Lipo2000 Transfection Reagent
目录号 : GK20005Lipo2000转染试剂是一种专利配方，用于将核酸转染到广泛的真核细胞中。
Sample solution is provided at 25 µL, 10mM.
- View current batch:
Preparation Before Experiment
1.Plate cells so they will be 70–90% confluent at the time of transfection.
2.Prepare plasmid DNA-lipid complexes.
3.Add DNA-lipid complexes to cells.
Experimental Steps
A. Lipo2000 DNA Transfection Reagent Protocol
| Component | 96-well | 24-well | 6-well |
| Final DNA per well | 100 ng | 500 ng | 2500 ng |
| Final Lipo2000 Reagent per well | 0.2–0.5 μL | 1.0–2.5 μL | 5.0–12.5 μL |
B. Co-Transfection of Plasmid DNA and siRNA
Transfect plasmid DNA and siRNA at the same time using Lipo2000 Reagent by adding 30 pmol (~0.6 μg) of siRNA per 1 μg of DNA.
C. mRNA Transfection
mRNA can be transfected in a 24-well plate using Lipo2000 Reagent by adding 0.5–1 μg of mRNA per well.
D. Lipo2000 DNA Transfection Reagent Protocol
Transfect cells according to the following chart. Volumes are given on a per-well basis. Each reaction mix is sufficient for triplicate (96-well), duplicate (24-well), and single well (6-well) transfections, and accounts for pipetting variations.
Lipo2000 Transfection Reagent is a proprietary formulation for transfecting nucleic acids into a wide range of eukaryotic cells. DNA-Lipo 2000 complexes must be made in serum-free medium such as Opti-MEM® Reduced Serum Medium and can be added directly to cells in culture medium, in the presence or absence of serum/antibiotic. It is not necessary to remove complexes or change/add medium after transfection. The amount of Lipo 2000 Reagent required for successful transfection varies depending on the cell type and passage number. Start any new transfection by testing the recommended four concentrations of Lipo2000 Reagent to determine an optimum amount.
Lipo2000转染试剂（Lipo2000 Transfection Reagent）是一种专利制剂，用于将核酸转染到真核细胞中。DNA Lipo 2000复合物必须在无血清培养基（如Opti-MEM®还原血清培养基）中制备，并且可以在存在或不存在血清/抗生素的情况下直接添加到培养的细胞中。转染后无需去除复合物或更换/添加培养基。成功转染所需的Lipo 2000试剂的量取决于细胞类型和传代数。通过测试推荐的四种浓度的Lipo2000试剂来开始新的转染，以确定最佳量。
| Components | 0.75 mL | 1.5 mL |
| Lipo2000 Transfection Reagent | 0.75 mL | 1.5 mL |
| Store at 4°C. Do not freeze. | ||