Lactoyl Phenylalanine
(Synonyms: Lac-Phe, N-Lactoyl-Phenylalanine) 目录号 : GC52331
Lactoyl Phenylalanine是一种由乳酸和苯丙氨酸通过胞质非特异性二肽酶（CNDP 2）形成的次级代谢产物。
Cas No.:183241-73-8
Sample solution is provided at 25 µL, 10mM.
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Lactoyl Phenylalanine is a secondary metabolite formed by the action of cytoplasmic non-specific dipeptidase (CNDP 2) between lactic acid and phenylalanine [1]. Lactoyl Phenylalanine can reduce obesity and improve glucose tolerance by inhibiting appetite and maintaining energy balance [2].
In vivo, Lactoyl Phenylalanine (50mg/kg/d; i.p.; 30 days) specifically inhibits energy intake in obese mice without affecting energy expenditure pathways, and improves blood sugar balance, while these effects are not observed in lean mice [2]. Lactoyl Phenylalanine (10, 20, 30mg/kg/day; intravenous) treatment for 7 days can significantly improve the motor function of mice and significantly reduce fat accumulation at the injured site [3].
References:
[1] Hinton Jr A, Neikirk K, Le H, et al. N-lactoyl phenylalanine suppresses appetite and obesity with important implications for aging and age-related diseases[J]. Aging advances, 2024, 1(2): 172-173.
[2] Li VL, He Y, Contrepois K, Liu H, Kim JT, Wiggenhorn AL, Tanzo JT, Tung AS, Lyu X, Zushin PH, Jansen RS, Michael B, Loh KY, Yang AC, Carl CS, Voldstedlund CT, Wei W, Terrell SM, Moeller BC, Arthur RM, Wallis GA, van de Wetering K, Stahl A, Kiens B, Richter EA, Banik SM, Snyder MP, Xu Y, Long JZ. An exercise-inducible metabolite that suppresses feeding and obesity. Nature. 2022 Jun;606(7915):785-790.
[3] Ying W, Weng W, Wang P, Pan C, Qiu J, Huang Q, Zhan G, Chen X. N-Lactoyl-Phenylalanine modulates lipid metabolism in microglia/macrophage via the AMPK-PGC1α-PPARγ pathway to promote recovery in mice with spinal cord injury. J Neuroinflammation. 2025 Jun 27;22(1):167.
Lactoyl Phenylalanine是一种由乳酸和苯丙氨酸通过胞质非特异性二肽酶（CNDP 2）形成的次级代谢产物 [1]。Lactoyl Phenylalanine能够通过抑制食欲和维持能量平衡的作用来减少肥胖并改善葡萄糖耐量 [2]。
在体内，Lactoyl Phenylalanine (50mg/kg/d, i.p.) 治疗肥胖小鼠30天，能够特异性地抑制能量摄入而不影响能量消耗途径，并且改善血糖平衡，而这些效果在瘦小鼠中并未表现出来 [2]。Lactoyl Phenylalanine（10, 20, 30mg/kg/day; i.v.）治疗7天能够显著改善小鼠的运动功能，并显著减少受伤部位的脂肪堆积 [3] 。
| Animal experiment [1]: | |
Animal models | C57BL/6 |
Preparation Method | The C57BL/6 mice were divided into 6 groups: Sham group (in which mice had their vertebral laminae pried open but without spinal cord injury, n = 24), SCI group (with spinal cord contusion injury, n = 24), Exercise group (Mice with spinal cord contusion were exercised on a running wheel for 30min each day. Specifically, the protocol involved starting from the second day after surgery, when the mice regained consciousness from anesthesia and the ability to move freely, and then continuously running on the wheel for 30min each day, n = 12), L-P group (After the establishment of the spinal cord contusion model, a dose of 20mg/kg of Lactoyl Phenylalanine was administered via the tail vein every day for a week. The first administration was immediately carried out after the SCI model was established and the wound was sutured. n = 18). 10mg/kg L-P group (After the establishment of the spinal cord contusion model, a dose of 10mg/kg of Lactoyl Phenylalanine was administered via the tail vein every day for a week, n = 6). 30mg/kg L-P group (After the establishment of the spinal cord contusion model, a dose of 30mg/kg of Lactoyl Phenylalanine was administered via the tail vein every day for a week, n = 6). |
Dosage form | 10, 20, 30mg/kg/day for 1 week; i.v. |
Applications | Lactoyl Phenylalanine can significantly improve the movement function of mice and significantly reduce fat accumulation in the injured area. The therapeutic effect of Lactoyl Phenylalanine reaches its peak at a dose of 20 milligrams per kilogram. |
References: | |
| Cas No. | 183241-73-8 | SDF | Download SDF |
| 别名 | Lac-Phe, N-Lactoyl-Phenylalanine | ||
| Canonical SMILES | C[C@H](O)C(N[C@H](C(O)=O)CC1=CC=CC=C1)=O | ||
| 分子式 | C12H15NO4 | 分子量 | 237.3 |
| 溶解度 | DMF: 25 mg/mL,DMSO: 14 mg/mL,Ethanol: 20 mg/mL,PBS (pH 7.2): 0.30 mg/mL | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.2141 mL | 21.0704 mL | 42.1408 mL |
| 5 mM | 0.8428 mL | 4.2141 mL | 8.4282 mL |
| 10 mM | 0.4214 mL | 2.107 mL | 4.2141 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet