Lipo3000 Transfection Reagent
目录号 : GK20006Lipo3000转染试剂包含脂质体纳米颗粒技术，可提供优异的转染性能，并提高相关应用的结果和重复性
Sample solution is provided at 25 µL, 10mM.
- View current batch:
Preparation Before Experiment
• Plasmid DNA (0.5–5 μg/μL stock solution)
• Opti-MEM reduced serum medium
• Microcentrifuge tube
Experimental Steps
A. Super Lipo3000 DNA Transfection Reagent requirements
| Component | 96-well | 24-well | 6-well |
| Final DNA per well | 100 ng | 500 ng | 2500 ng |
| Final Lipo3000 Reagent per well | 0.15 and 0.3 μL | 0.75 and 1.5 μL | 3.75 and 7.5 μL |
| Final P-3000 Reagent per well | 0.2 μL | 1.0 μL | 5.0 μL |
B. Transfection of siRNA
When transfecting siRNA into cells, follow the DNA protocol described above, but do not add P-3000 when diluting siRNA (Step 3)
C. SuperLipo3000DNA Transfection Reagent Protocol
Transfect the cells according to the table below, using the specified volume of DNA and P-3000 reagent and the corresponding two volumes of Lipo3000 (when optimized). The volume of each reaction mixture is the volume of a single well, and the pipetting difference is considered. Calculate the volume of other holes in proportion.
Lipo3000 Transfection Reagent contains liposome nanoparticle technology, which can provide excellent transfection performance and improve the results and reproducibility of related applications. This reagent has excellent transfection efficiency and can improve cell activity, and is widely used in difficult to transfect and common cell types (such as HEK293 and Hela cells). In the process of successfully transfecting a wide range of cell types related to biological research, this reagent can：
• Provides excellent performance-our reagents that achieve the highest transfection efficiency for cell types that are difficult to transfect (the cell types that can be efficiently transfected include: fibroblasts (3T3, COS-7), myoblasts (C2C12 , L6 CRL-1458), liver cells (HepG2, HuH7), red leukemia cells (K562), breast cancer (MCF7, Hs578T), prostate cancer (LNCap), lung cancer cells (A549, NCI-H460), osteosarcoma cells ( U-2 OS, Saos-2), colon cancer cells (Caco2, SW480), pancreatic cancer cells (PANC-1) Skin melanoma cells(SK-MEL-28)
• Improve cell activity-gentle on cells and very low toxicity (this liposome dosage can maintain a high transfection efficiency in a wide dynamic range, and can be optimized easily and quickly as needed. For related applications where damage is minimized, the use of low-toxic liposomes is recommended)
• Variety of functions-also suitable for DNA, RNA and co-transfection applications (this liposome is used for new genome editing related applications. Through efficient transfection, it can increase the success of TALEN or CRISPR system shearing and recombination Probability will ultimately maximize the efficiency of genetic modification and simplify subsequent processing steps)
Lipo3000转染试剂应用脂质体纳米颗粒技术，提供优异的转染性能，并提高相关应用结果的重复性。该试剂具有优异的转染效率，可以提高细胞活性，广泛用于难以转染和常见的细胞类型（如HEK293和Hela细胞）。在成功转染与生物学研究相关的多种细胞类型的过程中，该试剂可以：•转染难以转染的细胞类型（可有效转染的细胞包括：成纤维细胞（3T3、COS-7）、成肌细胞（C2C12、L6 CRL-1458）、肝细胞（HepG2、HuH7）、红白血病细胞（K562）、乳腺癌（MCF7、Hs578T）、前列腺癌（LNCap）、，肺癌细胞（A549、NCI-H460）、骨肉瘤细胞（U-2 OS、Saos-2）、结肠癌细胞（Caco2、SW480）、胰腺癌细胞（PANC-1）、皮肤黑色素瘤细胞（SK-MEL-28）•温和地提高细胞活性，试剂毒性极低（该脂质体剂量可在宽动态范围内保持高转染效率，并可根据需要轻松快速地优化。）•也适用于DNA、RNA和共转染应用（该脂质体用于新的基因组编辑相关应用。通过高效转染，它可以提高TALEN或CRISPR系统剪切和重组的成功率。最终，将最大化基因修饰的效率，并简化后续处理步骤）
| Components | 0.25 mL | 0.75 mL |
| Lipo3000 Transfection Reagent | 0.25 mL | 0.75 mL |
| P-3000 Reagent | 0.25 mL | 0.75 mL |
| Store at 4°C. Do not freeze. | ||