with the SRC1-3 NR box peptide [1].
Reference:
[1]. Chang MR, Dharmarajan V, Doebelin C, et al. Synthetic RORγt Agonists Enhance Protective Immunity. ACS Chem Biol. 2016 Apr 15;11(4):1012-8.
| Cas No. | 303126-97-8 | SDF | |
| 化学名 | 2-chloro-N-[4-[2,2,2-trifluoro-1-hydroxy-1-(trifluoromethyl)ethyl]phenyl]-benzamide | ||
| Canonical SMILES | ClC1=CC=CC=C1C(NC2=CC=C(C(C(F)(F)F)(C(F)(F)F)O)C=C2)=O | ||
| 分子式 | C16H10ClF6NO2 | 分子量 | 397.7 |
| 溶解度 | ≤30mg/ml in ethanol;15mg/ml in DMSO;20mg/ml in dimethyl formamide | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.5145 mL | 12.5723 mL | 25.1446 mL |
| 5 mM | 0.5029 mL | 2.5145 mL | 5.0289 mL |
| 10 mM | 0.2514 mL | 1.2572 mL | 2.5145 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。