MutS
目录号 : GP23995Thermus Aquaticus DNA 错配修复蛋白 MutS 重组体
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 95.0% as determined by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile filtered colorless solution. | Shipping Condition | Shipped with Ice Packs. |
| Synonyms | MutS; Thermus Aquaticus DNA Mismatch Repair Protein; DNA mismatch repair protein MutS. | ||
| Amino Acid Sequence | MKIEHMEGML KGEGPGPLPP LLQQYVELRD QYPDYLLLFQ VGDFYECFGE DAERLARALG LVLTHKTSKD FTTPMAGIPL RAFEAYAERL LKMGFRLAVA DQVEPAEEAE GLVRREVTQL LTPGTLLQES LLPREANYLA AIATGDGWGL AFLDVSTGEF KGTVLKSKSA LYDELFRHRP AEVLLAPELL ENGAFLDEFR KRFPVMLSEA PFEPEGEGPL ALRRARGALL AYAQRTQGGA LSLQPFRFYD PGAFMRLPEA TLRALEVFEP LRGQDTLFSV LDETRTAPGR RLLQSWLRHP LLDRGPLEAR LDRVEGFVRE GALREGVRRL LYRLADLERL ATRLELGRAS PKDLGALRRS LQILPELRAL LGEEVGLPDL SPLKEELEAA LVEDPPLKVS EGGLIREGYD PDLDALRAAH REGVAYFLEL EERERERTGI PTLKVGYNAV FGYYLEVTRP YYERVPKEYR PVQTLKDRQR YTLPEMKEKE REVYRLEALI RRREEEVFLE VRERAKRQAE ALREAARILA ELDVYAALAE VAVRYGYVRP RFGDRLQIRA GRHPVVERRT EFVPNDLEMA HELVLITGPN MAGKSTFLRQ TALIALLAQV GSFVPAEEAH LPLFDGIYTR IGASDDLAGG KSTFMVEMEE VALILKEATE NSLVLLDEVG RGTSSLDGVA IATAVAEALH ERRAYTLFAT HYFELTALGL PRLKNLHVAA REEAGGLVFY HQVLPGPASK SYGVEVAAMA GLPKEVVARA RALLQAMAAR REGALDAVLE RLLALDPDRL TPLEALRLLQ ELKALALGAP LDTMKGKLAA ALEHHHHHH. | ||
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. | ||
| Formulation | The MutS protein is supplied in 20mM Tris-HCl, pH-8, 250mM NaCl, 0.1mM EDTA, 1mM DTT and 50% Glycerol. | ||
The MutS DNA Mismatch Protein Identifies heteroduplex DNAs comprising mis-paired or unpaired bases. The MutS DNA Mismatch Protein binds in vitro to heteroduplex DNAs comprising mis-paired or unpaired bases over a varied temperature range from 4-70 °C and obtains a thermostable ATPase activity. The MutS DNA Mismatch Protein is active at temperature between 0 to 75°C. Since The MutS DNA Mismatch Protein competently binds to 1-4 bases deletion (or insertion) and mismatch base pairs of GT, CT and AG, it is suitable for sensing these mutations. Mutations can be distinguished in polyacrylamide gels or on a solid phase such as Ni agarose or beads or magnetic Ni-NTA particles.
Recommended Applications:1. Remove mismatch DNA (error correction) from gene synthesis reaction2. Mutation detection and removal3. Rapid isothermal SNP detection
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.