Lotusine
(Synonyms: 莲心季铵碱) 目录号 : GC39007
#N/A
Cas No.:6871-67-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cas No. | 6871-67-6 | SDF | |
| 别名 | 莲心季铵碱 | ||
| Canonical SMILES | C[N+]1(C)[C@H](CC2=CC=C(O)C=C2)C3=C(C=C(O)C(OC)=C3)CC1 | ||
| 分子式 | C19H24NO3+ | 分子量 | 314.4 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1807 mL | 15.9033 mL | 31.8066 mL |
| 5 mM | 0.6361 mL | 3.1807 mL | 6.3613 mL |
| 10 mM | 0.3181 mL | 1.5903 mL | 3.1807 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Inhibitory Effect of Lotusine on Solar UV-Induced Matrix Metalloproteinase-1 Expression
Plants (Basel) 2022 Mar 14;11(6):773.PMID:35336655DOI:10.3390/plants11060773.
Solar ultraviolet (sUV) radiation remains a major cause of skin aging. Nelumbo nucifera (lotus) is a well-known edible plant widely grown in Asia, including Korea, China, and Japan. The lotus consists of flowers, leaves, stems, and seeds, and all parts reportedly possess nutritional and medical values. Traditionally, lotus flowers, leaves, stems, and seeds have been used as antidiarrheal agents, diuretics, antipyretics, and antimicrobial and antihyperlipidemic agents. In addition, the Nelumbo nucifera lotus embryo has been shown to possess sedative and antipyretic properties and can relieve hemostatic thirst and treat eye diseases. Recently, Nelumbo nucifera lotus flower extract has been widely used in cosmetics due to its ability to reduce wrinkles and its whitening effects. Numerous cosmetics using Nelumbo nucifera lotus embryo extracts are commercially available. However, the active components of Nelumbo nucifera remain elusive. Lotusine is a phytochemical and soluble alkaloid found in lotus embryos. Herein, we examined the anti-wrinkle effect of Lotusine using sUV-exposed human keratinocytes. We observed that Lotusine reduced sUV-induced matrix metalloproteinase (MMP)-1 expression and modulated transcriptional activities of activator protein (AP)-1 and nuclear factor kappa B (NF-κB). sUV-induced AP-1 and NF-κB activity could be activated via multiple signal transduction cascades, including the p38 MAPK, JNK, ERK1/2, and Akt pathways in the skin. Lotusine inhibited the MEK1/2-ERK1/2-p90RSK, MKK3/6-p38, and Akt-p70S6K pathways. Overall, our findings suggest that Lotusine has potential benefits related to MMP-1 expression and skin aging following sUV exposure. Hence, the lotus can be developed as a valuable functional food and cosmetic material.
Insight into Lotusine and Puerarin in Repairing Alcohol-Induced Metabolic Disorder Based on UPLC-MS/MS
Int J Mol Sci 2022 Sep 8;23(18):10385.PMID:36142292DOI:10.3390/ijms231810385.
Alcohol is an essential element in human culture. However, alcoholism has contributed to numerous health issues, including alcoholic fatty liver and sudden death. We found that the alkaloid Lotusine possessed hepato- and neuroprotection against alcohol injuries. Lotusine showed comparable protective effects to puerarin, a widely recognized antagonist against alcohol damage. To better understand the metabolic response to alcohol injury and antagonist molecules, we applied sensitive zebrafish and LC-ESI-MS to collect metabolites related to alcohol, puerarin and Lotusine exposure. LC-MS identified 119 metabolites with important physiological roles. Differential metabolomic analysis showed that alcohol caused abnormal expression of 82 metabolites (60 up-regulated and 22 down-regulated). These differential metabolites involved 18 metabolic pathways and modules, including apoptosis, necroptosis, nucleotide and fatty acid metabolism. Puerarin reversed seven metabolite variations induced by alcohol, which were related to necroptosis and sphingolipid metabolism. Lotusine was found to repair five metabolites disorders invoked by alcohol, mainly through nucleotide metabolism and glutathione metabolism. In phenotypic bioassay, Lotusine showed similar activities to puerarin in alleviating behavioral abnormalities, neuroapoptosis and hepatic lipid accumulation induced by alcohol exposure. Our findings provided a new antagonist, Lotusine, for alcohol-induced damage and explored the roles in repairing abnormal metabolism.
Lotusine, an alkaloid from Nelumbo nucifera (Gaertn.), attenuates doxorubicin-induced toxicity in embryonically derived H9c2 cells
In Vitro Cell Dev Biol Anim 2020 May;56(5):367-377.PMID:32468412DOI:10.1007/s11626-020-00466-1.
Cardiotoxicity is the major challenge in chemotherapy with doxorubicin (DOX) or adriamycin. Doxorubicin manifests oxidative stress via an uncontrolled progression of reactive oxygen species in cardiomyocytes; thereby, dysregulation and dysfunction of myocardium thus lead to apoptosis. Several attempts have been made to overcome this side effect in patients with antioxidant-rich supplements to control the free radicals. Plant-based or plant-derived compounds pay more attention to cure such complications in patients for supporting the treatment, revitalizing or regulating the normal metabolism. Hence, our study focused on pretreatment of embryonically derived rat cardiomyocytes (H9c2) with phytocompound Lotusine to prevent DOX-mediated oxidative stress. From the experiment, the DOX-exposed cells have shown morphological abnormalities such as reduced cell size, shrinkage, blebbing, and chromatin condensation, whereas no such deformities were observed in lotusine-pretreated cells even after the exposure to DOX. Increased endogenous antioxidants with reduced lipid peroxidation were observed in lotusine-pretreated cells, whereas the antioxidants were reduced along with increased lipid peroxidation in doxorubicin-exposed cells. A decreased reactive oxygen species generation was evidenced with the 2',7'-dichlorofluorescein diacetate (DCF-DA) staining method. In qPCR analysis, the lotusine-pretreated cells have mitigated doxorubicin-mediated apoptosis by downregulating the pro-apoptotic gene Bax and apoptotic executor caspase-3. It was further confirmed with the luminometric assay, which resulted in lesser luminescence in lotusine-pretreated cells, whereas higher luminescence was recorded in doxorubicin-alone-treated cells. In conclusion, the present study revealed that the Lotusine pretreatment has exhibited potential cardioprotective activity against DOX-induced oxidative stress by increasing the intracellular antioxidant defense.
Lotusine G: a new cyclopeptide alkaloid from Zizyphus lotus
Fitoterapia 2002 Feb;73(1):63-8.PMID:11864766DOI:10.1016/s0367-326x(01)00363-x.
In addition to six cyclopeptide alkaloids lotusines A-F, a new compound Lotusine G, was isolated from the root bark of Zizyphus lotus by centrifugal partition chromatography.
NMR spectroscopic analysis of Lotusine
Magn Reson Chem 2005 Feb;43(2):184-5.PMID:15562520DOI:10.1002/mrc.1510.
Lotusine, a soluble alkaloid, is one of the major constituents of the Chinese medicine Lotus Plumule, and has antihypertension and antibacterial activity. In order to confirm the structures reported in the literature by explicit 1H and 13C assignments, we applied a series of NMR experiments including 1H-1H COSY, HSQC and HMBC.