Surfactin C1
(Synonyms: 表面活性素) 目录号 : GC39448
Surfactin C1是一种高效生物活性表面活性剂, 可显著抑制脂多糖（LPS）诱导的HL-60细胞与人脐静脉内皮细胞（HUVEC）的粘附作用。
Cas No.:24730-31-2
Sample solution is provided at 25 µL, 10mM.
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Surfactin C1 is a highly effective and biologically active surfactant that inhibits HL-60 cell-human umbilical vein endothelial cells (HUVECs) adhesion triggered by lipopolysaccharide (LPS)[1]. Surfactin C1 can suppress LPS-induced expression of ICAM-1 and VCAM-1 in HUVECs[1]. Surfactin C1 protects wheat from the wheat blight pathogen and activates salicylic acid and jasmonic acid-dependent defense responses [2].
References:
[1] Takahashi T, Ohno O, Ikeda Y, et al. Inhibition of lipopolysaccharide activity by a bacterial cyclic lipopeptide surfactin[J]. The Journal of Antibiotics, 2006, 59(1): 35-43.
[2] Le Mire G, Siah A, Brisset M N, et al. Surfactin protects wheat against Zymoseptoria tritici and activates both salicylic acid-and jasmonic acid-dependent defense responses[J]. Agriculture, 2018, 8(1): 11.
Surfactin C1是一种高效生物活性表面活性剂, 可显著抑制脂多糖（LPS）诱导的HL-60细胞与人脐静脉内皮细胞（HUVEC）的粘附作用[1]。Surfactin C1能抑制LPS诱导的HUVECs中ICAM-1和VCAM-1的表达[1]。Surfactin C1可保护小麦免受小麦枯病病原体的侵害，并激活水杨酸和茉莉酸依赖性防御反应[2]。
| Cell experiment [1]: | |
Cell lines | Human umbilical vein endothelial cells (HUVECs) and HL-60 cells |
Preparation Method | HUVECs was cultured in a coated culture dish with type I collagen as the culture medium, and was cultivated in MCDB-131 medium supplemented with 10% heat-inactivated bovine fetal serum (FBS) and 10ng/ml basic fibroblast growth factor (bFGF). 4×104 cells per well of HUVECs were seeded in a 48-well collagen-coated plate and cultured overnight at 37°C and 5% CO2. The cells were pre-incubated with several concentrations of Surfactin C1 (0, 0.3, 1, 3, 10μg/ml) for 2 hours, and then treated with the stimulants (1μg/ml lipopolysaccharide, 1μg/ml lipid A, 10ng/ml tumor necrosis factor-α or 10ng/ml interleukin-1β) for 4 hours. After removing the culture medium, the wells were washed twice with PBS. Next, leukemia cells (HL-60) were added to the treated HUVEC monolayer at a concentration of 6×104 cells per well. After 1 hour, the non-adherent leukemia cells were removed by washing 3 times with PBS. Then, the number of adherent cells was counted in 1 microscopic field. |
Reaction Conditions | 0, 0.3, 1, 3, 10μg/ml; 2h |
Applications | Surfactin C1 decreased HL-60 cell-HUVEC adhesion caused by lipopolysaccharide, without showing any cytotoxicity. |
References: | |
| Cas No. | 24730-31-2 | SDF | |
| 别名 | 表面活性素 | ||
| Canonical SMILES | OC(C[C@H](N1)C(N[C@](C(N[C@H](C(OC(CC(N[C@@H](CCC(O)=O)C(N[C@](CC(C)C)([H])C(N[C@H](CC(C)C)C(N[C@@H](C(C)C)C1=O)=O)=O)=O)=O)CCCCCCCCCC(C)C)=O)CC(C)C)=O)([H])CC(C)C)=O)=O | ||
| 分子式 | C53H93N7O13 | 分子量 | 1036.34 |
| 溶解度 | DMSO: 50 mg/mL (48.25 mM); Water: 31.25 mg/mL (30.15 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 0.9649 mL | 4.8247 mL | 9.6493 mL |
| 5 mM | 0.193 mL | 0.9649 mL | 1.9299 mL |
| 10 mM | 0.0965 mL | 0.4825 mL | 0.9649 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet