MIP 1b Human
目录号 : GP21179巨噬细胞炎症蛋白-1 Beta 人类重组 (CCL4)
Sample solution is provided at 25 µL, 10mM.
Purity | Greater than 99.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | Source | Escherichia Coli. |
Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
Synonyms | Small inducible cytokine A4; CCL4; Macrophage inflammatory protein 1-beta; MIP-1- beta; MIP-1-beta(1-69); T-cell activation protein 2; ACT-2; PAT 744; H400; SIS-gamma; Lymphocyte activation gene 1 protein; LAG-1; HC21; G-26 T-lymphocyte-secreted protein; chemokine (C-C motif) ligand 4; ACT2; G-26; LAG1; MIP1B; SCYA4; AT744.1; MGC104418; MGC126025; MGC126026. | ||
Amino Acid Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Pro-Met-Gly-Ser. | ||
Solubility | It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
Stability | Lyophilized MIP-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution CCL4 should be stored at 4°C between 2-7 days and for future use below -18°C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
Biological Activity | The Activity is calculated by the ability to chemoattract Human blood monocytes using a concentration of 5-20ng/ml corresponding to a Specific Activity of 50,000-200,000IU/mg. | ||
Formulation | Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. |
Macrophage Inflammatory Proteins belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1a and MIP-1b that are now also named CCL3 and CCL4. Both factors are produced by macrophages after they are stimulated with bacterial endotoxins. MIP-1a and MIP-1b activate human granulocytes (neutrophils, eosinophils and basophils) which can lead to acute neutrophilic inflammation. MIP-1a and MIP-1b induce synthesis and release of other pro-inflammatory cytokines such as interleukin-1 (IL-1), IL-6 and TNF-alpha from fibroblasts and macrophages. CCL3 and CCL4 genes are both located on human chromosome 17.
The Activity is calculated by the ability to chemoattract Human blood monocytes using a concentration of 5-20ng/ml corresponding to a Specific Activity of 50,000-200,000IU/mg.
Lyophilized MIP-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution CCL4 should be stored at 4°C between 2-7 days and for future use below -18°C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.