Resorufin sodium salt (NSC 12097 sodium salt)
(Synonyms: 试卤灵钠盐; NSC 12097 sodium salt) 目录号 : GC33591
A fluorescent probe
Cas No.:34994-50-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Resorufin is a fluorescent probe and the reduced form of the redox indicator dye resazurin .1 It can be further reduced to hydroresorufin, which is non-fluorescent. Resorufin has been used in the synthesis of resorufin-based fluorescent probes.2,3 It has also been used for the direct measurement of solvent-solute hydrogen bond dynamics with distinct absorption and emission peaks in solvents with strong hydrogen bonds and overlapping peaks in solvents with weak hydrogen bonds.4 It displays excitation/emission maxima of 570/580 nm, respectively.
1.O'Brien, J., Wilson, I., Orton, T., et al.Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicityEur. J. Biochem.267(17)5421-5426(2000) 2.Mayer, R.T., Jermyn, J.W., Burke, M.D., et al.Methoxyresorufin as a substrate for the fluorometric assay of insect microsomal O-dealkylasesPestic. Biochem. Physiol.7(4)349-354(1977) 3.Yoon, J.W., Kim, S., Yoon, Y., et al.A resorufin-based fluorescent turn-on probe responsive to nitroreductase activity and its application to bacterial detectionDyes Pigm.171107779(2019) 4.Yu, J., and Berg, M.Resorufin as a probe for the dynamics of solvation by hydrogen bondingChem. Phys. Lett.208(3-4)315-320(1993)
| Cas No. | 34994-50-8 | SDF | |
| 别名 | 试卤灵钠盐; NSC 12097 sodium salt | ||
| Canonical SMILES | O=C1C=CC2=NC3=C(OC2=C1)C=C(C=C3)O[Na] | ||
| 分子式 | C12H6NNaO3 | 分子量 | 235.17 |
| 溶解度 | Water : 10 mg/mL | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.2522 mL | 21.2612 mL | 42.5224 mL |
| 5 mM | 0.8504 mL | 4.2522 mL | 8.5045 mL |
| 10 mM | 0.4252 mL | 2.1261 mL | 4.2522 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。