Lipo2000 Transfection Reagent
目录号 : GK20005GMax™ Liposome2000 转染试剂是一种专有配方，可用于将核酸转染到各种真核细胞中。
Sample solution is provided at 25 µL, 10mM.
- View current batch:
Preparation Before Experiment
1.Plate cells so they will be 70–90% confluent at the time of transfection.
2.Prepare plasmid DNA-lipid complexes.
3.Add DNA-lipid complexes to cells.
Experimental Steps
A. Lipo2000 DNA Transfection Reagent Protocol
| Component | 96-well | 24-well | 6-well |
| Final DNA per well | 100 ng | 500 ng | 2500 ng |
| Final Lipo2000 Reagent per well | 0.2–0.5 μL | 1.0–2.5 μL | 5.0–12.5 μL |
B. Co-Transfection of Plasmid DNA and siRNA
Transfect plasmid DNA and siRNA at the same time using Lipo2000 Reagent by adding 30 pmol (~0.6 μg) of siRNA per 1 μg of DNA.
C. mRNA Transfection
mRNA can be transfected in a 24-well plate using Lipo2000 Reagent by adding 0.5–1 μg of mRNA per well.
D. Lipo2000 DNA Transfection Reagent Protocol
Transfect cells according to the following chart. Volumes are given on a per-well basis. Each reaction mix is sufficient for triplicate (96-well), duplicate (24-well), and single well (6-well) transfections, and accounts for pipetting variations.
GMax™ Liposome2000 Transfection Reagent is a proprietary formulation for transfecting nucleic acids into a wide range of eukaryotic cells. DNA-GMax™ Liposome2000 complexes must be made in serum-free medium such as Opti-MEM® Reduced Serum Medium and can be added directly to cells in culture medium, in the presence or absence of serum/antibiotic. It is not necessary to remove complexes or change/add medium after transfection. The amount of GMax™ Liposome2000 Reagent required for successful transfection varies depending on the cell type and passage number. Start any new transfection by testing the recommended four concentrations of GMax™ Liposome2000 Reagent to determine an optimum amount.
GMax™ Liposome2000 转染试剂是一种专有配方，可将核酸转染到各种真核细胞中。DNA-GMax™ Liposome2000 复合物必须在无血清培养基（如 Opti-MEM® Reduced Serum Medium）中制备，并可以直接加入含或不含血清/抗生素的培养基中的细胞。转染后无需去除复合物或更换/添加培养基。成功转染所需的 GMax™ Liposome2000 试剂量因细胞类型和通行证号而异。通过测试推荐的四个浓度来确定最佳用量开始任何新的转染实验。
| Components | 0.75 mL | 1.5 mL |
| Lipo2000 Transfection Reagent | 0.75 mL | 1.5 mL |
| Store at 4°C. Do not freeze. | ||