Methyl cholate
(Synonyms: 胆酸甲酯) 目录号 : GC38960
Methyl Cholate (Cholic acid methyl ester) is methyl ester form of Cholic acid that is a primary bile acid mainly produced by the liver.
Cas No.:1448-36-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Methyl Cholate (Cholic acid methyl ester) is methyl ester form of Cholic acid that is a primary bile acid mainly produced by the liver.
| Cas No. | 1448-36-8 | SDF | |
| 别名 | 胆酸甲酯 | ||
| Canonical SMILES | O[C@H]1[C@@]2([H])[C@@]3([H])[C@@]([C@]([C@H](C)CCC(OC)=O)([H])CC3)([C@@H](O)C[C@]2([H])[C@]4(C)[C@](C[C@H](O)CC4)([H])C1)C | ||
| 分子式 | C25H42O5 | 分子量 | 422.6 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.3663 mL | 11.8315 mL | 23.663 mL |
| 5 mM | 0.4733 mL | 2.3663 mL | 4.7326 mL |
| 10 mM | 0.2366 mL | 1.1832 mL | 2.3663 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Biotransformation of Methyl cholate by Aspergillus niger
Steroids 2009 Apr-May;74(4-5):483-6.PMID:19428436DOI:10.1016/j.steroids.2009.01.002.
Biotransformation of Methyl cholate using Aspergillus niger was investigated. This led to the isolation of two derivatives: methyl 3alpha,7alpha,12alpha,15beta-tetrahydroxy-5beta-cholan-24-oate identified as a new compound, and a known compound methyl 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oate. The structure elucidation of the new compound was achieved using 1D and 2D NMR, MS and X-ray diffraction.
Mixed association of cholesterol with Methyl cholate and methyl lithocholate in chloroform solutions
Biophys Chem 1982 Dec;16(4):317-28.PMID:7159680DOI:10.1016/0301-4622(82)87036-1.
The concentration-dependent mixed association behavior of cholesterol with Methyl cholate (MeC) and methyl lithocholate (MeLC) in chloroform at 37 degrees C has been studied by vapor pressure osmometry (VPO). This study is part of a larger project to investigate the effect of number and position of hydroxyl-bearing steroids. Using theories developed by Adams and by Steiner, the model and appropriate parameters for the nonideal mixed associations were elucidated. For the MeLC/cholesterol system, no mixed association was observed. For the MeC/cholesterol system, both methods of analysis indicate that a nonideal AB complex formation occurs. The best parameters to explain the experimental data are kAB = 0.04 1/g; BAB (the nonideal term) = 1.5 X 10(-5) 1 mol g-2.
Exchange of methyl hydrogens in ethanol during incorporation in bile acids in vivo
Biochim Biophys Acta 1979 Nov 21;575(2):193-203.PMID:508782DOI:10.1016/0005-2760(79)90021-3.
[2,2,2-2H]Ethanol was administered continuously to bile fistula rats for 72 h, with or without (--)-hydroxycitrate. The deuterium labelling of biliary bile acids was determined by GC-MS and 13C NMR. Difference spectra between 2H,1H- and 1H-decoupled 13C NMR spectra showed the presence of partly deuterated methyl and methylene groups in Methyl cholate, indicating exchange of deuterium in [2,2,2-2H]ethanol for protium prior to or during incorporation of acetate into the bile acid. The extent of exchange was 20--30% as calculated from the isotopic composition of a fragment ion containing one methyl and one methylene group derived from C-2 of acetate. The exchange was unaffected by (--)-hydroxycitrate, indicating that it was not due to reversible incorporation of deuterated acetate into citrate. About 60% of the acetyl-CoA serving as precursor of cholic and chenodeoxycholic acids were derived from ethanol. This value was not changed by administration of (--)-hydroxycitrate. The half-life time of cholesterol molecules acting as precursors of both bile acids was about 50 h in the presence of (--)-hydroxycitrate, which is about the same as previously found in the absence of the inhibitor.
A Network Pharmacology to Explore the Mechanism of Calculus Bovis in the Treatment of Ischemic Stroke
Biomed Res Int 2021 Mar 10;2021:6611018.PMID:33778069DOI:10.1155/2021/6611018.
Background: Calculus Bovis is a valuable Chinese medicine, which is widely used in the clinical treatment of ischemic stroke. The present study is aimed at investigating its target and the mechanism involved in ischemic stroke treatment by network pharmacology. Methods: Effective compounds of Calculus Bovis were collected using methods of network pharmacology and using the Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine (BATMAN-TCM) and the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). Potential compound targets were searched in the TCMSP and SwissTargetPrediction databases. Ischemic stroke-related disease targets were searched in the Drugbank, DisGeNet, OMIM, and TTD databases. These two types of targets were uploaded to the STRING database, and a network of their interaction (PPI) was built with its characteristics calculated, aiming to reveal a number of key targets. Hub genes were selected using a plug-in of the Cytoscape software, and Gene Ontology (GO) biological processes and pathway enrichment analyses of Kyoto Encyclopedia of Genes and Genomes (KEGG) were conducted using the clusterProfiler package of R language. Results: Among 12 compounds, deoxycorticosterone, Methyl cholate, and biliverdin were potentially effective components. A total of 344 Calculus Bovis compound targets and 590 ischemic stroke targets were found with 92 overlapping targets, including hub genes such as TP53, AKT, PIK2CA, MAPK3, MMP9, and MMP2. Biological functions of Calculus Bovis are associated with protein hydrolyzation, phosphorylation of serine/threonine residues of protein substrates, peptide bond hydrolyzation of peptides and proteins, hydrolyzation of intracellular second messengers, antioxidation and reduction, RNA transcription, and other biological processes. Conclusion: Calculus Bovis may play a role in ischemic stroke by activating PI3K-AKT and MAPK signaling pathways, which are involved in regulating inflammatory response, cell apoptosis, and proliferation.
A cholesterol biosynthesis inhibitor from Rhizopus oryzae
Arch Pharm Res 2004 Jun;27(6):624-7.PMID:15283464DOI:10.1007/BF02980161.
A bile acid derivative, Methyl cholate (1), was isolated from EtOAc extract of the fungus Rhizopus oryzae as a cholesterol biosynthesis inhibitor. It showed moderate inhibitory activity on cholesterol biosynthesis in human Chang liver cells. Compound 1 exhibited inhibitory effect on the later step of cholesterol biosynthesis, indicating that its action mode is different from that of statins that act on the HMG-CoA reductase.