PRL-3 Inhibitor
(Synonyms: BR-1,P0108,Phosphatase of Regenerating Liver 3 Inhibitor,PTP4A3 Inhibitor) 目录号 : GC16900PRL-3 Inhibitor是一种针对再生肝磷酸酶-3（PRL-3）的抑制剂，其半数抑制浓度（IC50）为0.9μM。
Cas No.:893449-38-2
Sample solution is provided at 25 µL, 10mM.
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The PRL-3 Inhibitor is an inhibitor of phosphatase of regenerating liver-3 (PRL-3) with an IC50 of 0.9μM[1].
In vitro, PRL-3 Inhibitor (compound 5e) (0, 1, 10, and 30μg/mL; 22h) inhibits the invasion of mouse melanoma B16F10 cells[1].
References:
[1] Ahn JH, Kim SJ, Park WS, et al. Synthesis and biological evaluation of rhodanine derivatives as PRL-3 inhibitors. Bioorg Med Chem Lett. 2006 Jun 1;16(11):2996-9.
PRL-3 Inhibitor是一种针对再生肝磷酸酶-3（PRL-3）的抑制剂，其半数抑制浓度（IC50）为0.9μM[1]。
在体外实验中，PRL-3 Inhibitor（compound 5e）（0, 1, 10和30μg/mL; 22小时）能够抑制小鼠黑色素瘤B16F10细胞的侵袭[1]。
| Cell experiment [1]: | |
Cell lines | B16F10 cells |
Preparation Method | A total of 1×10⁵ B16F10 cells were seeded in the upper chamber of 24-well invasion chambers with 8μm polycarbonate filters, while the lower chamber contained conditioned medium from HT1080 cells as a chemoattractant. The cells were incubated for 22h at 37℃ in a humidified incubator with 5% CO₂ in the presence and absence of PRL-3 Inhibitor (0, 1, 10, and 30μg/mL). Non-migratory cells on the upper surface of the filter were removed by wiping with a cotton swab. Migrated cells on the underside of the membrane were stained with 0.5% crystal violet after fixation with methanol and observed under a microscope. |
Reaction Conditions | 0, 1, 10, and 30μg/mL; 22h |
Applications | PRL-3 Inhibitor (compound 5e) inhibits the invasion of mouse melanoma B16F10 cells. |
References: | |
| Cas No. | 893449-38-2 | SDF | |
| 别名 | BR-1,P0108,Phosphatase of Regenerating Liver 3 Inhibitor,PTP4A3 Inhibitor | ||
| 化学名 | 5-[[5-bromo-2-[(2-bromophenyl)methoxy]phenyl]methylene]-2-thioxo-4-thiazolidinone | ||
| Canonical SMILES | BrC1=CC=C(OCC2=C(Br)C=CC=C2)C(/C=C3C(NC(S\3)=S)=O)=C1 | ||
| 分子式 | C17H11Br2NO2S2 | 分子量 | 485.2 |
| 溶解度 | ≤16mg/ml in DMSO;16mg/ml in dimethyl formamide | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.061 mL | 10.305 mL | 20.6101 mL |
| 5 mM | 412.2 μL | 2.061 mL | 4.122 mL |
| 10 mM | 206.1 μL | 1.0305 mL | 2.061 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet