Misoprostol-d5
(Synonyms: SC-29333-d5) 目录号 : GC47682
A neuropeptide with diverse biological activities
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Misoprostol-d5 is intended for use as an internal standard for the quantification of misoprostol by GC- or LC-MS. Misoprostol is an analog of prostaglandin E1 and an agonist of the PGE2 receptor subtypes EP2 and EP3.1,2,3 It binds to EP1, EP2, EP3-III, and EP4 receptors (Kis = 35.675, 10.249, 0.319, 5.499 μM, respectively) and is selective for EP receptors over DP, FP, IP, and TP receptors (Kis = >100 μM for all).1 Misoprostol inhibits electrically induced twitch contraction in isolated guinea pig ileum circular muscle and vas deferens (EC50s = 102.92 and 4.3 nM, respectively), which endogenously express high levels of EP2 and EP3 receptors, respectively.3,4 It inhibits FMLP-induced superoxide anion generation in human neutrophils (EC50 = 0.35 μM).2 Misoprostol inhibits ethanol-induced gastric lesion formation in rats (ED50 = 0.31 μg/kg).5 Formulations containing misoprostol have been used in the prevention of NSAID-induced gastric ulcers.
1.Abramovitz, M., Adam, M., Boie, Y., et al.The utilization of recombinant prostanoid receptors to determine the affinities and selectivities of prostaglandins and related analogsBiochim. Biophys. Acta1483(2)285-293(2000) 2.Talpain, E., Armstrong, R.A., Coleman, R.A., et al.Characterization of the PGE receptor subtype mediating inhibition of superoxide production in human neutrophilsBr. J. Pharmacol.114(7)1459-1465(1995) 3.Savage, M.A., Moummi, C., Karabatsos, P.J., et al.SC-46275: A potent and highly selective agonist at the EP3 receptorProstaglandins Leukot. Essent. Fatty Acids49(6)939-943(1993) 4.Nials, A.T., Coleman, R.A., Hartley, D., et al.AH13205 - a novel selective prostanoid EP2 agonistBr. J. Pharmacol.10224P(1991) 5.Bunce, K.T., Clayton, N.M., Coleman, R.A., et al.GR63799X - a novel prostanoid with selectivity for EP3 receptorsAdv. Prostaglandin Thromboxane Leukot. Res.21(A)379-382(1990)
| Cas No. | N/A | SDF | |
| 别名 | SC-29333-d5 | ||
| Canonical SMILES | CCCC([2H])([2H])C(O)(C([2H])([2H])[2H])C/C=C/[C@H]([C@H]1CCCCCCC(OC)=O)[C@H](O)CC1=O | ||
| 分子式 | C22H33D5O5 | 分子量 | 387.6 |
| 溶解度 | DMF: 100 mg/ml,DMSO: 50 mg/ml,Ethanol: 50 mg/ml,PBS (pH 7.2): 1.6 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.58 mL | 12.8999 mL | 25.7998 mL |
| 5 mM | 0.516 mL | 2.58 mL | 5.16 mL |
| 10 mM | 0.258 mL | 1.29 mL | 2.58 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。