Hypocrellin C
(Synonyms: 竹红菌丙素) 目录号 : GC36284
Hypocrellin C 是从真菌Hypocrella bambusae 和Shiraia bambusicola 中分离得到的一种色素。
Cas No.:149457-83-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Hypocrellin C is a pigment isolated from the fungi Hypocrella bambusae and Shiraia bambusicola[1].
[1]. Liu XY, et al. High-efficiency biosynthesis of hypocrellin A in Shiraia sp. using gamma-ray mutagenesis. Appl Microbiol Biotechnol. 2016 Jun;100(11):4875-83.
| Cas No. | 149457-83-0 | SDF | |
| 别名 | 竹红菌丙素 | ||
| Canonical SMILES | COC1=C(O)C2=C3C4=C1C(C(C)=O)=C(C)CC5=C4C(C(C(OC)=C6)=C3C(OC)=CC2=O)=C(C(O)=C5OC)C6=O | ||
| 分子式 | C30H24O9 | 分子量 | 528.51 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8921 mL | 9.4606 mL | 18.9211 mL |
| 5 mM | 0.3784 mL | 1.8921 mL | 3.7842 mL |
| 10 mM | 0.1892 mL | 0.9461 mL | 1.8921 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
[Simultaneous determination of hypocrellin A, hypocrellin B, and Hypocrellin C by HPLC]
Zhongguo Zhong Yao Za Zhi 2012 Jan;37(1):75-8.PMID:22741466doi
Objective: To develop a high-performance liquid chromatography (HPLC) method for simultaneous determination of hypocrellin A, hypocrellin B, and Hypocrellin C. Method: The separation was carried out on a Kromasil C18 (4.6 mm x 250 mm, 5 micrm) colum eluted with in mobile phases of water containing 0.5% glacial acetic acid and acetonitrile. The column temperature was 35 degrees C, and the flow rate was 1.0 mL x min(-1). The detection wavelength was set at 265 nm. Result: The three compounds were well separated. Calibration curves of hypocrellin A, hypocrellin B, and Hypocrellin C showed good linear relationship RSD > 2.0%. The average recoveries of the hypocrellin A, hypocrellin B, and Hypocrellin C were 101.8%, 102.3%, 100.0%, respectively. Conclusion: The developed method is simple, accurate, and repeatable, and can be readily used as valid tool for the quality control of Hypocrella bambusae.
Purification of hypocrellins from Shiraia bambusicola by coordinated high-speed countercurrent chromatography using cupric chloride as a complexing agent
J Sep Sci 2021 Apr;44(7):1383-1390.PMID:33471412DOI:10.1002/jssc.202001133.
Hypocrellins are anthraquinone that can act as excellent photosensitizers for photodynamic therapy. In the present work, we found that high-speed countercurrent chromatography using cupric chloride as a complexing agent effectively separated hypocrellins from Shiraia bambusicola extract. The optimal two-phase solvent system consisted of petroleum ether/ethyl acetate/methanol/water (7:3:5.5:4.5, v/v/v/v), with 0.01 mol/L cupric chloride in the lower phase at pH of 2.45. This lower phase served as the mobile phase, whereas the upper phase acted as the stationary phase. Employing a continuous separation mode, three continuous injections were found to allow the purification of 1.2 g of crude extract in approximately 12 h. Hypocrellin B (10.8 mg), hypocrellin A (16.2 mg), and Hypocrellin C (15.6 mg) were obtained from this process. Simulation of complexation of hypocrellin A with divalent copper ion by computational chemistry calculations indicated that three pairs of hydroxyl and carbonyl groups in hypocrellin A had similar binding energies, and demonstrated that hypocrellin A and B owned different metal-to-ligand ratios as compared to Hypocrellin C. These factors could modify the partitioning of these compounds in two-phase solvent system, and resulting in a suitable separation factor. This method would also be used to purify other anthraquinones from natural products.
[Chemical constituents from ethyl acetate extract of Shiaria bambusicola]
Zhongguo Zhong Yao Za Zhi 2013 Apr;38(7):1008-13.PMID:23847947doi
Fourteen compounds were isolated and purified from the ethyl acetate of the ethanol extract of Shiaria bambusicola by various chromatographic methods, and their structures were elucidated by spectral techniques and physicochemical properties as hypocrellin A (1), hypocrellin B (2), Hypocrellin C (3), hypomycin A (4), ergosterol (5), ergosterol peroxide (6), (22E, 24R)-5alpha, 8alpha-epidioxy-6,9(11),22-trien-3beta-ol (7), ergosta-7, 24(28)-dien-3beta-ol (8), (22E, 24R)-ergost-7, 22-dien-3beta, 5alpha, 6beta-triol (9), (22E,24R)-ergosta-7, 22-diene-3beta, 5alpha, 6beta-triol-3-O-palmitate (10), (22E, 24R)-ergosta-7, 22-diene-3beta, 5alpha, 6beta-triol-6-O-palmitate (11), 1-O-monostearin (12), 1, 3-O-distearin (13), and mannitol (14). Among them, compounds 7-13 were firstly isolated from this genus.
[Studies on the chemical constituents of Shiraia bambusicola]
Zhongguo Zhong Yao Za Zhi 2002 Sep;27(9):674-6.PMID:12776569doi
Objective: To study the chemical constituents of Shiraia bambusicola. Method: Column chromatography with silica gel was employed for the isolation and purification of constituents. The structures were elucidated by means of chemical and spectroscopic data. Result: Seven compounds were obtained and identified as hypocrellin A (I), hypocrellin B (II), Hypocrellin C (III), hypomycin A (IV), ergosterol (V), ergosterol peroxide (VI) and 1,8-dihydroxy anthraquinone (VII). Conclusion: Compounds (IV) (VII) were separated from Shiraia bambusicola for the first time.