L-Ascorbic Acid 2-phosphate (magnesium salt hydrate)
(Synonyms: 2-Phospho-L-ascorbic acid magnesium hydrate) 目录号 : GC47538
L-Ascorbic Acid 2-phosphate (magnesium salt hydrate)是一种常用于细胞和组织疗法原料的维生素C衍生物。
Cas No.:1713265-25-8
Sample solution is provided at 25 µL, 10mM.
L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) is a vitamin C derivative usually employed as a raw material in cell and tissue therapy[1]. L-Ascorbic Acid 2-phosphate is commonly used as a substrate for alkaline phosphatase assay[2].
In vitro, L-Ascorbic Acid 2-phosphate treatment (1000μM) for 3 weeks significantly stimulated the proliferation of human skin fibroblasts and induced collagen synthesis[3]. L-Ascorbic Acid 2-phosphate treatment (300μM) for 2 weeks significantly stimulated the growth of human corneal endothelial cells, maintained the characteristic polygonal morphology, reduced the generation of 8-hydroxy-2-deoxyguanosine (8-OHdG), and prevented oxidative DNA damage[4]. After 30 days of culture in the presence of L-Ascorbic Acid 2-phosphate (100μM), the growth of rabbit corneal cells was significantly increased, and the cells had multilayer characteristics[5]. L-Ascorbic Acid 2-phosphate (250μM) treatment for 7 days significantly inhibited the growth of adipose-derived stem cells seeded at low density (1,250 cells/cm2), resulting in decreased expression of antioxidant enzymes[6]. L-Ascorbic Acid 2-phosphate treatment (50μM; 24h) significantly and sustainably improved intracellular L-ascorbic acid and enhanced type 1 collagen synthesis and mRNA expression in human gingival fibroblasts, with reduction of intracellular reactive oxygen species (ROS)[7].
References:
[1] Xu X, Woźniczka M, Van Hecke K, et al. Structural study of L-ascorbic acid 2-phosphate magnesium, a raw material in cell and tissue therapy[J]. JBIC Journal of Biological Inorganic Chemistry, 2020, 25(6): 875-885.
[2] Wu Y, Chen W, Wang C, et al. Assays for alkaline phosphatase that use L-ascorbic acid 2-phosphate as a substrate[J]. Coordination Chemistry Reviews, 2023, 495: 215370.
[3] Hata R I, Senoo H. L‐ascorbic acid 2‐phosphate stimulates collagen accumulation, cell proliferation, and formation of a three‐dimensional tissuelike substance by skin fibroblasts[J]. Journal of cellular physiology, 1989, 138(1): 8-16.
[4] Shima N, Kimoto M, Yamaguchi M, et al. Increased proliferation and replicative lifespan of isolated human corneal endothelial cells with L-ascorbic acid 2-phosphate[J]. Investigative ophthalmology & visual science, 2011, 52(12): 8711-8717.
[5] Saika S, Kanagawa R, Uenoyama K, et al. L-ascorbic acid 2-phosphate, a phosphate derivative of L-ascorbic acid, enhances the growth of cultured rabbit keratocytes[J]. Graefe's archive for clinical and experimental ophthalmology, 1991, 229(1): 79-83.
[6] Wu Y K, Tu Y K, Yu J, et al. The influence of cell culture density on the cytotoxicity of adipose-derived stem cells induced by L-ascorbic acid-2-phosphate[J]. Scientific Reports, 2020, 10(1): 104.
[7] Tsutsumi K, Fujikawa H, Kajikawa T, et al. Effects of L‐ascorbic acid 2‐phosphate magnesium salt on the properties of human gingival fibroblasts[J]. Journal of periodontal research, 2012, 47(2): 263-271.
L-Ascorbic Acid 2-phosphate (magnesium salt hydrate)是一种常用于细胞和组织疗法原料的维生素C衍生物[1]。L-Ascorbic Acid 2-phosphate常作为碱性磷酸酶检测的底物[2]。
在体外，1000μM浓度的L-Ascorbic Acid 2-phosphate处理3周可显著促进人皮肤成纤维细胞增殖并诱导胶原合成[3]。300μM浓度的L-Ascorbic Acid 2-phosphate处理2周能有效刺激人角膜内皮细胞生长，维持特征性多边形形态，减少8-羟基-2-脱氧鸟苷(8-OHdG)生成，预防氧化DNA损伤[4]。100μM浓度的L-Ascorbic Acid 2-phosphate培养30天使兔角膜细胞生长显著增加，并呈现多层特征[5]。250μM浓度的L-Ascorbic Acid 2-phosphate处理7天会显著抑制低密度接种（1,250细胞/cm2）的脂肪干细胞生长，导致抗氧化酶表达降低[6]。50μM浓度的L-Ascorbic Acid 2-phosphate处理24小时可持续提升人牙龈成纤维细胞内L-抗坏血酸水平，增强I型胶原合成及mRNA表达，同时降低细胞内活性氧(ROS)[7]。
| Cell experiment [1]: | |
Cell lines | Human corneal endothelial cells |
Preparation Method | Human corneal endothelial cells (HCECs) were seeded at a density of 500 cells/cm2 in 12-well plates coated with bovine atelocollagen (50μg/mL) in basal medium (Low-glucose Dulbecco's modified Eagle's medium supplemented with 15% fetal calf serum and antibiotics), with or without the addition of the following factors: L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) (0.1, 0.3, 0.6, 0.9, 1.2 and 1.5mM), human basic fibroblast growth factor (bFGF) (2ng/mL), human epidermal growth factor (EGF) (5ng/mL), and insulin (20μg/mL). The cells were maintained in a 5% CO2 incubator at 37°C for 2 weeks, with the medium being changed every other day. After digestion with 0.05% trypsin/EDTA, the cells were counted. All experiments were carried out in triplicate with cells from at least three different donors. |
Reaction Conditions | 0.1, 0.3, 0.6, 0.9, 1.2, and 1.5mM; 2 weeks |
Applications | L-Ascorbic Acid 2-phosphate significantly promoted cell growth of HCECs in a dose-dependent manner. |
References: | |
| Cas No. | 1713265-25-8 | SDF | |
| 别名 | 2-Phospho-L-ascorbic acid magnesium hydrate | ||
| Canonical SMILES | O=C1C(OP(O)(O)=O)=C(O)[C@]([C@@H](O)CO)([H])O1.O=C2C(OP(O)(O)=O)=C(O)[C@]([C@@H](O)CO)([H])O2.[Mg].[Mg].[Mg].O | ||
| 分子式 | C6H6O9P.1.5Mg [XH2O] | 分子量 | 289.6 |
| 溶解度 | PBS (pH 7.2): 1 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.453 mL | 17.2652 mL | 34.5304 mL |
| 5 mM | 690.6 μL | 3.453 mL | 6.9061 mL |
| 10 mM | 345.3 μL | 1.7265 mL | 3.453 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
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