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Phalloidin

(Synonyms: 鬼笔环肽) 目录号 : GC15692

Phalloidin binds F-actin and stabilizes actin fibers.

Phalloidin Chemical Structure

Cas No.:17466-45-4

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1mg
¥2,903.00
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Sample solution is provided at 25 µL, 10mM.

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实验参考方法

Cell experiment:

Prepare a 1:200 dilution of the stock solution (300 units/mL) of fluorescein Phalloidin or rhodamine Phalloidin using PBS. Aspirate the cell medium from the cells grown on glass coverslips and rinse the cells three times with PBS. Fix the cells for 10 min in the 3.7% formaldehyde solution. Rinse the fixed cells three times for 5 min each in PBS. Permeabilize the cells for 5 min in the 0.2% Triton X-100 solution. Rinse the fixed cells three times with PBS. Label the cells with the fluorescein or rhodamine Phalloidin for 5 to 10 min at room temperature. Rinse the cells three times in PBS for 5 min each time. Mount the cell and view the cells using either the fluorescein or rhodamine filter set, depending on the probe[1].

References:

[1]. Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging. Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot4947.

产品描述

Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome(λex=495 nm, λem=520 nm).

Phalloidin staining will not work with methanol-fixed cells, probably because of the disruption of actin filament integrity, but it will work with cells fixed in 0.2% glutaraldehyde in PBS. For fluorescein isothiocyanate (FITC)-Phalloidin (or rhodamine isothiocyanate [RITC]-Phalloidin), the excitation wavelength maximum will be near 495 nm (or 550 nm) and the emission wavelength maximum will be ~520 nm based on H2O at pH 8.0 as the solvent (575 nm with methanol as the solvent). The emission spectrum of fluorescein can depend on the environmental pH and/or ionic strength[1].

References:
[1]. Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging. Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot4947.

Chemical Properties

Cas No. 17466-45-4 SDF
别名 鬼笔环肽
化学名 phalloidin
Canonical SMILES O[C@@H]1C[C@H](C(N[C@@H](C)C(N[C@@H](C(N[C@@H](C(N[C@@H]2C)=O)C[C@](CO)(C)O)=O)CC3=C(NC4=CC=CC=C34)SC[C@H]5NC([C@H]([C@H](C)O)NC2=O)=O)=O)=O)N(C5=O)C1
分子式 C35H48N8O11S 分子量 788.87
溶解度 10mg/mL in ethanol, or in DMSO, or in DMF , 0.5 mg/ml in warm water 储存条件 Store at -20°C,protect from light
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1 mM 1.2676 mL 6.3382 mL 12.6764 mL
5 mM 0.2535 mL 1.2676 mL 2.5353 mL
10 mM 0.1268 mL 0.6338 mL 1.2676 mL
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