Phenazine methylsulfate
(Synonyms: 吩嗪硫酸甲酯; 5-Methylphenazinium methylsulfate) 目录号 : GC61179
A free radical generator
Cas No.:299-11-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Phenazine is a free radical generator.1 It has been used as an electron transfer reactant in cell viability assays.2 Phenazine (10 ?M) induces ssDNA break formation in the presence of the reducing agent NADPH in a cell-free plasmid cleavage assay when used at a concentration of 10 ?M. It induces oxidative DNA damage in an alkaline comet assay and apoptosis in A375 melanoma cells when used at a concentration of 10 ?M. Phenazine (20 nM) oxidizes cysteine-containing proteins in HepG2 cells.1
1.Lin, C.-Y., Hu, C.-T., Cheng, C.-C., et al.Oxidation of heat shock protein 60 and protein disulfide isomerase activates ERK and migration of human hepatocellular carcinoma HepG2Oncotarget7(10)11067-11082(2016) 2.Hua, A.B., Justiniano, R., Perer, J., et al.Repurposing the electron transfer reactant phenazine methosulfate (PMS) for the apoptotic elimination of malignant melanoma cells through induction of lethal oxidative and mitochondriotoxic stressCancers (Basel)11(5)E590(2019)
| Cas No. | 299-11-6 | SDF | |
| 别名 | 吩嗪硫酸甲酯; 5-Methylphenazinium methylsulfate | ||
| Canonical SMILES | C[N+]1=C2C=CC=CC2=NC3=C1C=CC=C3.O=S(OC)([O-])=O | ||
| 分子式 | C14H14N2O4S | 分子量 | 306.34 |
| 溶解度 | DMSO: 62.5 mg/mL (204.02 mM) | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.2643 mL | 16.3217 mL | 32.6435 mL |
| 5 mM | 0.6529 mL | 3.2643 mL | 6.5287 mL |
| 10 mM | 0.3264 mL | 1.6322 mL | 3.2643 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Peroxiredoxin-linked detoxification of hydroperoxides in Toxoplasma gondii
J Biol Chem 2005 Jan 7;280(1):564-70.PMID:15507457DOI:10.1074/jbc.M406367200.
The apicomplexan parasite Toxoplasma gondii is highly susceptible to oxidative stress caused by tert-butyl-hydroperoxide, juglone, and Phenazine methylsulfate with IC(50) in the nanomolar range. Using dichlorofluorescein diacetate, a detector of endogenous oxidative stress, it was shown that juglone and Phenazine methylsulfate are potentially toxic to the parasites without affecting the host cells. These results demonstrate that T. gondii is vulnerable to oxidative challenge that results from disruption of its redox balance and so this could be an effective approach to therapeutic intervention. This study has characterized redox active and antioxidant peroxidases belonging to the class of 1-Cys and 2-Cys peroxiredoxins that play crucial roles in maintaining redox balance. The tachyzoite stages of T. gondii express thioredoxin (TgTrx), 1-Cys peroxiredoxin (TgTrx-Px2), and a 2-Cys peroxiredoxin (TgTrx-Px1) and immunofluorescent studies revealed that all three proteins are located in the cytosol of the parasite confirming previous studies on TgTrx-Px1 (Kwok, L.Y., Schluter, D., Clayton, C., and Soldati, D. (2004) Mol. Microbiol. 51, 47-61). TgTrx-Px1 showed K(m) values for H(2)O(2) and tert-butyl hydroperoxide in the nanomolar range, emphasizing the great affinity of the protein for theses substrates. Moreover, the catalytic efficiency of TgTrx-Px1 for these substrates at 10(6)-10(7) M(-1) s(-1) is unusually high, which qualifies the enzyme as an extremely potent antioxidant. Kinetic analyses revealed that TgTrx-Px1 is inhibited by tert-butyl hydroperoxide, and apparent inhibition constants were determined to be between 33 and 35.6 microm depending on the concentration of the non-inhibitory substrate thioredoxin. TgTrx-Px2 protected glutamine synthetase from inactivation by Fe(3+)/DTT, showing that it is an active peroxiredoxin.
[Comparative study of the effect of phenazine methosulfate and vitamin K3 on human erythrocytes by hemolysis curves]
Biofizika 1991 Nov-Dec;36(6):1048-50.PMID:1809383doi
Hemolysis curves were used for comparative study of Phenazine methylsulfate (PMS) and vitamin K3 action on human erythrocytes. Some differences in PMS and vitamin K3 action were revealed while the concentration of studied compounds and incubation time with them were changed. It is considered that the observed differences in PMS and vitamin K3 action are caused by different degree of oxidation of intracellular hemoglobin.