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proBDNF Human

目录号 : GP20089

前体脑源性神经营养因子人类重组

proBDNF Human Chemical Structure

规格 价格 库存 购买数量
2μg
¥840.00
5-10工作日
10μg
¥2,030.00
5-10工作日
1mg
¥72,800.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

产品文档

Quality Control & SDS

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产品描述

proBDNF Human Recombinant produced in E.Coli is a single,non-glycosylated, non-covalently linked homodimer with each polypeptide chain containing 229 amino acids and having a molecular mass of 52kDa. The proBDNF is purified by proprietary chromatographic techniques.

Product Data

Purity Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. Source Escherichia Coli.
Phycical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Shipping Condition Shipped at Room temp.
Synonyms proBDNF; Precursor Form Brain-derived Neurotrophic Factor.
Amino Acid Sequence The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Met-Lys.
Solubility It is recommended to reconstitute the lyophilized proBDNF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized proBDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution proBDNF should be stored at 4°C between 2-7 days and for future use below -18°C .Please prevent freeze-thaw cycles.
Formulation proBDNF was lyophilized from a concentrated (0.5mg/ml) solution in 20mM PB, pH 8.0 and 500mM NaCl.

Introduction

proBDNF (the precursor form of Brain-derived neurotrophic factor) interacts preferentially with p75NTR (the pan-neurotrophin receptor p75) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis, while the mature BDNF selectively binds with great affinity to the TrkB kinase receptor and promotes the survival, growth and differentiation of neurons. Since proneurotrophins and mature neurotrophins bring forth opposite biological effects, proBDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. proBDNF has an important role in negative regulation of neurotrophic actions in the brain.

Stability

Lyophilized proBDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution proBDNF should be stored at 4°C between 2-7 days and for future use below -18°C .Please prevent freeze-thaw cycles.