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Hydroxystilbamidine bis(methanesulfonate) Sale

(Synonyms: 羟基脒双(甲磺酸)) 目录号 : GC32195

A fluorescent neuronal retrograde tracer and nucleic acid dye

Hydroxystilbamidine bis(methanesulfonate) Chemical Structure

Cas No.:223769-64-0

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1 mg
¥765.00
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实验参考方法

Kinase experiment:

Silkwork larvae are used in this study. Larvae on the fourth or fifth day of the fifth instar (~4.2 g body weight) are injected with 35 μL of a solution of 10 mg/mL cyclobeximidein H2O. After 5 min, the animals are immobilized in ice, and posterior silk glands are dissected and washed in ice-cold 0.15 M NaCl, 0.015 M Na citrate, 100 μg/mL cycloheximide. Washed glands from two larvae are placed in a homogenizer containing 4.7 mL of 40 mM triethanolamine-HCl, pH 7.5, 0.15 M sucrose, 0.1 M KCI, 3 mM MgCl2, 2 mM reduced glutathione, 10 μg/mL cycloheximide, 750 μg/mL Escherichia coli tRNA, and an appropriate concentration of RNase inhibitor (sodium heparin, 1.5 μg/mL orHydroxystilbamidine bis(methanesulfonate), 1.5 mM)[1].

Animal experiment:

Mice (six per group) are given various doses of Hydroxystilbamidine bis(methanesulfonate) (HSB) 3, 2, and 1 day before antigen. Other groups are given Hydroxystilbamidine bis(methanesulfonate) 1 or 2 days after the injection of antigen. Another group of mice receive antigen and Hydroxystilbamidine bis(methanesulfonate) simultaneously. A control group receives only antigen. The antigen dose consists of 2×108 sheep erythrocytes (SRBC). Four days after the injection of SRBC, the mice are sacrificed and spleens are removed and assayed for plaque-forming cell (PFC) by the plaque assay[2].

References:

[1]. Lizardi PM. Isolation of giant silk fibroin polysomes and fibroin mRNP particles using a novel ribonuclease inhibitor, hydroxystilbamidine. J Cell Biol. 1980 Oct;87(1):292-6.
[2]. Folds JD, et al. Immunosuppression by hydroxystilbamidine isethionate, a lysosome-stabilizing, anti-proteolytic, antifungal drug. Infect Immun. 1975 Mar;11(3):441-4.

产品描述

Hydroxystilbamidine is a fluorescent neuronal retrograde tracer that labels the neuronal cell body as well as proximal dendrites.1,2 Hydroxystilbamidine, when visualized in tissue sections, displays excitation/emission maxima of 323/620 nm, respectively. It is also a nucleic acid dye that can be used to label DNA and RNA in fixed or unfixed dead cells.3 It displays an excitation maximum of 360 nm and emission maxima of 450 and 600 nm when bound to DNA but only emits at 450 nm when bound to RNA.

1.Akhavan, M., Hoang, T.X., and Havton, L.A.Improved detection of fluorogold-labeled neurons in long-term studiesJ. Neurosci. Methods152(1-2)156-162(2006) 2.Li, C., Marshall, C.T., Lu, C., et al.The dynamic distribution of fluoro-gold and its interrelation with neural nitric oxide synthase following intracerebroventricular injection into rat brainBiotech. Histochem.81(1)41-50(2006) 3.Festy, B., and Daune, M.Hydroxystilbamidine. A nonintercalating drug as a probe of nucleic acid conformationBiochemistry12(24)4827-4834(1973)

Chemical Properties

Cas No. 223769-64-0 SDF
别名 羟基脒双(甲磺酸)
Canonical SMILES NC(C1=CC=C(/C=C/C2=C(O)C=C(C(N)=N)C=C2)C=C1)=N.OS(=O)(C)=O.OS(=O)(C)=O
分子式 C18H24N4O7S2 分子量 472.54
溶解度 Water: soluble 储存条件 -20°C, protect from light
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1 mM 2.1162 mL 10.5811 mL 21.1622 mL
5 mM 0.4232 mL 2.1162 mL 4.2324 mL
10 mM 0.2116 mL 1.0581 mL 2.1162 mL
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Research Update

EFFECTS OF TRICAINE methanesulfonate IN A MANAGED COLLECTION OF MOON JELLYFISH ( AURELIA AURITA)

J Zoo Wildl Med 2022 Mar;53(1):100-107.PMID:35339154DOI:10.1638/2021-0028.

The moon jellyfish (Aurelia aurita) is a scyphozoan frequently maintained in public and private aquaria. Little research has been conducted to investigate the effects of various drugs, such as anesthetics, in this species. Tricaine methanesulfonate (MS-222), a common immersion anesthetic for fish and amphibians, was evaluated in a managed population of moon jellyfish. Twenty-four clinically healthy jellyfish were assigned into three groups of eight for trials of 0.3 g/L MS-222 (low concentration [LC]), 0.6 g/L MS-222 (high concentration [HC]), and a saltwater control. The goal was to evaluate the effects of MS-222 administration on moon jellyfish movement and response to stimuli. Movement and response to stimuli were measured via rocking and probe stimulus tests and observations of bell contraction quality and body tone. These tests were performed at baseline and throughout both drug exposure and recovery periods. A threshold drug effect was defined based on systematic scoring criteria. Additionally, elastomer tags were administered to four of eight animals in each MS-222 group to evaluate response to tag placement after drug exposure. Threshold drug effect was achieved in six of eight individuals in the LC group and eight of eight individuals in the HC group. The LC group had median threshold and recovery times of 12.2 and 10.1 min, respectively, while the HC group had median threshold and recovery times of 4.0 and 19.9 min, respectively. The HC group had significantly faster time to threshold drug effect (P < 0.001) and longer recovery times (P= 0.005) than the LC group. In both the LC and HC tagged group, three of four jellyfish had no reaction to tag placement. All animals recovered uneventfully, and there were no mortalities. MS-222 at 0.3 and 0.6 g/L decreased movement and response to stimuli in moon jellyfish.

Determination of Methyl methanesulfonate and Ethyl Methylsulfonate in New Drug for the Treatment of Fatty Liver Using Derivatization Followed by High-Performance Liquid Chromatography with Ultraviolet Detection

Molecules 2022 Mar 17;27(6):1950.PMID:35335314DOI:10.3390/molecules27061950.

A new derivatization high-performance liquid chromatography method with ultraviolet detection was developed and validated for the quantitative analysis of methanesulfonate genotoxic impurities in an innovative drug for the treatment of non-alcoholic fatty liver disease. In this study, sodium dibenzyldithiocarbamate was used as a derivatization reagent for the first time to enhance the sensitivity of the analysis, and NaOH aqueous solution was chosen as a pH regulator to avoid the interference of the drug matrix. Several key experimental parameters of the derivatization reaction were investigated and optimized. In addition, specificity, linearity, precision, stability, and accuracy were validated. The determined results of the samples were consistent with those obtained from the derivatization gas chromatography-mass spectrometry analysis. Thus, the proposed method is a reliable and practical protocol for the determination of trace methanesulfonate genotoxic impurities in drugs containing mesylate groups.

methanesulfonate (MSA) Catabolic Genes from Marine and Estuarine Bacteria

PLoS One 2015 May 15;10(5):e0125735.PMID:25978049DOI:10.1371/journal.pone.0125735.

Quantitatively, methanesulfonate (MSA) is a very relevant compound in the global biogeochemical sulfur cycle. Its utilization by bacteria as a source of carbon and energy has been described and a specific enzyme, methanesulfonate monooxygenase (MSAMO), has been found to perform the first catabolic step of its oxidation. Other proteins seemingly involved in the import of MSA into bacterial cells have been reported. In this study, we obtained novel sequences of genes msmA and msmE from marine, estuary and soil MSA-degraders (encoding the large subunit of the MSAMO enzyme and the periplasmic component of the import system, respectively). We also obtained whole-genome sequences of two novel marine Filomicrobium strains, Y and W, and annotated two full msm operons in these genomes. Furthermore, msmA and msmE sequences were amplified from North Atlantic seawater and analyzed. Good conservation of the MsmA deduced protein sequence was observed in both cultured strains and metagenomic clones. A long spacer sequence in the Rieske-type [2Fe-2S] cluster-binding motif within MsmA was found to be conserved in all instances, supporting the hypothesis that this feature is specific to the large (α) subunit of the MSAMO enzyme. The msmE gene was more difficult to amplify, from both cultivated isolates and marine metagenomic DNA. However, 3 novel msmE sequences were obtained from isolated strains and one directly from seawater. With both genes, our results combined with previous metagenomic analyses seem to imply that moderate to high-GC strains are somehow favored during enrichment and isolation of MSA-utilizing bacteria, while the majority of msm genes obtained by cultivation-independent methods have low levels of GC%, which is a clear example of the misrepresentation of natural populations that culturing, more often than not, entails. Nevertheless, the data obtained in this work show that MSA-degrading bacteria are abundant in surface seawater, which suggests ecological relevance for this metabolic group of bacteria.

Determination of methyl methanesulfonate and ethyl methanesulfonate in methanesulfonic acid by derivatization followed by high-performance liquid chromatography with ultraviolet detection

J Sep Sci 2017 Sep;40(17):3414-3421.PMID:28675589DOI:10.1002/jssc.201700543.

Methanesulfonic acid is routinely used in pharmaceuticals but can contain potentially genotoxic impurities such as methyl methanesulfonate and ethyl methanesulfonate. The aim of this study was to develop a simple high-performance liquid chromatography with ultraviolet detection method for determining methyl methanesulfonate and ethyl methanesulfonate in methanesulfonic acid. Samples (250 mg) in water/acetonitrile (200 μL) were first combined with 10.0 mol/L sodium hydroxide solution (270 μL). Then they were mixed with 2.0 mg/mL N,N-diethyldithiocarbamate (500 μL), diluted to 5 mL with N,N-dimethylacetamide and allowed to react at 80°C for 1 h. The derivatives were analyzed using gradient high-performance liquid chromatography with ultraviolet detection (277 nm) and structurally elucidated by liquid chromatography with mass spectrometry. With acetonitrile/5 mmol/L ammonium acetate solution as the eluent and 1 mL/min as the flow rate on a C18 column, the derivatives were eluted at 10.6 and 14.8 min. Good linearity (correlation coefficients > 0.999) and low limits of quantitation (0.6 ppm) were obtained. The recoveries were in the range of 80-115% with relative standard deviation < 5.0%. Finally, the established method was successfully used for the determination of methyl methanesulfonate and ethyl methanesulfonate in methanesulfonic acid.

Escherichia coli utilizes methanesulfonate and L-cysteate as sole sulfur sources for growth

FEMS Microbiol Lett 2001 Dec 18;205(2):271-5.PMID:11750815DOI:10.1111/j.1574-6968.2001.tb10960.x.

Twenty-three Escherichia coli strains were tested for their ability to use taurine, methanesulfonate, L-cysteate and other alkanesulfonates as sole sulfur sources for growth. One strain was unable to use any of the alkanesulfonates offered as sole sulfur sources for growth but grew with sulfate. Seven strains (class I) used alkanesulfonates for this purpose, but not methanesulfonate or L-cysteate. A further seven strains (class II) grew with all compounds tested, except with L-cysteate, and eight strains (class III) utilized all compounds tested as sulfur sources. Sulfur assimilation from methanesulfonate and L-cysteate was absolutely dependent on the ssuEADCB operon that encodes an alkanesulfonate uptake system (SsuABC) and a two-component monooxygenase (SsuDE) involved in the release of sulfite from alkanesulfonates. Long-term exposure of class I strains to methanesulfonate and of class II strains to L-cysteate selected for derivatives that utilized these two sulfur sources as efficiently as sulfate. The nucleotide sequence of the ssuEADCB operon in the methanesulfonate- and L-cysteate-utilizing derivative EC1250Me+ was identical to that in the class I wild-type EC1250. Gain of the ability to utilize methanesulfonate and L-cysteate as sulfur sources thus appears to result from increased expression of ssu genes rather than from a change in the quality of one or several of the Ssu proteins.