Polyinosinic-polycytidylic acid sodium
(Synonyms: 双链聚肌胞; Poly(I:C)) 目录号 : GC60297
Polyinosinic-polycytidylic acid sodium(Poly I:C) is a synthetic dsRNA that can imitate a viral infection and elicit host immune responses by triggering specific pattern-recognition receptors (PRRs).
Cas No.:42424-50-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cell experiment [1]: | |
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Cell lines |
Hela cells |
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Preparation Method |
Tumor cells were cultured with poly I:C (100 µg/ml) alone, CHX (1.5 µg/ml) alone or poly I:C plus CHX for 72 h. |
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Reaction Conditions |
100 µg/ml;72 h |
|
Applications |
Poly I:C treatment caused tumor cells more sensitive to CHX-induced cell death. |
| Animal experiment [2]: | |
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Animal models |
Four-d-old C57BL/6J pups |
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Preparation Method |
Mice were stressed by asphyxia and hypothermia twice daily. Mice were either fed by formula only (FO), formula containing LPS or poly I:C . After 72 h, mice were euthanized. |
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Dosage form |
10 µg/g;p.o. |
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Applications |
Poly I:C exposure induces severe intestinal injury and inflammation. |
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References: [1]. Jiang Q, Wei H, et,al.Poly I:C enhances cycloheximide-induced apoptosis of tumor cells through TLR3 pathway. BMC Cancer. 2008 Jan 17;8:12. doi: 10.1186/1471-2407-8-12. PMID: 18199340; PMCID: PMC2242792. |
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Polyinosinic-polycytidylic acid sodium(Poly I:C) is a synthetic dsRNA that can imitate a viral infection and elicit host immune responses by triggering specific pattern-recognition receptors (PRRs) such as toll-like receptor 3(TLR3) and retinoic acid inducible gene I(RIG-I)-like receptors, including RIG-I and melanoma differentiation-associated gene 5. Poly I:C can be used as a vaccine adjuvant for cancer immunotherapy[1-3].
poly(I:C)(20 µg in 0.2 mL of cell suspension)-electroporated AML cells potently activate DC and NK cell functions and stimulate NK-DC cross-talk in terms of tumor cell killing[4]. Activation of the TLR3 signaling pathway in cancer cells through poly I:C(100 µg/ml;72 h) makes Hela cells (human cervical cancer) dose-dependent sensitive to apoptosis induced by the protein synthesis inhibitor cycloheximide (CHX) [5].
Poly I:C(10 µg/g;p.o.) exposure induces severe intestinal injury and inflammation[6]. Pre-treatment with the viral TLR3 agonist poly(I:C) (20/200 µg) modulated innate immune responses and strengthened the resistance of neutropenic mice against E. coli K1 meningoencephalitis[7].The intratumoral synergistic combination of poly(I:C)(25 µg;i.p; six times from day9-day21)+R848 significantly prevented tumor growth and metastasis in lung cancer and fibrosarcoma immunocompetent murine models[8].
Polyinosinic-polycytidylic acid sodium (Poly I:C)是一种合成的dsRNA,可以模拟病毒感染,并通过触发特定的模式识别受体(PRRs),如toll样受体3(TLR3)和维甲酸诱导基因I(RIG-I)样受体,包括RIG-I和黑色素瘤分化相关基因5,引发宿主免疫反应。Poly I:C可作为癌症免疫治疗的疫苗佐剂[1-3]。
Poly I:C (20µg)电穿孔AML细胞能有效激活DC和NK细胞功能,并在杀伤肿瘤细胞方面刺激NK-DC串扰[4]。poly I:C(100 µg/ml;72 h)激活癌细胞中TLR3信号通路,使Hela细胞(人宫颈癌)对蛋白质合成抑制剂环己亚胺(cycloheximide, CHX)诱导的凋亡呈剂量依赖性敏感[5]。
Poly I:C(10µg/g;p.o.)可引起严重的肠道损伤和炎症[6]。用病毒性TLR3激动剂poly(I:C)(20/200µg)预处理可调节先天免疫反应,增强中性粒细胞减少小鼠对大肠杆菌K1脑膜脑炎的抵抗力[7]。肿瘤内poly(I:C) (25 µg;i.p; six times from day9-day21)+R848的协同组合可显著阻止肺癌和纤维肉瘤免疫活性小鼠模型的肿瘤生长和转移[8]。
References:
[1]. Zhao X, Ai M, et,al. Poly I:C-induced tumor cell apoptosis mediated by pattern-recognition receptors. Cancer Biother Radiopharm. 2012 Nov;27(9):530-4. doi: 10.1089/cbr.2012.1226. Epub 2012 Oct 12. PMID: 23062195.
[2]. Zhao X, Ai M, et,al.Poly I:C-induced tumor cell apoptosis mediated by pattern-recognition receptors. Cancer Biother Radiopharm. 2012 Nov;27(9):530-4. doi: 10.1089/cbr.2012.1226. Epub 2012 Oct 12. PMID: 23062195.
[3]. Kato H, Takeuchi O, et,al.Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses. Nature. 2006 May 4;441(7089):101-5. doi: 10.1038/nature04734. Epub 2006 Apr 9. PMID: 16625202.
[4]. Lion E, Anguille S, et,al.Poly(I:C) enhances the susceptibility of leukemic cells to NK cell cytotoxicity and phagocytosis by DC. PLoS One. 2011;6(6):e20952. doi: 10.1371/journal.pone.0020952. Epub 2011 Jun 17. PMID: 21698118; PMCID: PMC3117863.
