DL-Homocystine
(Synonyms: DL-高胱氨酸) 目录号 : GC35877
An oxidized dimeric form of homocysteine
Cas No.:870-93-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
DL-Homocystine is an oxidized dimeric form of homocysteine . It inhibits CDNB-induced L-cystine transport into isolated human erythrocytes by 75% when used at a concentration of 2.5 mM.1 DL-Homocystine increases the number of circulating endothelial cells and permeability of the pulmonary microcirculation and decreases the number of circulating platelets, as well as activates venostatic thrombosis induced by bowel loop ligation in a rat model of homocystinemia, an inborn error of metabolism characterized by a deficiency in cystathionine β-synthase (CBS) that is associated with thrombosis and atherosclerosis.2
1.Ohtsuka, Y., Kondo, T., and Kawakami, Y.Oxidative stresses induced the cystine transport activity in human erythrocytesBiochem. Biophys. Res. Commun.155(1)160-166(1988) 2.Hladovec, J.Experimental homocystinemia, endothelial lesions and thrombosisBlood Vessels16(4)202-205(1979)
| Cas No. | 870-93-9 | SDF | |
| 别名 | DL-高胱氨酸 | ||
| Canonical SMILES | N[C@H](C(O)=O)CCSSCC[C@H](N)C(O)=O.N[C@@H](C(O)=O)CCSSCC[C@@H](N)C(O)=O | ||
| 分子式 | C8H16N2O4S2 | 分子量 | 268.35 |
| 溶解度 | Water: 10 mg/mL (37.26 mM; ultrasonic and adjust pH to 11 with NaOH); DMSO: < 1 mg/mL (insoluble or slightly soluble); Water: < 0.1 mg/mL (insoluble) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.7265 mL | 18.6324 mL | 37.2648 mL |
| 5 mM | 0.7453 mL | 3.7265 mL | 7.453 mL |
| 10 mM | 0.3726 mL | 1.8632 mL | 3.7265 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Sophorajaponica L. flower mediated carbon dots with nitrogen and sulfur co-doped as a sensitive fluorescent probe for amoxicillin detection
Spectrochim Acta A Mol Biomol Spectrosc 2022 Dec 5;282:121703.PMID:35933781DOI:10.1016/j.saa.2022.121703.
This article first reported the green synthesis of N, S co-doped fluorescent carbon dots (N, S-CDs-Sop) and sought to establish the fluorescence detection system for amoxicillin (AMX). By using Sophorajaponica L. flower as the green precursor and DL-Homocystine as the co-dopant, N, S-CDs-Sop were successfully prepared via a one-pot hydrothermal method, exhibiting good water solubility and excellent photoluminescence. It was revealed that the surface of N, S-CDs-Sop was abundant in amino, hydroxyl and carboxyl groups after being characterized by a variety of techniques. When Fe3+ was added, Fe3+ could be complexed with N, S-CDs-Sop to from N, S-CDs-Sop-Fe3+ chelation leading to a significant static quenching of fluorescence. However, when N, S-CDs-Sop, Fe3+ and AMX coexisted, AMX would coordinate with Fe3+ and form the strong chelate due to the favorable chemical structure, resulting in the rapid fluorescence recovery. Such a fast, simple and sensitive fluorescence "off-on" strategy with a low LOD and a relatively wide range was successfully applied to the detection of AMX, which is closely correlated with human health.
Excess levels of cysteine and homocysteine induce tibial dyschondroplasia in broiler chicks
J Nutr 1992 Mar;122(3):482-7.PMID:1542006DOI:10.1093/jn/122.3.482.
The effect of excessive levels of cysteine and homocysteine on tibial dyschondroplasia (TD) in broiler chicks was studied. In the first experiment, graded levels of L-cysteine as well as one level of L-homocysteine were supplemented to a corn-soybean-based diet adequate in sulfur amino acids. Levels equal to or above 0.5% supplemental cysteine increased the incidence of TD, and levels equal to or above 0.75% supplemental cysteine increased the severity of TD above that found in chicks fed the basal diet. Also, L-homocysteine at 0.5% induced TD. In the second experiment, graded levels of DL-Homocystine were added to the basal diet to determine the threshold value of homocystine needed to induce TD, and a level of ammonium sulfate isosulfurous to 0.45% homocystine was added to a basal diet. The results showed that 0.45% DL-Homocystine was the lowest level that increased the severity of TD above that found in chicks fed the basal diet and that sulfate did not induce TD. In the third experiment, a 2 x 2 factorial design was used to investigate the interaction between DL-Homocystine and copper. Copper supplementation lessened the severity of TD caused by DL-Homocystine. Copper supplementation also tended to improve growth, especially in birds fed DL-Homocystine.
Effect of methionine replacement by homocystine in cultures containing both malignant rat breast carcinosarcoma (Walker-256) cells and normal adult rat liver fibroblasts
In Vitro 1975 Jan-Feb;11(1):14-9.PMID:1126735DOI:10.1007/BF02615317.
When malignant W-256 rat breast carcinosarcoma cells are mixed with an equal number of normal adult rat liver fibroblasts and allowed to grow in a medium containing sufficient L-methionine and an excess of vitamin B12 and of folic acid, the malignant cells outgrow the normal cells, and within 2 weeks the tissue culture flasks contain only neoplastic cells. However, when ample DL-Homocystine or homocysteine replaces methionine in the medium containing the same amount of vitamin B12 and folic acid, and seeded with the same type and number of malignant and normal cells, the malignant cells die and the normal cells thrive. Substantiating this conclusion are the results of injections into rats of comparable numbers of cells from each group after 3 weeks of growth in tissue culture. Fatal malignancies are produced by the homocystein-cultivated cells.
Effect of methotrexate with 5-methyltetrahydrofolate rescue and dietary homocystine on survival of leukemic mice and on concentrations of liver adenosylamino acids
Cancer Res 1983 Nov;43(11):5210-6.PMID:6616457doi
We have increased significantly the survival time of DBA/2 mice bearing methionine-dependent L1210 or L5178Y leukemia cells by i.p. administration of lethal doses of methotrexate (five daily doses of 25 mg/kg body weight) followed by rescue with 5-methyl tetrahydrofolate (five daily doses of 20 mg/kg body weight). The mice were maintained on a semipurified choline- and cyst(e)ine-free diet containing 0.32% L-methionine. We further increased significantly the survival time of the treated animals bearing L5178Y cells, but not those bearing L1210 cells, by substitution of 0.86% DL-Homocystine for the methionine in the diet. We have examined the effects of both diets in mice treated with methotrexate and 5-methyl tetrahydrofolate, singly and in combination, on the concentrations of S-adenosylmethionine and S-adenosylhomocysteine in the liver, a tissue highly active in the metabolism of these amino acids. The substitution of homocystine for methionine in the diet of untreated animals led to a significant increase in S-adenosylhomocysteine and decrease in S-adenosylmethionine in the liver, with a resultant profound decrease in the ratio of S-adenosylmethionine to S-adenosylhomocysteine which was not further altered significantly by administration of methotrexate.
Palladium(II) Ion Mediated Disulfide/Thiolate Interconversion: Predicting the Disulfide Group State from First Principles
J Phys Chem A 2019 Jun 13;123(23):4873-4882.PMID:31117586DOI:10.1021/acs.jpca.9b00740.
Different reactivity of homologous disulfides toward Pd2+ was previously reported: stepwise complexation to Pd2+ for l-cystine and cystamine ligands, while for DL-Homocystine and 3,3'-dithiodipropionic acid, disulfide's disproportionation toward thiolate and sulfinic acid complexes is observed. The disulfide/thiolate interconversion of four different disulfide ligands in the presence of nonredox metal cation Pd2+ in aqueous solution has been computationally investigated. We see this different reactivity in different capacities of considered homologous disulfides to stabilize forming S,S'-binuclear complexes, which are believed to be key intermediates toward interconversion products. We thus devise a theoretical model that rationalizes experimentally observed phenomenon of disulfides different reactivity toward nonredox transition metal cation Pd2+.