Lipo3000 Transfection Reagent
目录号 : GK20006GMax™ Liposome3000 转染试剂含有脂质体纳米粒子技术，可以提供出色的转染性能，并改善相关应用的结果和可重复性。
Sample solution is provided at 25 µL, 10mM.
- View current batch:
Preparation Before Experiment
• Plasmid DNA (0.5–5 μg/μL stock solution)
• Opti-MEM reduced serum medium
• Microcentrifuge tube
Experimental Steps
A. Super Lipo3000 DNA Transfection Reagent requirements
| Component | 96-well | 24-well | 6-well |
| Final DNA per well | 100 ng | 500 ng | 2500 ng |
| Final Lipo3000 Reagent per well | 0.15 and 0.3 μL | 0.75 and 1.5 μL | 3.75 and 7.5 μL |
| Final P-3000 Reagent per well | 0.2 μL | 1.0 μL | 5.0 μL |
B. Transfection of siRNA
When transfecting siRNA into cells, follow the DNA protocol described above, but do not add P-3000 when diluting siRNA (Step 3)
C. SuperLipo3000DNA Transfection Reagent Protocol
Transfect the cells according to the table below, using the specified volume of DNA and P-3000 reagent and the corresponding two volumes of Lipo3000 (when optimized). The volume of each reaction mixture is the volume of a single well, and the pipetting difference is considered. Calculate the volume of other holes in proportion.
GMax™ Liposome3000 Transfection Reagent contains liposome nanoparticle technology, which can provide excellent transfection performance and improve the results and reproducibility of related applications. This reagent has excellent transfection efficiency and can improve cell activity, and is widely used in difficult to transfect and common cell types (such as HEK293 and Hela cells). In the process of successfully transfecting a wide range of cell types related to biological research, this reagent can:
• Provides excellent performance-our reagents that achieve the highest transfection efficiency for cell types that are difficult to transfect (the cell types that can be efficiently transfected include: fibroblasts (3T3, COS-7), myoblasts (C2C12 , L6 CRL-1458), liver cells (HepG2, HuH7), red leukemia cells (K562), breast cancer (MCF7, Hs578T), prostate cancer (LNCap), lung cancer cells (A549, NCI-H460), osteosarcoma cells ( U-2 OS, Saos-2), colon cancer cells (Caco2, SW480), pancreatic cancer cells (PANC-1) Skin melanoma cells(SK-MEL-28)
• Improve cell activity-gentle on cells and very low toxicity (this liposome dosage can maintain a high transfection efficiency in a wide dynamic range, and can be optimized easily and quickly as needed. For related applications where damage is minimized, the use of low-toxic liposomes is recommended)
GMax™ Liposome 3000 转染试剂采用了脂质体纳米技术，能够提供出色的转染性能，并改善相关应用的结果和可重复性。该试剂具有优异的转染效率，可以提高细胞活性，在难以转染和常见细胞类型（如HEK293和Hela细胞）中得到广泛应用。在成功地转染与生物研究相关的各种细胞类型过程中，该试剂可以实现以下功能：
• 提供卓越表现 - 我们的试剂对于难以转染的细胞类型达到最高的转染效率（可以有效转染包括成纤维细胞（3T3、COS-7）、肌母细胞（C2C12、L6 CRL-1458）、肝脏细胞（HepG2、HuH7）、红白血病细胞（K562）、乳腺癌(MCF7, Hs578T)、前列腺癌(LNCap)、肺癌(A549, NCI-H460)、骨肉瘤(U-2 OS, Saos-2)、结直肠癌(Caco2, SW480)、胰腺癌(PANC-1)，皮肤黑素瘤(SK-MEL-28)等细胞类型）。
• 提高细胞活性 - 对细胞温和，毒性极低（该脂质体剂量可以在广泛的动态范围内保持高转染效率，并可根据需要轻松快速地进行优化。对于最小化损伤的相关应用，建议使用低毒性脂质体）。
| Components | 0.25 mL | 0.75 mL |
| Lipo3000 Transfection Reagent | 0.25 mL | 0.75 mL |
| P-3000 Reagent | 0.25 mL | 0.75 mL |
| Store at 4°C. Do not freeze. | ||