Triclosan
(Synonyms: 三氯生) 目录号 : GC13311
Triclosan 是一种广谱抗菌剂。
Cas No.:3380-34-5
Sample solution is provided at 25 µL, 10mM.
Triclosan is a broad-spectrum antibacterial agent[1]. Triclosan inhibits bacterial fatty acid synthesis at the enoyl-acyl carrier protein reductase (FabI) step[2]. Triclosan is commonly used for antibacterial research and is widely found in daily necessities such as soaps, detergents, toys, and surgical cleaning treatments[3][4].
In vitro, Triclosan (1-100µM; 1-24h) decreased cell viability and induced cell apoptosis in a dose- and time-dependent manner in rat neural stem cells (NSCs)[5].
In vivo, Triclosan (50-200mg/kg/day; p.o.; 7 days) significantly reduced serum testosterone levels, downregulated the expression of Leydig cell genes involved in testosterone biosynthesis in adult male rats[6]. Triclosan (80ppm in diet; 4 weeks) increased the severity of dextran sodium sulfate (DSS)-induced colitis, and promoted colonic inflammation and immune cell infiltration in mice[7].
References:
[1] Vosatka R, Kratky M, Vinsova J. Triclosan and its derivatives as antimycobacterial active agents. Eur J Pharm Sci. 2018;114:318-331.
[2] Heath RJ, Rubin JR, Holland DR, Zhang E, Snow ME, Rock CO. Mechanism of triclosan inhibition of bacterial fatty acid synthesis. J Biol Chem. 1999;274(16):11110-11114.
[3] Shrestha P, Zhang Y, Chen WJ, Wong TY. Triclosan: antimicrobial mechanisms, antibiotics interactions, clinical applications, and human health. J Environ Sci Health C Toxicol Carcinog. 2020;38(3):245-268.
[4] Weatherly LM, Gosse JA. Triclosan exposure, transformation, and human health effects. J Toxicol Environ Health B Crit Rev. 2017;20(8):447-469.
[5] Park BK, Gonzales EL, Yang SM, Bang M, Choi CS, Shin CY. Effects of Triclosan on Neural Stem Cell Viability and Survival. Biomol Ther (Seoul). 2016;24(1):99-107.
[6] Sang J, Ji Z, Li H, et al. Triclosan inhibits testosterone biosynthesis in adult rats via inducing m6A methylation-mediated autophagy. Environ Int. 2024;190:108827.
[7] Zhang J, Walker ME, Sanidad KZ, et al. Microbial enzymes induce colitis by reactivating triclosan in the mouse gastrointestinal tract. Nat Commun. 2022;13(1):136.
Triclosan 是一种广谱抗菌剂[1]。Triclosan 抑制细菌脂肪酸在烯酰基载体蛋白还原酶 (FabI) 阶段的合成[2]。Triclosan 常用于抗菌研究，并广泛存在于肥皂、洗涤剂、玩具和外科清洁治疗等日用品中[3][4]。
体外实验中，Triclosan（1-100µM；1-24小时）以剂量和时间依赖性方式降低大鼠神经干细胞（NSCs）的细胞活力，并诱导细胞凋亡[5]。
体内实验中，Triclosan（50-200mg/kg/天；口服；7天）显著降低了成年雄性大鼠的血清睾酮水平，并下调了睾丸间质细胞中与睾酮生物合成相关的基因表达[6]。Triclosan（饮食中 80ppm；4周）加剧了小鼠葡聚糖硫酸钠（DSS）诱导的结肠炎，促进了结肠炎症和免疫细胞浸润[7]。
| Cell experiment [1]: | |
Cell lines | Neural stem cells |
Preparation Method | Neural stem cells were cultured from embryonic day 14 (E14) brain of Sprague-Dawley rat. In brief, cortices were dissociated into single cells by mechanical trituration, and cells were incubated in DMEM/F12 supplemented with B27-serum free supplement and growth factor (10ng/ml FGF and 20ng/ml EGF) in a 5% CO2 incubator. EGF and FGF were added every day and the cells grew into floating neurospheres, which were dissociated into single cells using trypsin-EDTA (0.1%) and plated into poly-L-ornithine pre-coated plates using B27- medium without growth factors for differentiation. Neural stem wells were treated in vitro with Triclosan at 1, 10, 20, 30, 50 and 100μM concentrations and applied into the culture media at different lengths of exposure (3, 6, 8, 12 and 24h) to determine the dose and time-dependent effects. All drug treatments and experiments were conducted during the 4th day of in vitro culture (DIV4) of NSCs. To evaluate neuronal cell death in culture Triclosan treatment, PI staining was performed. MTT assay was used to examine whether Triclosan treatment can influence NSC proliferation. |
Reaction Conditions | 1-100µM; 1-24h |
Applications | Triclosan decreased cell viability and induced cell apoptosis in a dose- and time-dependent manner in rat neural stem cells (NSCs). |
| Animal experiment [2]: | |
Animal models | Adult male Sprague-Dawley rats |
Preparation Method | 24 adult male Sprague-Dawley rats (56 days old) rats were housed in cages free from Triclosan following a one-week acclimatization period, under the conditions of temperature maintained at 23±2℃, humidity levels ranging from 45% to 55%, and a 12h-12h light-dark cycle. The rats were randomly divided into four groups consisting of 6 rats in each group: control group (corn oil), 50mg/kg/day, 100mg/kg/day, and 200mg/kg/day Triclosan groups. Each dose of Triclosan was mixed with 1ml of corn oil and administered to the rats at a rate of 1ml per kilogram of body weight. The administration regimen for the rats extended from postnatal day (PND) 56 to PND 63, a timeframe that aligns with the transition to adulthood. This period was selected to assess the potential effects of Triclosan exposure during a critical developmental window on subsequent hormonal regulation. The body weight of each rat was recorded throughout the study. At the end of the Triclosan exposure period, the rats were euthanized using CO2 followed by cervical dislocation. Trunk blood was collected, centrifuged at 1500×g for 10min to obtain serum. The testes and epididymis were removed and weighed. One testis was stored at -80℃ for mRNA and protein analysis, while the other testis was fixed in Bouin’s fixative for immunohistochemistry. Sperm counting was performed using the cauda epididymis. |
Dosage form | 50-200mg/kg/day; p.o.; 7 days |
Applications | Triclosan significantly reduced serum testosterone levels, downregulated the expression of Leydig cell genes involved in testosterone biosynthesis in adult male rats. |
References: | |
| Cas No. | 3380-34-5 | SDF | |
| 别名 | 三氯生 | ||
| 化学名 | 5-chloro-2-(2,4-dichlorophenoxy)-phenol | ||
| Canonical SMILES | ClC1=C(OC2=CC=C(Cl)C=C2O)C=CC(Cl)=C1 | ||
| 分子式 | C12H7Cl3O2 | 分子量 | 289.5 |
| 溶解度 | ≥ 10.95mg/mL in DMSO | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.4542 mL | 17.2712 mL | 34.5423 mL |
| 5 mM | 690.8 μL | 3.4542 mL | 6.9085 mL |
| 10 mM | 345.4 μL | 1.7271 mL | 3.4542 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet