Kaempferol-3-rutinoside
(Synonyms: 莰菲醇-3-O-芸香糖苷) 目录号 : GN10672
A flavonoid
Cas No.:17650-84-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Cell experiment: |
Cortical neurons are treated with nicotiflorin (25-100 mg/mL) just before hypoxia. Cell viability is determined using the MTT assay[2]. |
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Animal experiment: |
Rats[2]A neurological examination is performed on each rat 24 h after the onset of ischemia. Briefly, neurological scores (NS) are derived using a 10-point sliding scale. Rats are administered 2.5, 5, and 10 mg/kg nicotiflorin or vehicle immediately after occlusion. Each animal is examined for reduced resistance to lateral push (score, 4), open field circling (score, 3), and shoulder adduction (score, 2) or contralateral forelimb flexion (score, 1) when held by the tail and suspended approximately 0.5 m above the floor. Rats extending both forelimbs toward the floor and not showing any other signs of neurological impairment are scored 0[2]. |
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References: [1]. Lal Shyaula S, et al. Synthesis and antiglycation activity of kaempferol-3-O-rutinoside (nicotiflorin). Med Chem. 2012 May;8(3):415-20. |
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IC50: 2.9 μM for antilipase effect
Kaempferol-3-O-rutinoside has been isolated from a crude ethyl acetate extract of A. mahidoliae leaves, which could enhance the in vitro migration of keratinocytes. Kaempferol and its glycosides have been reported to possess wound healing activity. Kaempferol and kaempferol-3-O-glucoside have been used in excision and incision wound healing in Wistar rats. Others from Ophioglossum vulgatum also showed wound healing activity via accelerating the cell migration of keratinocytes.
In vitro: It was found that Kaempferol-3-O-rutinoside was a potent in vitro inhibitor of α-glucosidase with over 8-times more activity than acarbose, which was the reference antidiabetic drug. In addition, Kaempferol-3-O-rutinoside displayed a synergistic effect with kaempferol and quercetin, two less potent flavonoid aglycones. However, the SAR profile of such drugs or drug combinations should be further discussed [1].
In vivo: Kaempferol-3-O-rutinoside and rutin were previously tested on normotensive pentothal anaesthetized rats for their cardiovascular activities. Results showed that kaempferol-3-O-rutinoside displayed a remarkable decrease in diastolic, systolic, mean arterial blood pressure as well as heart rate, while rutin showed no effect on any of these parameters [2].
Clinical trial: N/A
References:
[1] Habtemariam S. A-glucosidase inhibitory activity of kaempferol-3-O-rutinoside. Nat Prod Commun.2011 Feb;6(2):201-3.
[2] Mansoor Ahmad,Anwar-Ul-Hassan Gilani,Khalid Aftaband Viqar Uddin Ahmad. Effects of kaempferol-3-O-rutinoside on rat blood pressure. Phytotherapy Research. Volume 7,Issue 4,pages 314–316.
| Cas No. | 17650-84-9 | SDF | |
| 别名 | 莰菲醇-3-O-芸香糖苷 | ||
| 化学名 | 5,7-dihydroxy-2-(4-hydroxyphenyl)-3-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxymethyl]oxan-2-yl]oxychromen-4-one | ||
| Canonical SMILES | CC1C(C(C(C(O1)OCC2C(C(C(C(O2)OC3=C(OC4=CC(=CC(=C4C3=O)O)O)C5=CC=C(C=C5)O)O)O)O)O)O)O | ||
| 分子式 | C27H30O15 | 分子量 | 594.52 |
| 溶解度 | ≥ 59.5mg/mL in DMSO | 储存条件 | Store at 2-8°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.682 mL | 8.4101 mL | 16.8203 mL |
| 5 mM | 0.3364 mL | 1.682 mL | 3.3641 mL |
| 10 mM | 0.1682 mL | 0.841 mL | 1.682 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。