[5]. Jiang Q, Wei H, et,al.Poly I:C enhances cycloheximide-induced apoptosis of tumor cells through TLR3 pathway. BMC Cancer. 2008 Jan 17;8:12. doi: 10.1186/1471-2407-8-12. PMID: 18199340; PMCID: PMC2242792.
[6]. Ginzel M, Yu Y, et,al.The viral dsRNA analogue poly (I:C) induces necrotizing enterocolitis in neonatal mice. Pediatr Res. 2016 Apr;79(4):596-602. doi: 10.1038/pr.2015.261. Epub 2015 Dec 17. PMID: 26679153.
[7]. Ribes S, Arcilla C, et,al.Pre-treatment with the viral Toll-like receptor 3 agonist poly(I:C) modulates innate immunity and protects neutropenic mice infected intracerebrally with Escherichia coli. J Neuroinflammation. 2020 Jan 17;17(1):24. doi: 10.1186/s12974-020-1700-4. PMID: 31952519; PMCID: PMC6969464.
[8]. Anfray C, Mainini F, et,al.Intratumoral combination therapy with poly(I:C) and resiquimod synergistically triggers tumor-associated macrophages for effective systemic antitumoral immunity. J Immunother Cancer. 2021 Sep;9(9):e002408. doi: 10.1136/jitc-2021-002408. PMID: 34531246; PMCID: PMC8449972.
| Cas No. | 42424-50-0 | SDF | |
| 别名 | 双链聚肌胞; Poly(I:C) | ||
| 化学名 | 5'-Inosinic acid, homopolymer, complex with 5'-cytidylic acid homopolymer, sodium salt (1:1:x) | ||
| Canonical SMILES | [Polyinosinic-polycytidylic acid (sodium)] | ||
| 分子式 | (C10H13N4O8P)x.(C9H14N3O8P)x.xNa | 分子量 | 671.40 |
| 溶解度 | Water : 10 mg/mL (Need ultrasonic and warming) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.4894 mL | 7.4471 mL | 14.8943 mL |
| 5 mM | 0.2979 mL | 1.4894 mL | 2.9789 mL |
| 10 mM | 0.1489 mL | 0.7447 mL | 1.4894 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Anti-tumour effects of exosomes in combination with cyclophosphamide and polyinosinic-polycytidylic acid
J Int Med Res 2008 Nov-Dec;36(6):1342-53.PMID:19094445DOI:10.1177/147323000803600623.
We examined the anti-tumour activity of exosomes derived from dendritic cells (DCs) in combination with cyclophosphamide (CTX) and Polyinosinic-polycytidylic acid sodium salt (poly I:C). DCs were pulsed with L1210 lymphocytic leukaemia cell antigen and lipopolysaccharide. The exosomes that the DCs secreted were purified. In vitro, the anti-tumour activity of exosomes was assessed by measuring their ability to induce spleen cell proliferation and the extent to which they induced spleen cells to kill L1210 cells. Poly I:C was able to induce DC maturation. DC-derived exosomes stimulated spleen cell proliferation and enhanced the cytotoxic effects of spleen cells in vitro. DC-derived exosomes, in combination with CTX and poly I:C, suppressed L1210 tumour growth in vivo and gave the greatest prolongation of survival time in tumour-bearing DBA2 mice. These findings suggest that this combination of a tumour vaccine, a conventional anti-cancer agent and a promoter of DC maturation might be a useful anti-cancer therapy.
Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles
Beilstein J Nanotechnol 2022 Jan 18;13:82-95.PMID:35116215DOI:10.3762/bjnano.13.6.
Polyethylenimine (PEI), which is frequently used for polyplex formation and effective gene transfection, is rarely recognized as a luminescent polymer. Therefore, it is usually tagged with an organic fluorophore to be optically tracked. Recently, we developed branched PEI (bPEI) superparamagnetic iron oxide nanoparticles (SPION@bPEI) with blue luminescence 1200 times stronger than that of bPEI without a traditional fluorophore, due to partial PEI oxidation during the synthesis. Here, we demonstrate in vitro dye-free optical imaging and successful gene transfection with luminescent SPION@bPEI, which was further modified for receptor-mediated delivery of the cargo selectively to cancer cell lines overexpressing the epidermal growth factor receptor (EGFR). Pro-apoptotic Polyinosinic-polycytidylic acid sodium (PIC) was delivered to HeLa cells with SPION@bPEI and caused a dramatic reduction in the cell viability at otherwise non-toxic nanoparticle concentrations, proving that bPEI coating is still an effective component for the delivery of an anionic cargo. Besides, a strong intracellular optical signal supports the optically traceable nature of these nanoparticles. SPION@bPEI nanoparticles were further conjugated with Erbitux (Erb), which is an anti-EGFR antibody for targeting EGFR-overexpressing cancer cell lines. SPION@bPEI-Erb was used for the delivery of a GFP plasmid wherein the transfection was confirmed by the luminescence of the expressed gene within the transfected cells. Poor GFP expression in MCF7, a slightly better expression in HeLa, and a significant enhancement in the transfection of HCT116 cells proved a selective uptake and hence the targeting ability of Erb-tagged nanoparticles. Altogether, this study proves luminescent, cationic, and small SPION@bPEI nanoparticles as strong candidates for imaging and gene therapy.