iMAC2
目录号 : GC12046iMAC2 是一种有效的 MAC 抑制剂，IC50 为 28 nM，LD50 为 15000 nM。
Cas No.:335166-00-2
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
iMAC2 is the inhibitor of mitochondrial apoptosis-induced channel (MAC) with the IC50 value of 28 nM[1]. MAC is a putative cytochrome c release channel involved in the intrinsic apoptotic pathway. MAC has been associated with oligomeric, not monomeric, Bax[2]. By blocking MAC and suppressing cytochrome c release, iMAC2 suppressed the onset of apoptotic markers [1].
In vitro: In cultured apoptotic FL5.12 cells, pre-treatment with iMAC2 resulted in a normal volume and residual Annexin V/PI staining. iMAC2 reduced apoptosis by more than 50%. Pre-treatment with iMAC2 induced a significant protection at low micromolar levels (IC50 = 2.5 μM). Pre-incubation with iMAC2 and iMAC3 essentially eliminated the release of cytochrome c (P < 0.0001) while nanomolar levels of iMAC2 completely blocked the current flow through MAC. iMAC2 inhibited release of cytochrome c by Bid-induced Bax activation with the IC50 values of 0.68 μM [1].
References:
[1] Peixoto PM1, Ryu SY, Bombrun A, Antonsson B, Kinnally KW. MAC inhibitors suppress mitochondrial apoptosis.Biochem J. 2009 Oct 12;423(3):381-7.
[2] Dejean L M, Martinez-Caballero S, Guo L, et al. Oligomeric Bax is a component of the putative cytochrome c release channel MAC, mitochondrial apoptosis-induced channel[J]. Molecular biology of the cell, 2005, 16(5): 2424-2432.
| Cas No. | 335166-00-2 | SDF | |
| 化学名 | 3,6-dibromo-9-(2-fluoro-3-(piperazin-1-yl)propyl)-9H-carbazole | ||
| Canonical SMILES | FC(CN1CCNCC1)CN2C3=C(C=C(Br)C=C3)C4=C2C=CC(Br)=C4 | ||
| 分子式 | C19H20Br2FN3.2HCl | 分子量 | 542.11 |
| 溶解度 | DMSO: 2.71 mg/mL | 储存条件 | Desiccate at RT |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8446 mL | 9.2232 mL | 18.4464 mL |
| 5 mM | 0.3689 mL | 1.8446 mL | 3.6893 mL |
| 10 mM | 0.1845 mL | 0.9223 mL | 1.8446 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。