MoTP
(Synonyms: 4(4Morpholinylbutylthio)phenol) 目录号 : GC44245A tool for ablating zebrafish larval melanocytes
Cas No.:57055-82-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
MoTP, known more formally as 4-(4-morpholinobutylthio)phenol, is used to ablate larval melanocytes in zebrafish in order to study melanocyte regeneration. MoTP is converted to a cytotoxin in cells with high tyrosinase activity, which in the zebrafish embryo are limited to melanoblasts and newly formed melanocytes. The wash-out of MoTP allows regeneration through the proliferation of melanocyte stem cells.
| Cas No. | 57055-82-0 | SDF | |
| 别名 | 4(4Morpholinylbutylthio)phenol | ||
| Canonical SMILES | OC1=CC=C(SCCCCN2CCOCC2)C=C1 | ||
| 分子式 | C14H21NO2S | 分子量 | 267.4 |
| 溶解度 | DMF: 33 mg/ml,DMSO: 25 mg/ml,Ethanol: 0.3 mg/ml,PBS (pH 7.2): 5 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.7397 mL | 18.6986 mL | 37.3972 mL |
| 5 mM | 0.7479 mL | 3.7397 mL | 7.4794 mL |
| 10 mM | 0.374 mL | 1.8699 mL | 3.7397 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
MoTP Subunit is Critical for Ion Selectivity and Evolution of a K+-Coupled Flagellar Motor
Biomolecules 2020 Apr 29;10(5):691.PMID:32365619DOI:10.3390/biom10050691.
The bacterial flagellar motor is a sophisticated nanomachine embedded in the cell envelope. The flagellar motor is driven by an electrochemical gradient of cations such as H+, Na+, and K+ through ion channels in stator complexes embedded in the cell membrane. The flagellum is believed to rotate as a result of electrostatic interaction forces between the stator and the rotor. In bacteria of the genus Bacillus and related species, the single transmembrane segment of MotB-type subunit protein (MotB and MotS) is critical for the selection of the H+ and Na+ coupling ions. Here, we constructed and characterized several hybrid stators combined with single Na+-coupled and dual Na+- and K+-coupled stator subunits, and we report that the MoTP subunit is critical for the selection of K+. This result suggested that the K+ selectivity of the MoTP/MotS complexes evolved from the single Na+-coupled stator MoTP/MotS complexes. This finding will promote the understanding of the evolution of flagellar motors and the molecular mechanisms of coupling ion selectivity.
Multiple Traffic Target Tracking with Spatial-Temporal Affinity Network
Comput Intell Neurosci 2022 May 23;2022:9693767.PMID:35655505DOI:10.1155/2022/9693767.
Traffic target tracking is a core task in intelligent transportation system because it is useful for scene understanding and vehicle autonomous driving. Most state-of-the-art (SOTA) multiple object tracking (MOT) methods adopt a two-step procedure: object detection followed by data association. The object detection has made great progress with the development of deep learning. However, the data association still heavily depends on hand crafted constraints, such as appearance, shape, and motion, which need to be elaborately trained for a special object. In this study, a spatial-temporal encoder-decoder affinity network is proposed for multiple traffic targets tracking, aiming to utilize the power of deep learning to learn a robust spatial-temporal affinity feature of the detections and tracklets for data association. The proposed spatial-temporal affinity network contains a two-stage transformer encoder module to encode the features of the detections and the tracked targets at the image level and the tracklet level, aiming to capture the spatial correlation and temporal history information. Then, a spatial transformer decoder module is designed to compute the association affinity, where the results from the two-stage transformer encoder module are fed back to fully capture and encode the spatial and temporal information from the detections and the tracklets of the tracked targets. Thus, efficient affinity computation can be applied to perform data association in online tracking. To validate the effectiveness of the proposed method, three popular multiple traffic target tracking datasets, KITTI, UA-DETRAC, and VisDrone, are used for evaluation. On the KITTI dataset, the proposed method is compared with 15 SOTA methods and achieves 86.9% multiple object tracking accuracy (MOTA) and 85.71% multiple object tracking precision (MoTP). On the UA-DETRAC dataset, 12 SOTA methods are used to compare with the proposed method, and the proposed method achieves 20.82% MOTA and 35.65% MoTP, respectively. On the VisDrone dataset, the proposed method is compared with 10 SOTA trackers and achieves 40.5% MOTA and 74.1% MoTP, respectively. All those experimental results show that the proposed method is competitive to the state-of-the-art methods by obtaining superior tracking performance.
