RS 09
目录号 : GC50297
RS 09是一种能够激活TLR-4信号通路的合成肽。RS 09可以作为佐剂增强BALB/c小鼠的免疫反应。
Cas No.:1449566-36-2
Sample solution is provided at 25 µL, 10mM.
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RS 09 is a synthetic peptide that can activate the TLR-4 signaling pathway. RS 09 can function as an adjuvant to enhance the immune response in BALB/c mice[1].
In vitro, RS 09 (10µM; 30min) rescued the suppressed protein levels of TLR4, p-p65/p65, and p-IκBα/IκBα caused by ETS2 deficiency in the inflammatory cell model[2]. The TLR4 agonist RS 09 (5μg/ml; 24h) impaired the neuroprotective effects of corilagin on N2a cells[3]. RS 09 (60µM) intervention inhibited anti-inflammatory and pro-M2 polarizing effects of metformin, activating TLR4/NF-κB pathway[4].
In vivo, In BALB/c mice, when used in combination with the prostate cancer-specific antigen X-15, RS 09 (25µg; 28 days; s.c.) significantly increased the levels of X-15-specific antibodies[1].
References:
[1] Shanmugam A, Rajoria S, George AL, et al. Synthetic Toll like receptor-4 (TLR-4) agonist peptides as a novel class of adjuvants. PLoS One. 2012;7(2):e30839.
[2] Liu B, Yu J, Zhang J, et al. TLR4/NF-κB-mediated M1 macrophage polarization contributes to the promotive effects of ETS2 on ulcerative colitis. Eur J Med Res. 2025 Jul 24;30(1):668.
[3] Lei Y, Zhou J, Xu D, et al. Corilagin Attenuates Neuronal Apoptosis and Ferroptosis of Parkinson's Disease through Regulating the TLR4/Src/NOX2 Signaling Pathway. ACS Chem Neurosci. 2025 Mar 5;16(5):968-980.
[4] Shen W, Wang Q, Shen G, et al. Regulation of macrophage polarization by metformin through inhibition of TLR4/NF-κB pathway to improve pre-eclampsia. Placenta. 2025 Feb;160:89-99.
RS 09是一种能够激活TLR-4信号通路的合成肽。RS 09可以作为佐剂增强BALB/c小鼠的免疫反应[1]。
在体外实验中，RS 09（10µM; 30分钟）能够恢复由于ETS2缺失导致的炎症细胞模型中TLR4、p-p65/p65和p-IκBα/IκBα蛋白水平的抑制[2]。TLR4激动剂RS 09（5µg/ml; 24小时）损害了诃子酸对N2a细胞的神经保护作用[3]。RS 09（60µM）干预抑制了二甲双胍的抗炎和促M2极化作用，激活了TLR4/NF-κB信号通路[4]。
在体内实验中，在BALB/c小鼠中，当与前列腺癌特异性抗原X-15联合使用时，RS 09（25µg; 28天; 皮下注射）显著增加了X-15特异性抗体的水平[1]。
| Cell experiment [1]: | |
Cell lines | RAW264.7 cells |
Preparation Method | The sh-NC/sh-ETS2 lentivirus was transfected into mouse macrophage RAW264.7 cells for 48h to achieve stable silencing of ETS2. To induce M1 macrophage polarization and establish an inflammatory cell model, the cells were stimulated with lipopolysaccharide (LPS) and interferon-γ (IFN-γ) for 12h. Subsequently, the TLR4 agonist RS 09 (10µM) was added to the cells for 30min to activate the TLR4/NF-κB signaling pathway. The RAW264.7 cells were then collected and resuspended in PBS. Next, 3µL of PE-conjugated iNOS and FITC-conjugated F4/80 antibodies were added, and the cells were incubated in the dark for 20-30min. After another wash, the cells were resuspended in 200µL of PBS, and the proportion of the iNOS+/F4/80+ subgroup was monitored by flow cytometry. |
Reaction Conditions | 10µM; 30min |
Applications | RS 09 rescued the suppressed protein levels of TLR4, p-p65/p65, and p-IκBα/IκBα caused by ETS2 deficiency in the inflammatory cell model. |
| Animal experiment [2]: | |
Animal models | Eight-week-old male BALB/c mice |
Preparation Method | Eight-week-old male BALB/c mice were divided into four groups (three mice per group). The 15-mer peptide antigen (X-15 peptide) was conjugated to Keyhole Limpet Hemocyanin (KLH) and injected subcutaneously. All mice were vaccinated with 100µg of X-15 conjugated to KLH on day 0 and received a booster of 50µg of X-15 conjugated to KLH on day 14. The difference between the groups was the type of adjuvant used, which was either Alum, RS 01, or RS 09. Alum was added to X-15-KLH at a 1:1 (v:v) ratio, while both RS 01 and RS 09 were added at a concentration of 25µg. OVA peptide (an 8-mer peptide) served as a negative non-adjuvant peptide control and was also used at a concentration of 25µg. Blood was collected on days 0, 14, and 28. The serum collected was used to determine the presence of X-15-specific antibodies via ELISA. |
Dosage form | 25µg; 28 days; s.c. |
Applications | In BALB/c mice, when used in combination with the prostate cancer-specific antigen X-15, RS 09 significantly increased the levels of X-15-specific antibodies. |
References: | |
| Cas No. | 1449566-36-2 | SDF | |
| Canonical SMILES | [H]N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(O)=O | ||
| 分子式 | C31H49N9O9 | 分子量 | 691.78 |
| 溶解度 | Water : 100 mg/mL (144.55 mM; Need ultrasonic) | 储存条件 | Store at -20°C, Sealed, light and moisture resistant storage |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.4455 mL | 7.2277 mL | 14.4555 mL |
| 5 mM | 289.1 μL | 1.4455 mL | 2.8911 mL |
| 10 mM | 144.6 μL | 722.8 μL | 1.4455 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet