HT-2 Toxin
(Synonyms: HT-2毒素) 目录号 : GC18185HT-2 Toxin是 T-2 Toxin的三萜真菌代谢产物，可抑制真核细胞的蛋白质合成，导致细胞凋亡、免疫抑制和组织损伤。
Cas No.:26934-87-2
Sample solution is provided at 25 µL, 10mM.
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HT-2 Toxin, a triterpenoid fungal metabolite of T-2 toxin, inhibits protein synthesis in eukaryotic cells, resulting in apoptosis, immunosuppression, and tissue damage [1-3].
In porcine brain capillary endothelial cells, the cell viability of cells incubated with HT-2 Toxin (1nM-10µM; 24h, 48h) decreased in a concentration-dependent manner [4]. In porcine oocytes, exposure to HT-2 Toxin (10nM, 50nM, 100nM; 44h) causes oxidative stress induced apoptosis/autophagy [5]. In human chondrocytes, exposure to HT-2 Toxin (20ng/mL; 48h) significantly reduced chondrocyte viability [6]. In porcine oocytes, HT-2 Toxin (100nM; 48h) impairs porcine oocyte in vitro maturation through disruption of endomembrane system [7].
In C57BL/6J mice, the body weight of mice exposed to HT-2 Toxin (1.6mg/kg, 3.2mg/kg; ig; 4 weeks) decreased in a dose-dependent manner [8]. In rat, HT-2 Toxin (0.01mg/kg, 0.1mg/kg, 0.5mg/kg, 1mg/kg; ip; 96h) elicited anorectic effects of varying durations in a dose-dependent manner [9].
References:
[1]. Nathanail AV, Varga E, Meng-Reiterer J, et al. Metabolism of the Fusarium mycotoxins T-2 toxin and HT-2 toxin in wheat. Journal of agricultural and food chemistry. 2015 Sep 9; 63(35): 7862-7872.
[2]. Meneely J, Greer B, Kolawole O, et al. T-2 and HT-2 toxins: toxicity, occurrence and analysis: a review. Toxins. 2023 Jul 29; 15(8): 481.
[3]. Zhu CC, Zhang Y, Duan X, et al. Toxic effects of HT-2 toxin on mouse oocytes and its possible mechanisms. Archives of toxicology. 2016 Jun; 90: 1495-1505.
[4]. Weidner M, Hüwel S, Ebert F, et al. Influence of T-2 and HT-2 toxin on the blood-brain barrier in vitro: new experimental hints for neurotoxic effects. PloS one. 2013 Mar 27; 8(3): e60484.
[5]. Zhang Y, Han J, Zhu CC, et al. Exposure to HT-2 toxin causes oxidative stress induced apoptosis/autophagy in porcine oocytes. Scientific reports. 2016 Sep 23; 6(1): 33904.
[6]. Yu FF, Lin XL, Wang X, et al Comparison of apoptosis and autophagy in human chondrocytes induced by the T-2 and HT-2 toxins. Toxins. 2019 May 8; 11(5): 260.
[7]. Li JR, Wu SL, Hu LL, et al. HT-2 toxin impairs porcine oocyte in vitro maturation through disruption of endomembrane system. Theriogenology. 2024 Sep 15; 226: 286-293.
[8]. Hao S, Yao C, Meng P, et al. The spinal consequences of HT-2 toxin and selenium deficiency during bone maturation in mice. Mycotoxin research. 2025 Feb; 41(1): 77-91.
[9]. Zhang J, Zhang H, Liu S, et al. Comparison of anorectic potencies of type A trichothecenes T-2 toxin, HT-2 toxin, diacetoxyscirpenol, and neosolaniol. Toxins. 2018 Apr 29; 10(5): 179.
HT-2 Toxin是 T-2 Toxin的三萜真菌代谢产物，可抑制真核细胞的蛋白质合成，导致细胞凋亡、免疫抑制和组织损伤 [1-3]。
在猪脑毛细血管内皮细胞中，与HT-2 Toxin（1nM-10µM；24h、48h）孵育的细胞活力呈浓度依赖性降低 [4]。在猪卵母细胞中，暴露于HT-2 Toxin（10nM、50nM、100nM；44h）可导致氧化应激诱导的细胞凋亡/自噬 [5]。在人软骨细胞中，暴露于HT-2 Toxin（20ng/mL；48h）可显著降低软骨细胞活力 [6]。在猪卵母细胞中，HT-2 Toxin（100nM；48h）通过破坏内膜系统阻碍猪卵母细胞体外成熟 [7]。
在C57BL/6J小鼠中，暴露于HT-2 Toxin（1.6mg/kg、3.2mg/kg；ig；4周）的小鼠体重呈剂量依赖性下降 [8]。在大鼠中，HT-2 Toxin（0.01mg/kg、0.1mg/kg、0.5mg/kg、1mg/kg；ip；96h）以剂量依赖性方式引起不同持续时间的厌食效应 [9]。
| Cell experiment [1]: | |
Cell lines | Porcine brain capillary endothelial cells |
Preparation Method | Porcine brain capillary endothelial cells were seeded on Collagen-G-coated 96-well plates (250,000cells/cm2) and cultured according to the treatment on Transwell® filter systems (DIV 2: plating medium replaced by serum-free medium on DIV 4 for 2 days). Incubation with various T-2 and HT-2 Toxin concentrations (1nM-10µM) was carried out over 24h and 48h following the addition of the WST-8 solution [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] and incubation according to the manufacturer's manual. |
Reaction Conditions | 1nM-10µM; 24h, 48h |
Applications | The cell viability of cells incubated with HT-2 Toxin decreased in a concentration-dependent manner. |
| Animal experiment [2]: | |
Animal models | C57BL/6J mice |
Preparation Method | Male C57BL/6J mice were subjected to subchronic toxin exposure. A total of 48 mice were divided into six groups, based on toxin concentrations determined in preliminary studies. The groups included a control group, a low selenium diet group, two groups exposed to different concentrations of HT-2 Toxin (1.6mg/kg and 3.2mg/kg), and two groups combining a low selenium diet with each of these HT-2 Toxin dosages. Over a 4-week period, all mice received their designated treatments via oral gavage, mimicking typical clinical and environmental exposure scenarios. |
Dosage form | 1.6mg/kg, 3.2mg/kg; ig; 4 weeks |
Applications | The body weight of mice exposed to HT-2 Toxin decreased in a dose-dependent manner. |
References: | |
| Cas No. | 26934-87-2 | SDF | |
| 别名 | HT-2毒素 | ||
| 化学名 | 12,13-epoxy-trichothec-9-ene-3α,4β,8α,15-tetrol, 15-acetate 8-(3-methylbutanoate) | ||
| Canonical SMILES | CC1=C[C@]2([H])[C@]([C@]([C@H](O)[C@H]3O)(C)[C@@]4(OC4)[C@]3([H])O2)(COC(C)=O)C[C@@H]1OC(CC(C)C)=O | ||
| 分子式 | C22H32O8 | 分子量 | 424.5 |
| 溶解度 | Dichloromethane: 5 mg/ml,DMSO: soluble,Ethanol: soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.3557 mL | 11.7786 mL | 23.5571 mL |
| 5 mM | 0.4711 mL | 2.3557 mL | 4.7114 mL |
| 10 mM | 0.2356 mL | 1.1779 mL | 2.3557 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
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