N-Acetyl-DL-methionine
(Synonyms: N-乙酰-DL-甲硫氨酸) 目录号 : GC38295
N-Acetyl-DL-methionine is an active endogenous metabolite that decreases the hepatic glutathione level in male Bom:NMRI mice.
Cas No.:1115-47-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
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- Purity: >98.00%
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- SDS (Safety Data Sheet)
- Datasheet
N-Acetyl-DL-methionine is an active endogenous metabolite that decreases the hepatic glutathione level in male Bom:NMRI mice.
[1] L A Skoglund, et al. Gen Pharmacol. 1986;17(6):647-9.
| Cas No. | 1115-47-5 | SDF | |
| 别名 | N-乙酰-DL-甲硫氨酸 | ||
| Canonical SMILES | OC(C(CCSC)NC(C)=O)=O | ||
| 分子式 | C7H13NO3S | 分子量 | 191.25 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.2288 mL | 26.1438 mL | 52.2876 mL |
| 5 mM | 1.0458 mL | 5.2288 mL | 10.4575 mL |
| 10 mM | 0.5229 mL | 2.6144 mL | 5.2288 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Effects of N-Acetyl-DL-methionine on the liver, GSH synthesis and plasma ALAT level in male Bom:NMRI mice
Gen Pharmacol 1986;17(6):647-9.PMID:3817431DOI:10.1016/0306-3623(86)90294-6.
A single high peroral dose of N-Acetyl-DL-methionine (859.5 mg/kg) was administered to Bom:NMRI male mice using methylcellulose as a drug vehicle. The administration of a single high peroral dose of N-Acetyl-DL-methionine (859.5 mg/kg) to Bom:NMRI male mice caused an inhibition of the hepatic glutathione decrease compared to a control group treated with methylcellulose only. N-Acetyl-DL-methionine caused a reduction and time delay of the hepatic glutathione decrease found in the group treated with methylcellulose only. There was no observed cellular damage of the liver or kidneys assessed histologically or by plasma ALAT after dosing with a racemic mixture of N-Acetyl-DL-methionine.
In vivo studies on toxic effects of concurrent administration of paracetamol and its N-Acetyl-DL-methionine ester (SUR 2647 combination)
Gen Pharmacol 1988;19(2):213-7.PMID:3350331DOI:10.1016/0306-3623(88)90063-8.
1. Single p.o. doses of paracetamol 400 and 800 mg/kg or SUR 2647 combination (free paracetamol + paracetamol-N-acetyl-DL-methionate, paracetamol/methionine ratio 2:1) equivalent to paracetamol 400 and 800 mg/kg were given to Bom:NMRI mice. Vehicle treated (1% w/v aqueous methylcellulose) mice were established as a control group. 2. All treatment groups irrespective of medication caused an initial GSH depletion. However, SUR 2647 combination 400 mg/kg caused a much earlier hepatic GSH recovery than paracetamol 400 mg/kg. SUR 2647 combination 800 mg/kg caused a higher hepatic GSH level than paracetamol 800 mg/kg. 3. There was no significant difference in the plasma ALAT level after SUR 2647 combination 400 or 800 mg/kg and the control group. Paracetamol 400 and 800 mg/kg caused significant plasma ALAT elevations compared to the control group. 4. The addition of N-Acetyl-DL-methionine esterified to paracetamol, as in the SUR 2647 combination, enhances the hepatic GSH synthesizing capacity in Bom:NMRI mice after experimental overdosage and offers protection of hepatic cell integrity as assessed by plasma ALAT level compared to paracetamol alone.
Plasma concentration of paracetamol and its major metabolites after p.o. dosing with paracetamol or concurrent administration of paracetamol and its N-Acetyl-DL-methionine ester in mice
Gen Pharmacol 1992 Mar;23(2):155-8.PMID:1639228DOI:10.1016/0306-3623(92)90002-2.
1. Single doses of paracetamol 400 (PAR 400) and 800 mg/kg (PAR 800), SUR 2647 combination (free paracetamol + paracetamol-N-acetyl-DL-methionate, paracetamol/methionine ratio 2:1) equivalent to PAR 400 (SURc 400) and PAR 800 (SURc 800) were given p.o. to male Bom:NMRI mice. 2. The objective was to compare the plasma concentrations of free paracetamol and the major metabolites paracetamol-sulphate and paracetamol-glucuronide for a 6 hr period after each test drug. 3. There was no significant difference between PAR 400 and SURc 400 with respect to plasma paracetamol, paracetamol-glucuronide and paracetamol-sulphate concentration with the exception of lower plasma paracetamol concentration (P less than 0.03) at 3 hr following PAR 400. 4. There was no significant difference between PAR 800 and SURc 800 with respect to plasma paracetamol, paracetamol-glucuronide and paracetamol-sulphate concentrations with the exception of lower plasma paracetamol-glucuronide concentration (P less than 0.03) at 4 hr after dosing following SURc 800. 5. Combining free paracetamol and its methionine ester does not seem to alter the pattern of plasma paracetamol, paracetamol-glucuronide and paracetamol-sulphate compared to equal doses of free paracetamol alone after p.o. administration of toxic doses to male Bom:NMRI mice.
Impurity profiling of L-methionine by HPLC on a mixed mode column
J Pharm Biomed Anal 2016 Apr 15;122:118-25.PMID:26852161DOI:10.1016/j.jpba.2016.01.057.
Methionine is mostly produced synthetically. Thus, impurities are synthesis by-products in addition to oxidation and dimerization products. Here, a sensitive HPLC method for the determination of impurities in L-methionine was developed and validated using a SIELC(®) Primesep 100 column. Impurities were separated on the mixed mode column by reversed phase and cationic exchange mechanism. The limit of detection was in the range of 0.06-0.30 μg/ml (0.0004-0.002%), limit of quantification in the range of 0.30-0.75 μg/ml (0.002-0.005%) and linearity was shown in the range of 0.3-30.0 μg/ml (0.002-0.200%). The method was found to be precise (intermediate precision RS <5%; n=2) and accurate (recovery 96.0-121.4%, n=3). The method is also suitable for the purity assessment of DL-methionine and D-methionine. The amount of impurities found in batches was very low. Only L-methionine-sulfoxide and N-Acetyl-DL-methionine could be detected in levels less than 0.05%.
Methionine replacement value of N-acetylmethionine and homocysteinethiolactone hydrochloride for growing rats
J Nutr 1975 May;105(5):577-80.PMID:1127468DOI:10.1093/jn/105.5.577.
The ability of young growing rats to absorb DL-homocysteinethiolactone hydrochloride (HCTL-HCl) and N-Acetyl-DL-methionine (NAM) and to use them for growth when fed a methionine-deficient diet was studied. In situ intestinal absorption studies demonstrated that both compounds are readily absorbed. Intestinal half-times were 24 minutes for NDTL-HCl and 44 minutes for NAM. Adding molar equivalents of 0.2 or 0.4% methionine to a 0.2% methionine basal diet fed ad libitum resulted in 28-day gains that were 83.2 and 91.1%, respectively, for NCTL-HCl and 94.6 and 99.8% for NAM of the average gain resulting when DL-methionine was added at equivalent levels. Feed efficiencies were also significantly (P smaller than 0.05) improved over rats fed the basal diet. Increasing the HCTL-HCl addition to 0.6% reduced feed intake and gain but not feed efficiency.