Isodeoxycholic Acid
(Synonyms: isoDCA) 目录号 : GC47464
Isodeoxycholic Acid是一种次级胆汁酸。
Cas No.:566-17-6
Sample solution is provided at 25 µL, 10mM.
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Isodeoxycholic Acid is a secondary bile acid [1]. Isodeoxycholic Acid regulates bile acid synthesis, enterohepatic circulation, and energy metabolism, influencing the composition of the intestinal microbiome and inflammatory responses [2]. Isodeoxycholic Acid is primarily used to treat cholestasis, hepatobiliary diseases, and metabolic syndrome [3-4].
In CD4⁺T cells, Isodeoxycholic Acid (100μM; 3d) acts on dendritic cells, weakening their immunostimulatory properties and thereby enhancing the induction of Foxp3 [5].
References:
[1]. Marschall H U, Broomé U, Einarsson C, et al. Isoursodeoxycholic acid: metabolism and therapeutic effects in primary biliary cirrhosis 1[J]. Journal of Lipid Research, 2001, 42(5): 735-742.
[2]. Devlin A S, Fischbach M A. A biosynthetic pathway for a prominent class of microbiota-derived bile acids[J]. Nature chemical biology, 2015, 11(9): 685-690.
[3]. Hofmann A F, Hagey L R. Bile acids: chemistry, pathochemistry, biology, pathobiology, and therapeutics[J]. Cellular and Molecular Life Sciences, 2008, 65(16): 2461-2483.
[4]. Pabst O, Hornef M W, Schaap F G, et al. Gut–liver axis: barriers and functional circuits[J]. Nature Reviews Gastroenterology & Hepatology, 2023, 20(7): 447-461.
[5]. Campbell C, McKenney P T, Konstantinovsky D, et al. Bacterial metabolism of bile acids promotes peripheral Treg cell generation[J]. Nature, 2020, 581(7809): 475.
Isodeoxycholic Acid是一种次级胆汁酸 [1]。Isodeoxycholic Acid调节胆汁酸合成、肠肝循环和能量代谢，影响肠道菌群的组成和炎症反应 [2]。Isodeoxycholic Acid主要用于治疗胆汁淤积、肝胆疾病和代谢综合征 [3-4]。
在CD4⁺T细胞中，Isodeoxycholic Acid（100μM；3d）作用于树突状细胞，削弱其免疫刺激特性，从而增强Foxp3的诱导 [5]。
| Cell experiment [1]: | |
Cell lines | CD4⁺T cells |
Preparation Method | Naïve CD4⁺T cells (CD44⁻CD62L⁺GFP⁻) were isolated from the spleen and peripheral lymph nodes of Foxp3-GFP mice and co-cultured with dendritic cells (1×105). They were stimulated with Isodeoxycholic Acid (diluted from a 100mM DMSO stock solution) and anti-CD3ε antibody (1μg/mL). T cell proliferation (CellTrace Violet labeling), Foxp3 induction (GFP reporter gene), and cytokine production (PMA/Ionomycin heavy stimulation, Brefeldin A/Monensin secretion blockade) were assessed under different polarization conditions (Treg induction with TGF-β/IL-2 and Th17 induction with TGF-β/IL-6). Flow cytometry was used to quantify cell number using internal control beads to investigate the regulatory effects of Isodeoxycholic Acid on CD4⁺T cell differentiation and function. |
Reaction Conditions | 100μM; 3d |
Applications | Isodeoxycholic Acid acts on dendritic cells, weakening their immunostimulatory properties and thereby enhancing the induction of Foxp3. |
References: | |
| Cas No. | 566-17-6 | SDF | |
| 别名 | isoDCA | ||
| 化学名 | (5β,7α,12α)-7,12-dihydroxy-cholan-24-oic acid | ||
| Canonical SMILES | C[C@@]12[C@@](C[C@@H](O)[C@]3([H])[C@]2([H])C[C@H](O)[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CCC(O)=O)([H])CCCC1 | ||
| 分子式 | C24H40O4 | 分子量 | 392.6 |
| 溶解度 | DMF: 30mg/mL,DMF:PBS (pH 7.2) (1:1): 0.5mg/mL,DMSO: 20mg/mL,Ethanol: 20mg/mL | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.5471 mL | 12.7356 mL | 25.4712 mL |
| 5 mM | 509.4 μL | 2.5471 mL | 5.0942 mL |
| 10 mM | 254.7 μL | 1.2736 mL | 2.5471 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet