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IL 13 Variant Human

目录号 : GP20559

Interleukin-13 Variant Human Recombinant

IL 13 Variant Human Chemical Structure

规格 价格 库存 购买数量
10μg
¥1,980.00
现货
1mg
¥47,520.00
现货

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Sample solution is provided at 25 µL, 10mM.

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产品描述

Interleukin-13 Variant Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 114 amino acids, with a substitution of Q for R at position 112 compared with the wild type IL-13, having a molecular mass of 12.5 kDa. The IL-13 Variant is purified by proprietary chromatographic techniques.

Product Data

Purity Greater than 95% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. Source Escherichia Coli.
Phycical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Shipping Condition Shipped at Room temp.
Synonyms Interleukin-13; NC30; ALRH; BHR1; P600; IL-13; MGC116786; MGC116788; MGC116789.
Amino Acid Sequence SPGPVPPSTA LRELIEELVN ITQNQKAPLC NGSMVWSINL TAGMYCAALE SLINVSGCSA IEKTQRMLSG FCPHKVSAGQ FSSLHVRDTK IEVAQFVKDL LLHLKKLFRE GQFN.
Solubility It is recommended to reconstitute the lyophilized Interleukin 13 Variant in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Interleukin-13 Variant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IL13 Variant should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Biological Activity The ED50 was determined by the dose dependent prolifiration of TF-1 cells and was found to be 1,000,000 units/mg. This analog has also been shown to exhibit increased in vivo activity compared to wild type IL-13.
Formulation Lyophilized from a 0.2µm filtered concentrated solution in 1xPBS, pH 7.2, containing 5% trehalose.

Introduction

IL13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. IL-13 is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.

Biological Activity

The ED50 was determined by the dose dependent prolifiration of TF-1 cells and was found to be 1,000,000 units/mg. This analog has also been shown to exhibit increased in vivo activity compared to wild type IL-13.

Stability

Lyophilized Interleukin-13 Variant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IL13 Variant should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.