Metabolism and disposition of (RS)-2-methoxy-3-(octadecylcarbamoyloxy)propyl 2-(3-thiazolio)ethyl phosphate (MoTP) in rats and dogs
Xenobiotica 1988 Jan;18(1):49-59.PMID:3354232DOI:10.3109/00498258809055136.
1. Metabolites (RS)-4-[(3-hydroxy-2-methoxy)propoxycarbonylamino]butanoic acid (I) and (RS)-2-[(3-hydroxy-2-methoxy)propoxycarbonylamino]acetic acid(II) were isolated from urine after i.v. administration of (RS)-2-methoxy-3-(octadecyl-[14C]carbamoyloxy)propyl 2-(3-thiazolio)ethyl phosphate (14C-MOTP) to rats and characterized by t.l.c., g.l.c.-mass spectrometry and p.m.r. spectrometry. 2. After i.v. administration of 14C-MOTP, the plasma concentration of the drug declined biphasically with half-lives of 0.22 and 3.94 h in rats, and 0.81 and 8.00 h in dogs. In rats and dogs, unchanged MoTP was the main 14C component in the plasma, together with a small amount of I and II. 14C-MOTP was highly bound to plasma protein of both animals. 3. Five min after i.v. administration of 14C-MOTP to rats, 14C was widely distributed in tissues, with the highest conc. in the lung and the lowest in the eye. The distribution of 14C was relatively slow in some tissues. In most tissues, 14C decreased to low levels at 96 h, except in the Harder's gland. 4. Elimination of 14C-MOTP was almost complete within 120 h in rats and 144 h in dogs. In both species, the administered 14C was excreted largely in the urine as I and II, with the remainder appearing in the faeces and the expired air. Biliary excretion and reabsorption of 14C were detected in rats. 5. During repeated i.v. administration of 14C-MOTP to rats for 7 days, the conc. of 14C in plasma and most tissues attained steady state within 5 days, except in Harder's gland, where the level rose gradually until the seventh day of dosing. Within 6 days after the last dosing, 96% of the injected dose was eliminated from the body.
Mutational analysis of charged residues in the cytoplasmic loops of MotA and MoTP in the Bacillus subtilis flagellar motor
J Biochem 2014 Oct;156(4):211-20.PMID:24771657DOI:10.1093/jb/mvu030.
Bacterial flagellar motors are energized by a proton (H(+)) or sodium ion (Na(+)) motive force. The motor torque is generated by the interactions between a rotor and about a dozen stators at the interface. MotAB-type stators use H(+), whereas MotPS- and PomAB-type stators use Na(+) as the coupling ion. In Escherichia coli, the cytoplasmic loop of MotA contains charged residues that interact with conserved charged residues in a rotor protein FliG. Bacillus subtilis has two distinct stator elements MotAB and MotPS. Both stator elements contribute to torque generation by the flagellar motor. To clarify the roles of conserved charged residues in the cytoplasmic loops of MotA and MoTP in flagellar rotation, we performed site-directed mutagenesis and analysed motility as well as the relative expression levels of mutant Mot proteins. The motility of the majority of these mutants was reduced compared with that of the wild-type, but was observed at a significant level compared with that of a ΔmotAB ΔmotPS mutant. From the expression levels and the decrease in the motility, we propose that MotA-E98, MotA-E102, MotP-R94, MotP-K95 and MotP-E107 may be responsible for flagellar rotation.
An Efficient Two-Factor Authentication Scheme Based on the Merkle Tree
Sensors (Basel) 2020 Oct 9;20(20):E5735.PMID:33050225DOI:10.3390/s20205735.
The Time-based One-Time Password (TOTP) algorithm is commonly used for two-factor authentication. In this algorithm, a shared secret is used to derive a One-Time Password (OTP). However, in TOTP, the client and the server need to agree on a shared secret (i.e., a key). As a consequence, an adversary can construct an OTP through the compromised key if the server is hacked. To solve this problem, Kogan et al. proposed T/Key, an OTP algorithm based on a hash chain. However, the efficiency of OTP generation and verification is low in T/Key. In this article, we propose a novel and efficient Merkle tree-based One-Time Password (MoTP) algorithm to overcome such limitations. Compared to T/Key, this proposal reduces the number of hash operations to generate and verify the OTP, at the cost of small server storage and tolerable client storage. Experimental analysis and security evaluation show that MoTP can resist leakage attacks against the server and bring a tiny delay to two-factor authentication and verification time.